含“界层结构”支架复合自体BMSCs修复猪膝关节骨软骨损伤实验研究

发布时间：2019-06-08 11:06

【摘要】：目的:探索含“界层结构”仿生一体化骨软骨支架复合自体骨髓间充质干细胞(BMSCs)修复猪膝关节骨软骨缺损的有效性。方法:选用9只小香猪,同一实验动物一侧膝关节股骨滑车面分别造含实验组及无阴性对照组骨软骨缺损模型,共6只。随机将实验动物分为:含“界层结构”nano-HA/CPPf/PLLA仿生一体化支架+自体BMSCs的实验组(6只12膝);无“界层结构”CPPf/PLLA支架的阴性对照组(6只12膝);不做任何处理的单纯关节骨软骨缺损为空白对照组(3只3膝)。于术后12周、24周进行实验标本大体观察、组织学观察、MRI检测及组织学评分。结果:大体观察、组织学观察及MRI检测示实验组修复趋势较阴性对照组和空白对照组明显,但是三组均未能完全修复骨软骨缺损。组织学观察三组软骨修复区均以纤维样组织修复为主,软骨修复区仅可见少量软骨细胞或无软骨细胞出现,软骨下骨区则未见成骨细胞出现。以上三组于术后12周、24周行Pineda组织学评分,评分结果进行统计分析,组间和组内不同时间点比较差异无统计学意义(P0.05)。结论:含“界层结构”nano-HA/CPPf/PLLA仿生一体化支架复合自体骨髓间充质干细胞未能明显修复猪膝关节骨软骨缺损,修复组织主要为纤维组织。
[Abstract]:Aim: to explore the effectiveness of biomimetic integrated osteochondral scaffold containing "boundary layer structure" combined with autologous bone marrow mesenchymal stem cells (BMSCs) in repairing osteochondral defects of pig knee joint. Methods: nine Xiaoxiang pigs were selected to establish osteochondral defect models with experimental group and control group with and without negative osteochondral defects on one side of the knee joint of the same experimental animal. The experimental animals were randomly divided into experimental group (n = 6, 12 knees) containing "boundary layer structure" nano-HA/CPPf/PLLA biomimetic integrated stent autologous BMSCs, negative control group without "boundary layer structure" CPPf/PLLA stent (6 cases, 12 knees), and negative control group (6 cases, 12 knees). The simple articular cartilage defects without any treatment were blank control group (3 knees). At 12 weeks and 24 weeks after operation, the gross observation, histology observation, MRI detection and histology score of the experimental specimens were carried out. Results: gross observation, histologic observation and MRI test showed that the repair trend of the experimental group was more obvious than that of the negative control group and the blank control group, but none of the three groups could completely repair the osteochondral defects. Histologically, the cartilage repair area of the three groups was mainly fibroid tissue repair, only a small number of cartilage cells or no cartilage cells were found in the cartilage repair area, and no osteoblasts were found in the subcartilage bone area. The above three groups were treated with Pineda histology score at 12 weeks and 24 weeks after operation, and the results were statistically analyzed. There was no significant difference between the three groups at different time points (P 0.05). Conclusion: the "boundary layer structure" nano-HA/CPPf/PLLA biomimetic integrated scaffold combined with autologous bone marrow mesenchymal stem cells can not significantly repair the osteochondral defects of pig knee joint, and the repair tissue is mainly fiber tissue.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R684.3

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