胰岛素样生长因子-1抑制庆大霉素引起的小鼠耳蜗毛细胞凋亡

发布时间：2018-03-23 22:01

本文选题：胰岛素样生长因子-　切入点：Notch/hes信号通路　出处：《中国老年学杂志》2017年18期

【摘要】：目的探讨胰岛素样生长因子(IGF)-1是否通过Notch信号通路抑制庆大霉素(GM)引起的离体小鼠耳蜗毛细胞凋亡。方法体外培养新生小鼠耳蜗基底膜,在分别含有正常对照培养液(A组)、0.5 mmol/L GM(B组)、0.5 mmol/L GM+10 ng/ml IGF-1(C组)的成分中培养24 h后收集细胞及固定。应用TUNEL法观察各组耳蜗毛细胞的凋亡情况。应用Western印迹、实时定量聚合酶链反应(PCR)观察各组小鼠基底膜Notch2/hes1信号通路的表达量。结果 A组观察到少量凋亡毛细胞,B组耳蜗毛细胞凋亡数量及Notch2/hes1的表达量较A组明显上升(P0.01),C组细胞凋亡数量及Notch2/hes1的表达量较B组明显下降(P0.01)。结论 IGF-1通过抑制Notch2/hes1信号通路,减少GM诱导的离体耳蜗毛细胞凋亡。
[Abstract]:Objective to investigate whether insulin-like growth factor (IGFF-1) inhibits the apoptosis of mouse cochlear hair cells induced by gentamicin via Notch signaling pathway. Methods the basal membrane of newborn mouse cochlea was cultured in vitro. The cells were collected and fixed after 24 h culture in the components of 0.5 mmol/L GM 10 ng/ml IGF-1(C group containing 0.5 mmol/L GM(B group, respectively. The apoptosis of cochlear hair cells in each group was observed by TUNEL method. Western blotting was used to observe the apoptosis of cochlear hair cells in each group. Results A small amount of apoptotic hair cells and Notch2/hes1 expression in cochlea hair cells were observed in group A. the expression of Notch2/hes1 in cochlear hair cells in group A was significantly higher than that in group A (P 0.01). The number of apoptosis and the expression of Notch2/hes1 in group A were significantly lower than those in group B. conclusion IGF-1 can inhibit Notch2/hes1 signaling pathway. Reduce GM induced apoptosis of cochlear hair cells in vitro.
【作者单位】：辽宁医学院附属第一医院耳鼻喉科;
【基金】：2014年辽宁省教育厅科学研究项目(No.L2014323) 辽宁医学院校长基金-奥鸿博泽研究生科技创新基金(No.AH2014015)
【分类号】：R764

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