乌司他丁对光损伤大鼠视网膜的保护作用

发布时间：2017-12-26 23:07

本文关键词：乌司他丁对光损伤大鼠视网膜的保护作用　出处：《中国临床研究》2016年12期 　论文类型：期刊论文

【摘要】：目的探讨乌司他丁对大鼠视网膜紫外线光损伤的保护作用及其机制。方法将36只SD大鼠随机分为对照组、光损伤组和给药组,每组各12只计24只眼。采用光照强度为(2 200±138)Lux的300~400 nm紫外线间歇照射法制作视网膜损伤模型。光照前3 d起,给药组腹腔单剂量注射乌司他丁2×104U·kg-1·d-1,其他组给予等量生理盐水腹腔注射至取标本日。分别于光损伤后3 d、7 d处死大鼠,摘出眼球。HE染色后观察大鼠视网膜的组织学变化,免疫组织化学方法检测B细胞淋巴瘤/白血病-2蛋白(Bcl-2)和Bcl-2相关X蛋白(Bax)在视网膜的相对表达量。结果对照组大鼠视网膜结构完整,层次清楚;光损伤组的视网膜结构紊乱,且随时间延长紊乱程度明显加重;给药组大鼠视网膜形态较对照组稍微紊乱,但紊乱程度均轻于相同时间段光损伤组。光损伤组大鼠的视网膜外核层(outer nuclear layer,ONL)厚度均低于同时间段对照组(P0.05,P0.01);给药组大鼠的各时间段视网膜ONL厚度均高于同时间段光损伤组(P0.05,P0.01),但低于正常对照组(P均0.01)。光损伤组中Bax蛋白在视网膜的相对表达量明显高于对照组,给药组中,Bax在视网膜的表达也较对照组明显增强,但明显弱于相应时间段光损伤组(P均0.05)。光损伤组的Bcl-2蛋白在视网膜的相对表达量明显低于对照组(P0.05),给药组Bcl-2蛋白相对表达量较对照组明显降低(P0.05),但较相应时间段光损伤组的相对表达量明显增加(P0.05)。结论乌司他丁对紫外线照射造成的视网膜损伤具有保护作用,其机制可能与乌司他丁抑制Bax蛋白表达而促进Bcl-2蛋白表达从而抑制紫外线诱导的凋亡有关。
[Abstract]:Objective to investigate the protective effect of ulinastatin on ultraviolet light damage in the retina of rats and its mechanism. Methods 36 SD rats were randomly divided into the control group, the light injury group and the drug delivery group, with 12 rats in each group with 24 eyes. The retinal damage model was made by using the light intensity of (2200 + 138) Lux 300~400 nm ultraviolet intermittent irradiation. A single dose of ulinastatin was injected into the intraperitoneal dose of 2 * 104U / kg-1 / D-1 in the administration group, and the other groups were given equal amount of saline injection to the day of sample 3 D. The rats were killed at 3 D and 7 d after light injury, and their eyeballs were removed. HE staining was used to observe histological changes of rat retina. Immunohistochemistry was used to detect the relative expression of B cell lymphoma / leukemia -2 protein (Bcl-2) and Bcl-2 related X protein (Bax) in the retina. Results in the control group, the retinal structure was complete and clear in layers. The retinal structure in light injury group was disorder, and the degree of derangement increased significantly with time. The retinal morphology in the drug treated group was slightly more disorder than that in the control group, but the degree of derangement was lighter than that in the light injury group at the same time. The thickness of outer nuclear layer (ONL) in the light injury group was lower than that in the same time control group (P0.05, P0.01). The thickness of retinal ONL in each time group was higher than that in the light injury group (P0.05, P0.01) at the same time, but it was lower than that in the normal control group (P all 0.01). In the light injury group, the relative expression of Bax protein in the retina was significantly higher than that in the control group. In the drug delivery group, the expression of Bax in the retina was also significantly higher than that in the control group, but it was significantly weaker than that in the corresponding time period light damage group (P 0.05). The relative expression of Bcl-2 protein in the retinal injury group was significantly lower than that in the control group (P0.05). The relative expression of Bcl-2 protein in the treatment group was significantly lower than that in the control group (P0.05), but the relative expression level increased significantly compared with that in the corresponding time group (P0.05). Conclusion Ulinastatin has protective effect on retinal injury induced by ultraviolet radiation, and its mechanism may be related to Ulinastatin inhibiting the expression of Bax protein and promoting the expression of Bcl-2 protein, thus inhibiting ultraviolet induced apoptosis.
【作者单位】：湘南学院基础医学院;河北联合大学附属医院护理部;郴州市第一人民医院眼科;湘南学院药学院;
【基金】：湖南省教育厅资助科研项目(12C0893) 湖南省重点建设学科资金资助[湘教发(2011)76号] 湖南省高校重点实验室资金资助[湘教通(2008)246号]
【分类号】：R774.1
【正文快照】： 视网膜光损伤主要包括光的热损伤、光的机械损伤和光的化学损伤[1]。光的化学损伤被认为在视网膜光损伤中具有重要作用,并与老年性黄斑变性、手术后视网膜损伤等疾病密切相关[2],其发病机制可能涉及自由基和脂质过氧化反应[3-4]、细胞内钙负荷过重[5]、细胞色素氧化酶抑制[6]

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