内质网跨膜蛋白肌醇需求酶1α介导的凋亡通路在兔骨关节炎发病中的作用及机制

发布时间：2019-06-01 10:54

【摘要】：目的探讨内质网跨膜蛋白肌醇需求酶(IRE)1α介导的凋亡通路在骨关节炎(OA)发病中的作用及机制。方法新西兰兔膝关节注射木瓜蛋白酶模拟OA模型,HE染色检测OA关节软骨以及关节滑膜的形态,ELISA法检测OA关节滑膜中炎性因子白细胞介素(IL)-6的含量。检测闭合性松解术关节软骨的组织形态以及IL-6的含量。Western印迹检测IRE1α介导的凋亡通路在OA滑膜中的表达水平。结果 HE染色结果显示木瓜蛋白酶诱导的OA模型中,关节软骨细胞发生明显坏死,纤维组织变性、机化以及纤维蛋白坏死物沉积,ELISA结果显示关节滑膜中的IL-6水平明显升高,闭合性松解术后的OA关节软骨细胞凋亡减少,且关节滑膜中IL-6水平也明显降低。蛋白水平显示OA膝关节滑膜中IRE1α的磷酸化水平以及Caspase3的表达水平明显升高,介导关节软骨细胞的凋亡,而闭合性松解术后Caspase3的表达水平降低。结论闭合性松解术可缓解OA的症状,抑制IRE1α-Caspase3信号通路介导的凋亡作用。
[Abstract]:Objective to investigate the role and mechanism of apoptosis pathway mediated by endoplasmic reticulum transmembrane protein inositol demand enzyme (IRE) 1 伪 in the pathogenesis of osteoarthritis (OA). Methods New Zealand rabbits were injected with papain to simulate OA model. The morphology of cartilage and synovium of OA joint was detected by HE staining, and the content of inflammatory factor (IL)-6 in synovium of OA joint was detected by ELISA method. The tissue morphology and IL-6 content of articular cartilage after closed loosening were detected, and the expression of IRE1 伪 mediated apoptosis pathway in OA synovium was detected by Western blot. Results the results of HE staining showed that in the OA model induced by papain, the articular cartilage cells were obviously necrotic, fibrous tissue denaturation, mechanization and fibrin necrosis were deposited. ELISA results showed that the level of IL-6 in synovium of joint was significantly increased. After closed loosening, the apoptosis of cartilage cells in OA joint decreased, and the level of IL-6 in synovium also decreased significantly. The protein level showed that the phosphorylation level of IRE1 伪 and the expression of Caspase3 in synovial membrane of OA knee joint were significantly increased, which mediated the apoptosis of articular cartilage cells, while the expression level of Caspase3 decreased after closed loosening. Conclusion closed loosening can relieve the symptoms of OA and inhibit the apoptosis mediated by IRE1 伪-Caspase3 signaling pathway.
【作者单位】：黑龙江中医药大学附属第二医院骨科;
【基金】：黑龙江中医药大学科研基金项目(201310)
【分类号】：R684.3

【相似文献】