SIRT1在骨性关节炎滑膜中表达的相关研究
发布时间：2019-04-01 19:04
【摘要】：目的探讨沉默信息调节因子2相关酶1(SIRT1)在骨性关节炎(OA)滑膜组织及细胞中的相关性表达。方法①OA组:因膝骨性关节炎行全膝关节置换术中所取标本共20例,男10例,女10例,年龄62-78岁,中位年龄72.3岁。纳入标准:符合美国风湿病学会2001年推荐OA诊断标准;②正常对照组:因急性外伤致下肢截肢术或因胫骨平台骨折行开放复位内固定术中所取标本10例,男6例,女4例,年龄19-42岁,中位年龄34.2岁。纳入保准:术后病理学诊断关节无病变。标本离体后无菌状态下放置于装有生理盐水的无菌标本盒中,2 h内送实验室进行滑膜细胞的分离与培养。用甲苯胺蓝染色对滑膜细胞进行组织形态学观察,用免疫组化法检测滑膜细胞SIRT1表达及分布特点,用Western blot法检测滑膜组织及细胞中SIRT1表达。结果1、甲苯胺蓝染色显示:滑膜细胞大部分呈梭形,两极胞突细长,末端多与临近细胞相连,交织成网状,网间可见少部分小星型或多边形细胞。正常组与OA组滑膜细胞无明显形态学差异。2、免疫组化结果显示:在正常组及OA组滑膜细胞中SIRT1均可见阳性表达,胞质广泛呈棕黄色,细胞核及细胞膜未见明显黄染,OA组与正常组对比染色强度下降显著,两者之间的差异具有统计学意义(t=20.208,P㩳0.01)。3、Western blot法结果显示:OA患者滑膜组织及细胞中SIRT1表达低于正常组,正常组与OA组灰度值经统计学分析比较,差异有统计学意义(t=7.664,P0.01),滑膜细胞正常组及OA组灰度值经统计学分析比较也差异有统计学意义(t=8.619,P㩳0.01)。结论SIRT1与OA导致滑膜炎的发生发展密切相关,为SIRT1可以作为OA治疗的靶点提供一个新的理论依据。
[Abstract]:Aim to investigate the expression of silencing information regulator 2 related enzyme 1 (SIRT1) in synovial tissue and cells of osteoarthritis (OA). Methods 1OA group: 20 cases of total knee arthroplasty due to osteoarthritis of the knee, including 10 males and 10 females, aged 62-78 years (median age 72.3 years), were taken from the patients with osteoarthritis of the knee (n = 20, male 10, female 10, n = 10). Inclusion criteria: meet the OA diagnostic criteria recommended by the American Society of Rheumatology in 2001; 2 normal control group: 10 specimens, 6 males and 4 females, aged 19-42 years (median age 34.2 years), were taken from amputation of lower extremities due to acute trauma or open reduction and internal fixation due to tibial plateau fracture. Inclusion accuracy: postoperative pathological diagnosis of joints without pathological changes. The specimens were placed in an aseptic specimen box containing normal saline in vitro and sent to the laboratory for isolation and culture of synovial cells within 2 hours. The histological morphology of synovial cells was observed by toluidine blue staining. The expression and distribution of SIRT1 in synovial cells were detected by immunohistochemical method, and the expression of SIRT1 in synovial tissues and cells were detected by Western blot method. The results were as follows: 1. Most of the synovial cells were fusiform with long and slender polar processes. The terminal of synovial cells were mostly connected with adjacent cells and interwoven into reticulation. A few small star-shaped or polygonal cells could be seen in the intermeshwork between the synovial cells. There was no significant difference between normal group and OA group in morphology of synovial cells. 2. Immunohistochemical results showed that SIRT1 was expressed in synovial cells in both normal group and OA group, cytoplasm was widely brown, and no obvious yellow staining was found in nucleus and cell membrane, and there was no obvious yellow staining in nucleus and cell membrane of synovial cells in normal group and OA group. The staining intensity of OA group was significantly lower than that of normal group (t = 20.208, P < 0.01). The results of western blot showed that the expression of SIRT1 in synovial tissue and cells of OA patients was lower than that of normal group, and the expression of SIRT1 in synovial tissue and cells of OA patients was significantly lower than that of normal group (P < 0.01). The gray values of normal group and OA group were statistically significant (t = 7.664, P0.01). The gray values of normal group and OA group were also statistically significant (t = 8.619, P < 0.01), and that of normal synovial cell group and OA group was significantly higher than that of normal group (t = 7.664, P0.01). (P < 0.01). Conclusion SIRT1 is closely related to the occurrence and development of synovitis caused by OA, which provides a new theoretical basis for SIRT1 as a target of OA therapy.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R684.3
本文编号：2451800
[Abstract]:Aim to investigate the expression of silencing information regulator 2 related enzyme 1 (SIRT1) in synovial tissue and cells of osteoarthritis (OA). Methods 1OA group: 20 cases of total knee arthroplasty due to osteoarthritis of the knee, including 10 males and 10 females, aged 62-78 years (median age 72.3 years), were taken from the patients with osteoarthritis of the knee (n = 20, male 10, female 10, n = 10). Inclusion criteria: meet the OA diagnostic criteria recommended by the American Society of Rheumatology in 2001; 2 normal control group: 10 specimens, 6 males and 4 females, aged 19-42 years (median age 34.2 years), were taken from amputation of lower extremities due to acute trauma or open reduction and internal fixation due to tibial plateau fracture. Inclusion accuracy: postoperative pathological diagnosis of joints without pathological changes. The specimens were placed in an aseptic specimen box containing normal saline in vitro and sent to the laboratory for isolation and culture of synovial cells within 2 hours. The histological morphology of synovial cells was observed by toluidine blue staining. The expression and distribution of SIRT1 in synovial cells were detected by immunohistochemical method, and the expression of SIRT1 in synovial tissues and cells were detected by Western blot method. The results were as follows: 1. Most of the synovial cells were fusiform with long and slender polar processes. The terminal of synovial cells were mostly connected with adjacent cells and interwoven into reticulation. A few small star-shaped or polygonal cells could be seen in the intermeshwork between the synovial cells. There was no significant difference between normal group and OA group in morphology of synovial cells. 2. Immunohistochemical results showed that SIRT1 was expressed in synovial cells in both normal group and OA group, cytoplasm was widely brown, and no obvious yellow staining was found in nucleus and cell membrane, and there was no obvious yellow staining in nucleus and cell membrane of synovial cells in normal group and OA group. The staining intensity of OA group was significantly lower than that of normal group (t = 20.208, P < 0.01). The results of western blot showed that the expression of SIRT1 in synovial tissue and cells of OA patients was lower than that of normal group, and the expression of SIRT1 in synovial tissue and cells of OA patients was significantly lower than that of normal group (P < 0.01). The gray values of normal group and OA group were statistically significant (t = 7.664, P0.01). The gray values of normal group and OA group were also statistically significant (t = 8.619, P < 0.01), and that of normal synovial cell group and OA group was significantly higher than that of normal group (t = 7.664, P0.01). (P < 0.01). Conclusion SIRT1 is closely related to the occurrence and development of synovitis caused by OA, which provides a new theoretical basis for SIRT1 as a target of OA therapy.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R684.3
【参考文献】
相关期刊论文 前1条
1 吕大鹏;白伦浩;孔冉冉;李毓卓;王勇;;MMP-7、MMP-9及其抑制剂TIMP-3在骨性关节炎患者膝关节滑膜中的表达和意义[J];中国医科大学学报;2009年04期
，本文编号：2451800
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