PHF8在喉癌下咽癌中的表达及临床意义

发布时间：2018-04-12 21:13

本文选题：喉癌和下咽癌 + PHF8　；参考：《中南大学》2012年硕士论文

【摘要】：目的：探讨组蛋白去甲基化酶PHD锌指蛋白8(PHF8)蛋白质在喉癌,下咽癌组织及癌旁组织中的表达,同时探讨其表达与喉癌,下咽癌临床病理特征和预后之间的关系。方法：采用免疫组织化学方法检测PHF8蛋白在95例喉癌,下咽癌中的表达情况,并统计分析PHF8蛋白与喉癌,下咽癌的临床病理特征及预后之间的关系；我们运用Western blot方法检测12例配对的喉癌,下咽癌及癌旁组织中的PHF8蛋白表达水平的差异。结果：PHF8蛋白在喉癌,下咽癌组织中表达上调。PHF8蛋白的表达与喉癌,下咽癌的肿瘤发生的位置(P=0.001)、T分级(P=0.008)、临床分期(P=0.039)、复发(P=0.023)及预后密切相关；Kaplan-Meier生存分析结果显示PHF8高表达组与低表达组比较,高表达组无瘤生存率低于低表达组且高表达组的整体生存率低于低表达组,多因素Cox比例风险回归模型分析进一步显示,PHF8蛋白表达水平可作为喉癌,下咽癌预后的独立影响因素(P=0.037)。结论：PHF8在喉癌,下咽癌组织中的表达上调,PHF8在喉癌,下咽癌患者高表达与肿瘤的位置、T分级、复发、临床分期、及患者的预后相关。结果显示PHF8在喉癌,下咽癌中的发生、发展中可能发挥了重要的作用,PHF8可能成为预测喉癌,下咽癌术后复发及预后的重要标志。
[Abstract]:Objective : To investigate the expression of the protein 8 ( PHF8 ) protein in laryngeal carcinoma , hypopharyngeal carcinoma and adjacent tissues , and to explore the relationship between its expression and the clinicopathological features and prognosis of laryngeal carcinoma and hypopharyngeal carcinoma .

Methods : The expression of PHF8 protein in 95 patients with laryngeal carcinoma and hypopharyngeal carcinoma was detected by immunohistochemistry . The relationship between PHF8 protein and the clinicopathological features and prognosis of laryngeal carcinoma and hypopharyngeal carcinoma was analyzed .
Western blot was used to detect the expression of PHF8 protein in 12 paired laryngeal carcinoma , hypopharyngeal carcinoma and paracancer tissues .

Results : The expression of PHF8 protein was up - regulated in laryngeal carcinoma and hypopharyngeal carcinoma . The expression of PHF8 protein was closely related to the location of laryngeal carcinoma and hypopharyngeal carcinoma ( P = 0.001 ) , T grade ( P = 0.008 ) , clinical stage ( P = 0.039 ) , recurrence ( P = 0.023 ) and prognosis .
The Kaplan - Meier survival analysis showed that the high expression level of PHF8 was lower than that in low expression group , and the overall survival rate of high expression group was lower than that in low expression group . Multivariate Cox proportional hazard regression model analysis showed that PHF8 protein expression level could be used as an independent prognostic factor for laryngeal cancer and hypopharyngeal carcinoma ( P = 0.037 ) .

Conclusion : The expression of PHF8 is up - regulated in laryngeal carcinoma and hypopharyngeal carcinoma . The expression of PHF8 is related to the location , T grade , recurrence , clinical stage and prognosis of patients with laryngeal carcinoma and hypopharyngeal carcinoma . The results show that PHF8 may play an important role in the development of laryngeal carcinoma and hypopharyngeal carcinoma .

【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.6

【参考文献】