复合PRP与BMP-4的Nano-HA并髓心减压治疗兔股骨头坏死

发布时间：2019-05-06 12:51

【摘要】：目的探讨富含血小板血浆(platelet rich plasma,PRP)与骨形态发生蛋白-4(Bone morphogenetic protein-4,BMP-4)的纳米羟基磷灰石(Nano hydroxyapatite,Nano-HA)联合髓心减压修复兔股骨头坏死的可行性。方法激素法制备新西兰大白兔股骨头坏死模型,取36只随机分为A、B、C共3组,各12只。A组为模型组,不进行任何处理;B组行单纯髓心减压术;C组髓心减压后植入富含PRP与BMP-4的Nano-HA。分别于术后2、4、8周处死大白兔,取股骨头进行组织学观察及骨小梁图像分析,并在第8周测定股骨头骨密度。并用spss软件对数据进行分析。结果(1)组织学观察显示,从术后第2周开始C组的成骨能力一直好于A、B组,C组术后2周时开始出现骨小梁,术后8周时骨小梁已成熟,骨髓组织形成;B组术后8周时缺损区仍然在修复,可见骨髓组织;A组术后8周时仍无骨组织。(2)骨小梁图像分析显示,C组术后2、4、8周骨小梁所占缺损区面积比例均高于B组,差异有显著性(P0.05)。(3)骨密度测定显示,术后8周C组的骨密度高于B组及A组,差异有显著性(P0.05)。结论PRP协同BMP-4可诱导坏死的股骨头内新生骨的形成,Nano-HA可作为髓心减压后修复股骨头坏死良好的支架材料,PRP协同BMP-4搭载Nano-HA联合髓心减压能加速兔股骨头坏死修复。
[Abstract]:Aim to investigate the feasibility of platelet-rich plasma (platelet rich plasma,PRP and bone morphogenetic protein-4 (Bone morphogenetic protein-4,BMP-4 nano-hydroxyapatite (Nano hydroxyapatite,Nano-HA) combined with medullary decompression in the repair of femoral head necrosis in rabbits. Methods the femoral head necrosis model of New Zealand white rabbits was made by hormone method. 36 rabbits were randomly divided into 3 groups, group A (n = 12), group B (n = 12) without any treatment, group B (n = 12) and group B (n = 12), and group B (n = 12, n = 12). Group C implanted Nano-HA. rich in PRP and BMP-4 after decompression. The rabbits were killed at 2, 4 and 8 weeks after operation. The femoral head was taken for histological observation and trabecular image analysis, and the bone mineral density of the femoral head was measured at the 8th week. Spss software was used to analyze the data. Results (1) histological observation showed that the osteogenic ability of group C was better than that of group A from the second week after operation, group B, group C appeared trabecular bone at 2 weeks after operation, bone trabecular matured at 8 weeks after operation, and bone marrow tissue formed. In group B, the defect area was still repaired at 8 weeks after operation, and bone marrow tissue could be seen. There was no bone tissue in group A at 8 weeks after operation. (2) the ratio of trabecular defect area in group C was higher than that in group B at 2,4,8 weeks after operation, and the difference was significant (P0.05). (3). 8 weeks after operation, bone mineral density in group C was significantly higher than that in group B and A (P0.05). Conclusion PRP combined with BMP-4 can induce the formation of new bone in necrotic femoral head. Nano-HA can be used as a good scaffold for repairing osteonecrosis of femoral head after decompression of medullary heart. PRP and BMP-4 combined with Nano-HA combined with decompression of medulla can accelerate the repair of femoral head necrosis in rabbits.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R681.8

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