联合EBNA和Bamh1-W的实时PCR方法检测EB病毒DNA在鼻咽癌中的应用

发布时间：2018-02-19 22:33

本文关键词： EB病毒 EBNA Bamh-W 实时荧光定量PCR 鼻咽癌　出处：《实用医学杂志》2017年17期 　论文类型：期刊论文

【摘要】：目的探讨基于EB病毒EBNA基因设计引物的实时PCR在EB病毒DNA检测中的应用以及联合常用方法(Bamh1-W片段)检测在鼻咽癌患者中的诊断价值。方法用两种方法对234例血液标本(包括66例鼻咽癌患者标本)进行EB病毒DNA定量检测,每例样本对细胞内及细胞外分别测定,比较两种方法以及联合检测在各组中的阳性率,观察EBNA法联合Bamh1-W法在鼻咽癌患者中对EB病毒检出率有无提高,方法学的验证采用回归分析及t检验。结果 EBNA法的阳性率(所有样本53.42%,鼻咽癌样本51.52%)低于Bamh1-W法(所有样本69.23%,鼻咽癌样本71.21%),但联合两种方法检测可提高阳性率(所有样本70.94%,鼻咽癌样本72.73%)且在鼻咽癌患者中得到了更大的提高,EBNA法与Bamh1-W法检测标本的相关性R2为0.577,且P0.05,差异有统计学意义,但在鼻咽癌样本中R2为0.828,相关性较好且P0.05,差异无统计学意义。结论联合EBNA法和Bamh1-W法检测EB病毒DNA可提高阳性率且在鼻咽癌患者的检测中阳性率能够得到更大的提高,对EB病毒DNA定量和鼻咽癌的辅助诊断具有一定的意义。
[Abstract]:Objective to investigate the application of real-time PCR based on EB virus EBNA gene design primer in EB virus DNA detection and the diagnostic value of the combined method of Bamh1-W fragment in patients with nasopharyngeal carcinoma. Methods two methods were used in 234 patients with nasopharyngeal carcinoma. Epstein-Barr virus (EBV) DNA was quantitatively detected in 66 patients with nasopharyngeal carcinoma (NPC). The positive rates of EBV in each group were compared between the two methods and the combined method, and the positive rate of EBV was observed by EBNA combined with Bamh1-W in patients with nasopharyngeal carcinoma (NPC). Results the positive rate of EBNA (all samples 53.42, NPC 51.522) was lower than that of Bamh1-W (all samples 69.23, nasopharyngeal carcinoma 71.21), but the combination of two methods could improve the positive rate (P < 0.05). All samples 70.94 and nasopharyngeal carcinoma 72.73) and the correlation between EBNA and Bamh1-W was 0.577, and P0.05, the difference was statistically significant. But the R2 of NPC samples was 0.828, the correlation was good and the difference was not statistically significant (P 0.05). Conclusion the positive rate of EBV DNA detected by combined EBNA and Bamh1-W can be increased and the positive rate of EBV DNA can be increased in NPC patients. It has certain significance for EB virus DNA quantification and auxiliary diagnosis of nasopharyngeal carcinoma.
【作者单位】：华中科技大学同济医学院附属协和医院检验科;华中科技大学同济医学院附属协和医院肿瘤中心;
【基金】：国家自然科学基金项目(编号:81374235)
【分类号】：R730.43;R739.63

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