RTN4基因rs2864052和rs6545468与广西壮族人群鼻咽癌易感性相关研究

发布时间：2018-02-27 07:16

本文关键词： 鼻咽肿瘤 RTN基因多态性单倍型　出处：《临床耳鼻咽喉头颈外科杂志》2016年24期 　论文类型：期刊论文

【摘要】：目的:研究RTN4多态性rs2864052和rs6545468位点及其单倍型与广西壮族人群鼻咽癌易感性相关关系。方法:运用单碱基延伸PCR技术和DNA测序法对广西壮族282例鼻咽癌患者(鼻咽癌组)和199例健康体检者(对照组)的RTN4基因rs2864052和rs6545468位点进行基因分型,并分析RTN4基因的单倍型频率。结果:RTN4基因rs2864052存在AA、GA、GG三种基因型和A、G等位基因,其在鼻咽癌组与对照组中的基因型、等位基因频率的分布差异无统计学意义;rs6545468位点存在CC、GC、GG三种基因型和C、G等位基因,其在鼻咽癌组与对照组中的基因型、等位基因频率的分布差异亦无统计学意义。但单倍型分析发现鼻咽癌组单倍型AG频率显著低于对照组(P=0.004,OR=0.14,95%CI为0.31-0.68)。结论:RTN4基因多态性rs2864052和rs6545468位点的单倍型AG可降低广西壮族人群个体鼻咽癌易感性。
[Abstract]:Objective: to study the association of RTN4 polymorphism rs2864052 and rs6545468 loci and their haplotypes with susceptibility to nasopharyngeal carcinoma (NPC) in Guangxi Zhuang nationality. Methods: single base extension PCR technique and DNA sequencing were used in 282 patients with nasopharyngeal carcinoma (NPC) in Guangxi Zhuang nationality. The rs2864052 and rs6545468 loci of RTN4 gene were genotyped in 199 healthy persons (control group) and cancer group (n = 199). The frequency of haplotype of RTN4 gene was analyzed. Results there were three genotypes and alleles in rs2864052 of RTN4 gene, which were genotypes in nasopharyngeal carcinoma (NPC) group and control group. There was no significant difference in allelic frequency distribution at rs6545468. There were three genotypes of CCG GCU GG and C G alleles, which were genotypes in nasopharyngeal carcinoma (NPC) group and control group (P < 0.05). But haplotype analysis showed that the AG frequency of haplotype in nasopharyngeal carcinoma group was significantly lower than that in control group. The CI was 0.31-0.68. Conclusion the haplotype AG at the rs2864052 and rs6545468 loci of the gene polymorphism can be decreased in Guangxi. The susceptibility of individual nasopharyngeal carcinoma in Zhuang nationality.
【作者单位】：右江民族医学院附属医院检验科;
【基金】：国家自然科学基金(No:81560461)
【分类号】：R739.63

【相似文献】