美登木素抑制鼻咽癌的增殖以及侵袭迁移机制的生物信息学分析

发布时间：2018-02-27 12:39

本文关键词： 鼻咽肿瘤美登木素增殖侵袭迁移生物信息学　出处：《实用医学杂志》2017年08期 　论文类型：期刊论文

【摘要】：目的:初步验证美登木素对鼻咽癌细胞株增殖以及侵袭迁移的抑制作用,利用公共数据库芯片分析美登木素对细胞株增殖以及侵袭迁移的作用机制。方法:体外实验检测美登木素对鼻咽癌细胞株增殖、侵袭迁移的影响。生物信息学筛选差异表达基因、功能富集分析并进一步筛选出核心基因。分析上调、下调基因的蛋白网络(PPI),并计算蛋白网络以及功能富集分析的核心模块。结果:美登木素显著抑制鼻咽癌细胞的增殖以及侵袭迁移,生物信息学分析得到了美登木素可能影响鼻咽癌的291个上调差异基因,81个下调差异基因。PPI分析表明8个上调的差异表达基因具有显著的生物学意义。结论:美登木素对鼻咽癌细胞株的增殖、侵袭以及迁移具有明显的抑制作用,生物信息学表明CDKN1B、BMP2、HBEGF、SMAD6、ADM、VEGFA、CTGF、IL6等基因的异常表达可能在美登木素对鼻咽癌的侵袭、迁移的抑制中起重要作用。
[Abstract]:Objective: to investigate the inhibitory effect of medenin on the proliferation, invasion and migration of nasopharyngeal carcinoma cell lines. The mechanism of Madden lignin on proliferation, invasion and migration of nasopharyngeal carcinoma cell line was analyzed by common database chip. Methods: in vitro, the proliferation of nasopharyngeal carcinoma cell line was detected. Bioinformatics screening of differentially expressed genes, functional enrichment analysis and further screening of core genes. The protein network down-regulated the protein network of the gene and calculated the core modules of protein network and function enrichment analysis. Results: Meiden lignin significantly inhibited the proliferation and invasion and migration of nasopharyngeal carcinoma cells. Bioinformatics analysis showed that Madden lignin might affect 291 up-regulated differentially expressed genes in nasopharyngeal carcinoma, 81 down-regulated differentially expressed genes. PPI analysis showed that 8 up-regulated differentially expressed genes had significant biological significance. Proliferation of nasopharyngeal carcinoma cell lines induced by lignin, Invasion and migration were inhibited obviously. Bioinformatics indicated that the abnormal expression of CDKN1BMP2 / HBEGFSMAD6 / VEGFAA CTGFGF-IL6 may play an important role in the inhibition of invasion and migration of nasopharyngeal carcinoma induced by Madden lignin.
【作者单位】：南方医科大学基础医学院肿瘤研究所;
【基金】：国家高技术研究发展计划(863计划)项目(编号:2012AA020603-03)
【分类号】：R739.63

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