AEG-1和LEF-1在眼附属器MALT淋巴瘤的表达及意义

发布时间：2018-02-21 04:25

本文关键词： 眼附属器淋巴瘤 AEG-1 LEF-1 MALT　出处：《青岛大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:研究AEG-1(astrocyte elevated gene-1)、LEF-1(即淋巴细胞增强因子)在眼附属器粘膜相关组织淋巴瘤(marginal zone lymphoma of mucosa-associated lymphoid tissue type,MALT)和反应性淋巴组织增生的表达,从蛋白和基因水平探讨AEG-1和LEF-1的表达与眼附属器MALT淋巴瘤发生发展关系,其蛋白表达与临床病理特征的关系和两者蛋白表达的相互关系及可能的机制。方法:搜集1995年1月到2015年1月青岛大学附属医院眼科病理实验室眼附属器MALT淋巴瘤石蜡包埋标本和冻存组织,其中40例石蜡标本、8例冻存组织为实验组,对照组为反应性淋巴组织增生,其中石蜡标本20例、冻存组织8例。应用免疫组织化学染色法检测AEG-1和LEF-1蛋白水平的表达,应用实时荧光定量PCR法检测实验组和对照组中AEG-1和LEF-1m RNA水平的表达,统计学分析采用SPSS17.0。结果:1.AEG-1的蛋白表达及m RNA含量:a.AEG-1阳性颗粒主要定位在细胞胞膜上,少量在胞浆内(图3)。b.眼附属器MALT淋巴瘤中AEG-1的阳性表达率(62.5%)显著高于反应性淋巴组织增生(P0.05,表1)。c.AEG-1的阳性表达与眼附属器MALT淋巴瘤的临床分期有关(P0.05,表2),随着临床分期的增加,其表达增加,而与性别、年龄、肿瘤发生部位无关。d.AEG-1 m RNA在眼附属器MALT淋巴瘤中显著高于反应性淋巴组织增生(P0.05,图9)。2.LEF-1的蛋白表达及m RNA含量:a.LEF-1阳性颗粒主要定位在细胞胞核内(图6)。b.眼附属器MALT淋巴瘤中LEF-1的阳性表达率(65%)显著高于反应性淋巴组织增生(P0.05,表3)。c.LEF-1的阳性表达与眼附属器MALT淋巴瘤的临床分期有关(P0.05,表4),随着临床分期的增加,其表达增加,而与患者性别、年龄、肿瘤发生部位无关。d.AEG-1 m RNA在眼附属器MALT淋巴瘤中显著高于反应性淋巴组织增生(P0.05,图10)。3.AEG-1和LEF-1蛋白表达与眼附属器MALT淋巴瘤的关系:AEG-1和LEF-1在眼附属器MALT组织中表达增高,阳性表达率分别为62.5%、65%,两者之间存在正相关(P0.05,表5)Spearman相关系数为r=0.406。结论:AEG-1和LEF-1的表达增高可能参与眼附属器MALT淋巴瘤的发生发展,两者表达成正相关,可能通过某种模式在眼附属器MALT中发挥作用。两种因子可能成为眼附属器MALT淋巴瘤的诊断标记物,为眼附属器淋巴瘤病理诊断提供依据。
[Abstract]:Objective: to study the expression of AEG-1(astrocyte elevated gene-1 lef 1 (Lymphocyte Enhancement Factor) in peripheral zone lymphoma of mucosa-associated lymphoid tissue type malt and reactive lymphoid tissue hyperplasia. To explore the relationship between the expression of AEG-1 and LEF-1 and the occurrence and development of ocular accessory MALT lymphoma from the protein and gene levels. The relationship between protein expression and clinicopathological features, the relationship between protein expression and the possible mechanism were analyzed. Methods: from January 1995 to January 2015, MALT lymph nodes were collected in ophthalmic pathology laboratory of Qingdao University Hospital. Paraffin embedded specimens and frozen tissue, The expression of AEG-1 and LEF-1 protein was detected by immunohistochemical staining method in 40 paraffin specimens and 8 frozen tissues, including 20 paraffin wax specimens and 8 frozen tissues. The expression of AEG-1 and LEF-1m RNA in the experimental group and the control group were detected by real-time fluorescence quantitative PCR method. SPSS 17.0. the results showed that the protein expression of 1 / 1 AEG-1 and the content of m RNA were mainly located on the cell membrane. The positive expression rate of AEG-1 in cytoplasm (Fig. 3n.b. ocular accessory MALT lymphoma) was significantly higher than that in reactive lymphoid tissue hyperplasia (P0.05. Table 1. C. AEG-1 was associated with the clinical staging of MALT lymphoma of ocular appendage, P 0.05, Table 2, with the increase of clinical stage. Its expression increases with sex, age, There was no correlation between tumor location and tumor location. D. AEG-1 m RNA was significantly higher in ocular adnexal MALT lymphoma than in reactive lymphoid tissue hyperplasia (P0.05). Fig. 9. 2. The protein expression of LEF-1 and the content of m RNA:: a.LEF-1 were mainly located in the cellular nucleus (Fig. 6). The positive expression rate of LEF-1 in lymphoma was significantly higher than that in reactive lymphoproliferative tissue hyperplasia (P 0.05). The positive expression of LEF-1 was related to the clinical stage of ocular adnexal MALT lymphoma (P 0.05, Table 4), and with the increase of clinical stage, the positive expression of Lymphoma was significantly higher than that of reactive lymphoid tissue hyperplasia (P 0.05). Its expression increased with the patient's gender, age, The expression of .d.AEG-1 m RNA in ocular adnexal MALT lymphoma was significantly higher than that in reactive lymphoid tissue hyperplasia (P0.05). Fig. 10. 3. The relationship between the expression of AEG-1, AEG-1 and LEF-1 protein and the expression of LEF-1 in ocular adnexal MALT lymphoma was significantly higher than that in ocular adnexal MALT lymphoma. The positive expression rates were 62.5% and 65%, respectively. There was a positive correlation between them (P 0.05), and the correlation coefficient between them was 0.406.Conclusion the increased expression of LEF-1 and AEG-1 may be involved in the occurrence and development of ocular appendage MALT lymphoma, and there is a positive correlation between them. These two factors may be used as diagnostic markers of ocular accessory MALT lymphoma and provide basis for pathological diagnosis of ocular adnexal lymphoma.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R739.7

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