糖尿病合并白内障患者血清及房水中铬元素含量测定及分析

发布时间：2018-04-09 06:19

本文选题：铬　切入点：房水　出处：《辽宁医学院》2012年硕士论文

【摘要】：目的本研究就国内外关于铬元素与糖尿病患者老年性白内障的发病及病情进展方面的关系进行含量测定比较及相关性的分析，以期对糖尿病患者老年性白内障的发病机制及病情的控制能有更进一步的了解。方法收集80例经临床确诊的患有老年性白内障，核成熟度为3-4级的患者：其中合并2型糖尿病患者有40例（实验组），非糖尿病患者40例（对照组），术前抽取空腹血并离心出血清保存，白内障超声乳化术前抽取前房水保存，采用石墨炉原子吸收法测定两组患者血清及房水中铬元素的含量进行比较及统计学分析，并对两组血清及房水中铬元素含量的相关性进行分析。结果所有实验标本经过严格的实验操作后均获得较为满意的实验结果。 1、实验组血清及房水中铬含量与年龄比较均无显著相关性（r=0.009，，p0.05，r=-0.038，p0.05），对照组血清及房水中铬含量与年龄间也无显著相关性（r=-0.045，p0.05，r=0.192，p0.05），实验组糖尿病病程与血清及房水中铬元素含量均无显著相关性，（r=0.378，p0.05，r=-0.067，p0.05）。 2、两组相同年龄段间分别比较，75岁以下实验组血清及房水中铬含量比对照组低，（t=-4.914，p0.05，t=-4.145，p0.05），75岁以上实验组血清及房水中铬含量比对照组低，（t=-4.233，p0.05，t=-4.679，p0.05）。 3、实验组房水中铬元素含量为（1.11±0.46）μg/L，显著低于对照组（2.10±0.89）μg/L，且两组间比较差异有统计学意义（t=-6.206，p0.05）。 4、实验组血清中铬元素含量（3.35±0.92）μg/L，也显著低于对照组（4.59±0.82）μg/L，两组间比较差异有统计学意义（t=-6.378，p0.05）。 5、实验组的血清及房水中铬元素含量进行相关性分析，结果示两者间有显著相关性（r=0.777，p0.05)，对照组血清及房水中铬元素含量进行相关性分析也具有显著相关性（r=0.608，p0.05）。结论 1、糖尿病合并白内障患者的血清及房水中铬元素的含量较非糖尿病老年性白内障组血清及房水中铬元素含量偏低。 2、铬对糖尿病患者血糖及前房中葡萄糖水平都有一定程度的调节作用，推测其对糖尿病患者白内障进展可能有一定控制作用 3、血清中铬元素含量与房水中铬元素含量比较具有相关性，故推测通过测定血清中铬可以在一定程度上评估房水铬含量。
[Abstract]:PurposeIn this study, the relationship between chromium and the incidence and progression of senile cataract in diabetic patients was analyzed.The aim is to further understand the pathogenesis and control of senile cataract in diabetic patients.MethodEighty cases of senile cataract diagnosed by clinical diagnosis were collected.There were 40 patients with type 2 diabetes mellitus (experimental group) and 40 patients with non-diabetes mellitus (control group). Fasting blood was taken and serum was centrifuged before cataract phacoemulsification, and aqueous humor was extracted before phacoemulsification.The content of chromium in serum and aqueous humor of the two groups was determined by graphite furnace atomic absorption spectrometry. The correlation of chromium content in serum and aqueous humor between the two groups was analyzed.ResultAll the specimens obtained satisfactory experimental results after strict operation.1. There was no significant correlation between chromium content in serum and aqueous humor of experimental group and age. There was no significant correlation between chromium content in serum and aqueous humor of control group and age. There was no significant correlation between chromium content in serum and aqueous humor of control group and age. The course of diabetes in experimental group and the content of chromium in serum and aqueous humor in experimental group were not significantly correlated with the content of chromium in serum and aqueous humor. The course of diabetes in experimental group was related to the content of chromium in serum and aqueous humor.There was no significant correlation between the two groups.2. The chromium content in serum and aqueous humor of the experimental group under 75 years old was lower than that of the control group. The content of chromium in the serum and aqueous humor of the experimental group over 75 years old was lower than that of the control group, and the content of chromium in the serum and aqueous humor of the experimental group was lower than that of the control group.3. The content of chromium in aqueous humor in the experimental group was 1.11 卤0.46 渭 g / L, which was significantly lower than that in the control group (2.10 卤0.89) 渭 g / L, and the difference between the two groups was statistically significant.4. The content of Cr in the serum of the experimental group was 3.35 卤0.92 渭 g / L, which was significantly lower than that of the control group (4.59 卤0.82) 渭 g / L, and the difference between the two groups was statistically significant.5. The content of chromium in serum and aqueous humor of the experimental group was analyzed. The results showed that there was a significant correlation between the content of Cr in serum and aqueous humor, and the content of Cr in serum and aqueous humor of the control group was also significantly correlated with the content of Cr in the serum and aqueous humor of the control group.Conclusion1. The content of Cr in serum and aqueous humor of diabetic patients with cataract was lower than that in non-diabetic senile cataract group.2. Chromium can regulate blood glucose and glucose level in anterior chamber of diabetic patients to a certain extent. It is speculated that chromium can control the development of cataract in diabetic patients to some extent.3. The content of chromium in serum is correlated with the content of chromium in aqueous humor, so it is speculated that the content of chromium in aqueous humor can be evaluated to a certain extent by determining the content of chromium in serum.
【学位授予单位】：辽宁医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R776.1

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