鼻咽癌裸鼠移植瘤组织的ADC值与HIF-1α的相关性研究

发布时间：2018-03-03 19:26

本文选题：乏氧诱导因子　切入点：ADC值　出处：《福建医科大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的:检测乏氧诱导因子（HIF-1α）在荷人鼻咽癌裸鼠移植瘤组织不同水平的表达及分析移植瘤组织MRI表观弥散系数（ADC）值照射前后的动态变化并探讨二者的相关性。方法：用免疫功能缺陷型的Balb/c裸鼠建立人鼻咽癌CNEII细胞移植瘤模型，待瘤组织平均最大直径达1.0cm左右时，将36只裸鼠随机分成6组（每组6只）。对照组：不做任何处理；照射组给予6MV-X线，单次15Gy照射根据照射后的时间分为第1天组、第2天组、第3天组、第5天组、第7天组，MRI测量照射前、后移植瘤组织ADC值大小，采用MaxVision免疫组织化学方法检测移植瘤组织照射前、后HIF-1α的表达情况；采用Real-time PCR和Western Blot方法检测移植瘤组织照射前、后HIF-1α在mRNA和蛋白水平的表达水平。比较照射前、后的ADC值的变化情况及其与HIF-1α在不同水平表达的相关性。结果：（1）HIF-1α在裸鼠移植瘤组织中有较高的表达，阳性率为69.4%。（2）照射后HIF-1αmRNA表达水平第2、3天较照射前升高，有显著统计学意义，（P＜0.01）照射后第7天较照射前降低；有显著统计学意义，（P＜0.01）。（3）照射后HIF-1α蛋白表达水平第2天较照射前升高，有显著统计学意义，（P＜0.01）,照射后第5、7天较照射前降低；有显著统计学意义，（P＜0.05）。（4）照射后ADC值第2天较照射前降低，照射后第7天较照射前升高，有显著统计学意义，（P＜0.05）。（5）在整个标本中，HIF-1αmRNA表达水平与ADC值呈负相关，(rs=-0.727，P0.01)；HIF-1α蛋白表达水平与ADC值呈负相关(rs=-0．618，P0.01)，各对照组间无明显相关性。结论：荷人鼻咽癌裸鼠移植瘤组织中ADC值与HIF-1α的表达呈负相关，这可能为ADC值预测鼻咽癌放疗敏感性的机制之一，进一步研究ADC值预测鼻咽癌放疗敏感性的机制有待于进一步研究。
[Abstract]:Aim: to detect the expression of hypoxia inducible factor (HIF-1 伪) in nude mice transplanted with human nasopharyngeal carcinoma (NPC) at different levels, and to analyze the dynamic changes of MRI diffusion coefficient before and after irradiation, and to explore the correlation between HIF-1 伪 and HIF-1 伪. Methods: the transplanted tumor model of human nasopharyngeal carcinoma (NPC) CNEII cells was established in nude mice with immunodeficient Balb/c. When the average maximum diameter of tumor tissue was about 1.0 cm, 36 nude mice were randomly divided into 6 groups (6 in each group). 6MV-X ray was given to the irradiation group. According to the time after irradiation, single 15Gy irradiation was divided into the first day group, the second day group, the third day group, the fifth day group, the 7th day group, the ADC value of the transplanted tumor tissue before and after irradiation were measured. MaxVision immunohistochemical method was used to detect the expression of HIF-1 伪 before and after irradiation, and Real-time PCR and Western Blot methods were used to detect the expression of HIF-1 伪 in mRNA and protein levels before and after irradiation. The change of ADC value and its correlation with the expression of HIF-1 伪 at different levels. Results the expression of HIF-1 伪 HIF-1 伪 in transplanted tumor tissues of nude mice was higher than that before irradiation. The positive rate was 69.40.0.The expression level of HIF-1 伪 mRNA increased on the 2nd day after irradiation compared with that before irradiation (P < 0.01). The expression rate of HIF-1 伪 was significantly lower on the 7th day after irradiation than that before irradiation. The expression level of HIF-1 伪 protein increased significantly on the 2nd day after irradiation (P < 0.01), and decreased on the 5th day after irradiation (P < 0.05), and the ADC value decreased on the second day after irradiation as compared with that before irradiation (P < 0.01), and the expression level of HIF-1 伪 protein decreased significantly at the second day after irradiation (P < 0.01), and the expression level of HIF-1 伪 protein was significantly lower than that before irradiation on the second day after irradiation (P < 0.01), and was significantly lower on the 5th and 7th day after irradiation than that before irradiation (P < 0.05). On the 7th day after irradiation, the expression of HIF-1 伪 mRNA was significantly higher than that before irradiation (P < 0.05, P < 0.05). There was a negative correlation between the expression of HIF-1 伪 mRNA and ADC value. There was a negative correlation between the expression of HIF-1 伪 protein and ADC value. There was no significant correlation between HIF-1 伪 expression level and ADC value. Conclusion: there is a negative correlation between ADC and HIF-1 伪 expression in nude mice transplanted with human nasopharyngeal carcinoma, which may be one of the mechanisms by which ADC value can predict the radiosensitivity of nasopharyngeal carcinoma. Further study on the mechanism of ADC value in predicting radiosensitivity of nasopharyngeal carcinoma needs further study.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.63

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