miRNA-29在糖尿病视网膜病变患者血清中的表达分析研究

发布时间：2018-04-01 12:13

  本文选题：糖尿病　切入点：视网膜病变　出处：《中国地方病防治杂志》2017年02期

【摘要】：目的研究糖尿病视网膜病变患者血清中miRNA-29的表达水平,分析并探讨miRNA-29在糖尿病视网膜病变发生机制中的作用。方法选取健康受检者、糖尿病患者及糖尿病伴有视网膜病变患者,对所参加实验者静脉取血,提取并纯化血浆总RNA,并通过逆转录聚合酶链反应等实验技术获取并检测miRNA-29的表达水平。结果糖尿病视网膜病变组患者和糖尿病不伴并发症患者相比miRNA-29的表达显著增高,差异具有统计学意义(P0.01),无并发症的糖尿病患者组和健康对照组比较,miRNA-29的表达无明显改变,差异无统计学意义(P0.05);糖尿病视网膜病变患者组和不伴并发症患者糖尿病患者组、健康对照组相比,RAX蛋白表达水平明显降低,差异具有统计学意义(P0.05)结论miRNA-29在糖尿病视网膜病变患者血清中表达明显上调,miRNA-29潜在靶基因PKR结合蛋白X(RAX)表达增高,其可能参与糖尿病视网膜病变的发生机制,进而引起糖尿病视网膜病变。
[Abstract]:Objective to study the expression level of serum miRNA-29 in diabetic retinopathy patients, analyze and discuss the role of miRNA-29 in the pathogenesis of diabetic retinopathy. Methods healthy subjects, patients with diabetes and diabetic retinopathy, to participate in the experiment were venous blood, extraction and purification of total plasma RNA expression level, and through the experimental technique of reverse transcriptase polymerase chain reaction for the detection of miRNA-29. And the results of diabetic retinopathy and diabetic patients without complication were compared to the expression of miRNA-29 was significantly increased, the difference was statistically significant (P0.01), diabetic group and healthy control group without complications, no obvious change in the expression of miRNA-29, the difference was not statistically significant (P0.05); group of patients with diabetic retinopathy and without complication in patients with diabetic patients, compared to healthy control group, The expression level of RAX protein decreased significantly, the difference was statistically significant (P0.05) conclusions over expression of miRNA-29 in serum of patients with diabetic retinopathy, the potential target gene of miRNA-29 PKR binding protein X (RAX) expression, which may be involved in pathogenesis of diabetic retinopathy, and cause diabetic retinopathy.

【作者单位】： 北华大学附属医院内分泌科;
【分类号】：R587.2;R774.1
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本文编号：1695519