Toll样受体2调节Th细胞极化对小鼠角膜移植排斥反应发生的影响

发布时间：2017-12-26 23:13

本文关键词：Toll样受体2调节Th细胞极化对小鼠角膜移植排斥反应发生的影响　出处：《眼科新进展》2016年07期 　论文类型：期刊论文

更多相关文章： Toll样受体 角膜移植 免疫排斥 Th Th Th

【摘要】：目的探究Toll样受体2(Toll like receptor 2,TLR2)是否通过调节辅助性T细胞(T helper cells,Th)极化影响角膜移植排斥反应的发生。方法取30只C57BL/6小鼠和30只TLR2基因敲除小鼠为受体,BALB/c小鼠为供体,在受体小鼠右眼行同种异体角膜移植术,分别为野生组和基因敲除组;取19只C57BL/6小鼠右眼行自体角膜移植术,为自体组。术后每周两次裂隙灯下观察植片水肿程度和透明度,参照Sonoda评分标准对排斥指数(reject index,RI)进行评分;术后14 d收集角膜。分别取9只C57BL/6小鼠和TLR2基因敲除小鼠作为野生对照组和基因敲除对照组,行常规HE染色和实时荧光定量PCR检测。结果术后随时间变化各手术组植片水肿和混浊程度不一,以野生组最为严重。角膜RI评分显示术后7 d、14 d、21 d,基因敲除组(0.80±0.83、0.90±0.55、1.35±0.99)低于野生组(1.55±0.94、2.25±0.97、2.55±1.19),差异均有统计学意义(P=0.008、0.000、0.000)。HE染色显示,野生组角膜基质层出现水肿和大量炎性细胞;而基因敲除组和自体组炎症反应较轻。PCR检测显示,基因敲除组角膜Th1和Th17细胞因子(IFN-γ和IL~(-1)7)mRNA表达(1.94±0.34、2.62±0.30)比野生组(4.27±0.37、3.99±0.40)低,差异均有统计学意义(P=0.000、0.001);Th2细胞因子(IL-4)三组差异无统计学意义(P0.05)。结论敲除TLR2可能通过抑制Th向Th1和Th17极化,降低角膜移植排斥率。
[Abstract]:Objective to investigate whether Toll like receptor 2 (Toll like receptor 2, TLR2) influences the occurrence of corneal allograft rejection by regulating the polarization of helper T cells (T helper cells, Th). Methods 30 C57BL/6 mice and 30 TLR2 knockout mice were taken as recipients, BALB/c mice as donors, and allogenic keratoplasty in the right eyes of recipient mice, respectively, in wild group and gene knockout group. 19 C57BL/6 mice in right eye underwent autologous corneal transplantation. The degree of edema and transparency of the graft were observed two times a week after slit lamp. The reject index (RI) score was evaluated according to the Sonoda score, and the corneas were collected at 14 d after operation. 9 C57BL/6 mice and TLR2 gene knockout mice were taken as the wild control group and the gene knockout control group, and the routine HE staining and real-time fluorescence quantitative PCR detection were performed. Results after the operation, the edema and turbidity were not one of the surgical groups, and the most serious in the wild group. The corneal RI score was 7 d, 14 d, 21 d after operation, and the knockout group (0.80 + 0.83, 0.90 + 0.55, 1.35 + 0.99) was lower than that in the wild group (1.55, 0.94, 0.94, and 1.55). HE staining showed edema and a large number of inflammatory cells in the stroma layer of the wild group, while the inflammatory reaction in the gene knockout group and the autologous group was mild. PCR analysis showed that the gene knockout group of corneal Th1 and Th17 cytokines (IFN- gamma and IL~ (-1) 7) the expression of mRNA (1.94 + 0.34, 2.62 + 0.30) than the wild group (4.27 + 0.37, 3.99 + 0.40) is low, the differences were statistically significant (P=0.000, 0.001) (IL-4; Th2 cell factor) there was no significant difference between the three groups (P0.05). Conclusion knockout TLR2 may reduce the rejection rate of corneal transplantation by inhibiting the polarization of Th to Th1 and Th17.
【作者单位】：南方医科大学南方医院眼科;
【基金】：国家自然科学基金资助(编号:81170887) 南方医科大学南方医院横向课题基金资助(编号:G201202)~~
【分类号】：R779.65
【正文快照】： l l 孟婷出生,广东佛山人,在读硕士研究生。研究方向:角膜移植免疫。联系电话:15622138590;E-mail:xha_000@163.com;ORCID:0000-0001-8327-3140About SU Ya-Ru:Female,born inMay,1991.Master degree.Tel:15622138590;E-mail:xha_000@163.com;ORCID:0000-0001-8327-3140修回日

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