光交联透明质酸水凝胶结合包裹虫草素壳聚糖治疗骨关节炎

发布时间：2019-06-05 19:51

【摘要】：目的:本研究旨在研究通过使用虫草素壳聚糖微球(CM-虫草素)和光交联的透明质酸甲基丙烯酸酯水凝胶(HAMA)来促进软骨细胞自噬,评价此方法治疗骨关节炎的效果。方法:首先,我们开发和评价了 HAMA水凝胶和壳聚糖微球的特性。接下来,我们测量了在由白细胞介素-1β(IL-1β)诱导的软骨细胞和离体模型中虫草素对软骨基质降解的作用。我们发现虫草素可以通过自噬的激活保护软骨免于部分降解。此外,我们通过前交叉韧带切割手术诱导小鼠骨关节炎(OA),并在其关节腔内注射CM-虫草素和HAMA。通过组织学、免疫组织化学等评价CM-虫草素和HAMA水凝胶的组合对手术诱导的OA的疗效。结果:我们的研究结果表明:第一,虫草素能改善离体模型中的软骨退变;第二,虫草素能增强小鼠软骨细胞和人骨关节炎软骨的自噬;第三,壳聚糖虫草素和HAMA水凝胶的组合能减缓骨关节炎中退行的进展。结论:CM-虫草素和光交联的HAMA水凝胶的组合能明显改善手术诱导的骨关节炎的进展。
[Abstract]:Aim: to study the effect of cordycepin chitosan microspheres (CM- cordycepin) and photocrosslinked hyaluronic acid methacrylate hydrogel (HAMA) on cartilage autophagy and evaluate the effect of this method in the treatment of osteoarthritis. Methods: firstly, we developed and evaluated the properties of HAMA hydrogel and chitosan microspheres. Next, we measured the effect of cordycepin on cartilage matrix degradation in cartilage cells and in vitro models induced by IL-1 尾 (IL-1 尾). We found that cordycepin protects cartilage from partial degradation through autophagy activation. In addition, we induced osteoarthritis (OA), in mice by anterior cruciate ligament cutting and injected CM- cordycepin and HAMA. into their joint cavity. The efficacy of the combination of CM- cordycepin and HAMA hydrogel on surgically induced OA was evaluated by histology and immunohistochemistry. Results: our results showed that: first, cordycepin could improve cartilage degeneration in vitro model, second, cordycepin could enhance autophagy of mouse cartilage cells and human osteoarthritis cartilage. Third, the combination of chitosan cordycepin and HAMA hydrogel can slow down the progress of osteoarthritis. Conclusion: the combination of CM- cordycepin and photocrosslinked HAMA hydrogel can significantly improve the progress of surgery-induced osteoarthritis.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R684.3
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本文编号：2493782