兔前交叉韧带不同分束断裂对内侧半月板的影响

发布时间：2019-05-27 12:33

【摘要】：目的探讨兔前交叉韧带(ACL)不同分束断裂对内侧半月板的影响。方法 36只新西兰大白兔随机分为ACL前内侧束(AMB)断裂组、后外侧束(PLB)断裂组及未断裂组,每组12只。分别制备兔右后肢AMB断裂、PLB断裂及未断裂模型,取第4、6、8周为观测点,记录兔术侧膝关节功能评分,取术侧半月板行组织学切片苏木精-伊红染色,免疫组织化学法检测半月板中金属基质蛋白酶13(MMP-13)表达情况。结果术后4、6、8周未断裂组兔术侧半月板胶原纤维、软骨细胞形态正常,无血管增生,无炎性细胞,胶原纤维束粗大,排列整齐;AMB断裂组及PLB断裂组兔术侧半月板表面逐渐出现不平整、组织疏松,胶原纤维排列紊乱,炎性细胞浸润。术后4、6、8周未断裂组兔术侧膝关节功能评分差异无统计学意义(P0.05);ABM断裂组和PLB断裂组兔术后6、8周的术侧膝关节功能评分均低于4周时(P0.05),8周的术侧膝关节功能评分均低于6周时(P0.05);同时间点比较,AMB断裂组和PLB断裂组兔术侧膝关节功能评分均较未断裂组显著降低(P0.05);AMB断裂组与PLB断裂组同时间点比较术侧膝关节功能评分差异无统计学意义(P0.05)。未断裂组兔术后第4、6、8周半月板MMP-13阳性细胞百分率差异无统计学意义(P0.05)。AMB断裂组、PLB断裂组兔术后6、8周的MMP-13阳性细胞百分率均高于4周时(P0.05),8周的MMP-13阳性细胞百分率均高于6周时(P0.05)。相同时间点AMB断裂组、PLB断裂组兔MMP-13阳性细胞百分率均高于未断裂组(P0.05),相同时间点AMB断裂组兔MMP-13阳性细胞百分率高于PLB断裂组(P0.05)。结论兔前交叉韧带不同分束断裂均可引起内侧半月板的退变,AMB断裂比PLB断裂更易引起内侧半月板退变。
[Abstract]:Objective to investigate the effect of (ACL) fragmentation of anterior cruciate ligament on medial meniscus in rabbits. Methods 36 New Zealand white rabbits were randomly divided into three groups: ACL anterior medial bundle (AMB) fracture group, posterolateral (PLB) fracture group and non-rupture group, with 12 rabbits in each group. The models of AMB rupture, PLB rupture and non-rupture of right hindlimb were established respectively. The knee joint function score of the surgical side was recorded at 4, 6 and 8 weeks, and the meniscus of the operation side was stained with hematoxylin and eosin. The expression of matrix protease 13 (MMP-13) in meniscus was detected by immunohistochemistry. Results at 4, 6 and 8 weeks after operation, the collagen fibers in the meniscus of the rabbits were normal in morphology, no vascular proliferation, no inflammatory cells, and the collagen fiber bundles were thick and arranged neatly. The surface of meniscus in AMB fracture group and PLB fracture group was gradually uneven, loose tissue, disordered arrangement of collagen fibers and infiltration of inflammatory cells. At 4, 6 and 8 weeks after operation, there was no significant difference in knee joint function score between the non-broken group and the non-ruptured group (P 0.05). The knee function scores of ABM fracture group and PLB fracture group were lower than 4 weeks after operation at 8 weeks (P 0.05), and the knee joint function scores at 8 weeks were lower than 6 weeks (P 0.05). At the same time, the functional scores of knee joint in AMB fracture group and PLB fracture group were significantly lower than those in non-rupture group (P 0.05). There was no significant difference in knee joint function score between AMB fracture group and PLB fracture group at the same time point (P 0.05). There was no significant difference in the percentage of MMP-13 positive cells in meniscus at 4, 6 and 8 weeks after operation in the unruptured group (P 0.05). The percentage of MMP-13 positive cells in the AMB fracture group and the PLB fracture group at 6 weeks was higher than that at 4 weeks after operation (P 0.05). The percentage of MMP-13 positive cells at 8 weeks was higher than that at 6 weeks (P 0.05). At the same time point, the percentage of MMP-13 positive cells in PLB fracture group was higher than that in unbroken group (P 0.05), and the percentage of MMP-13 positive cells in AMB fracture group was higher than that in PLB fracture group at the same time point (P 0.05). Conclusion the degeneration of medial meniscus can be caused by different bundles of anterior cruciate ligament in rabbits. AMB fracture is more likely to cause medial meniscus degeneration than PLB rupture.
【作者单位】：桂林医学院附属医院脊柱骨病外科;
【基金】：广西壮族自治区卫生厅基础研究计划课题(编号:Z2013468)
【分类号】：R684

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