角膜新生血管对角膜损伤神经再生影响的研究

发布时间：2018-04-27 09:42

本文选题：角膜神经 + 神经再生　；参考：《眼科》2017年02期

【摘要】：目的探讨角膜新生血管对大鼠角膜损伤神经再生的影响。设计实验研究。研究对象SD大鼠。方法采用随机数字表法将18只SD大鼠分为3组,每组6只。A组行缝线铲针角膜基质层间切开及缝线诱导新生血管术,术后0、3、7天给予结膜下注射贝伐单抗;B组行缝线铲针角膜基质层间切开及缝线诱导新生血管术;C组0、3、7天行结膜下注射贝伐单抗操作。分别在术后1天、1周、2周、4周,采用裂隙灯照相法观察记录角膜新生血管面积;角膜共聚焦显微镜记录神经长度。采用CochetBonnet知觉仪测量缝线区的角膜知觉,采用Schirmer试验泪液线测量右眼的泪液分泌量。术后4周角膜全层铺片免疫荧光染色,记录上皮下神经密度。主要指标角膜新生血管面积比、神经长度、上皮下神经密度、角膜知觉、泪液分泌量。结果 A、B组术后1、2周有角膜新生血管生长,术后4周消退闭锁,C组无角膜新生血管生长。A组术后1、2周新生血管面积比为(10.86±1.57)%和(1.87±0.69)%,分别小于B组的(25.42±2.65)%和(6.48±1.10)%(P均=0.000)。术后1天A、B组神经长度分别为(151.02±4.74)μm、(149.69±4.32)μm(P=0.306);术后1、2、4周,A组神经长度均长于B组,分别为(193.84±2.25)μm与(155.73±2.98)μm、(217.15±2.08)μm与(166.21±2.41)μm、(220.70±1.41)μm与(203.76±1.74)μm(P均=0.000)。术后A、B组神经长度均有减少并有恢复趋势,C组无明显变化。术后4周A组损伤区上皮下神经密度(22.60%±2.02%)明显高于B组(9.41%±2.01%)(P=0.000)。A、B组上皮下神经短小稀疏、密度低,C组形态正常。A、B组术后1、2、4周时角膜知觉及泪液分泌量均无统计学差异(P均0.05)。A、B组均有下降并恢复趋势,C组无明显变化。结论角膜新生血管可能抑制角膜损伤神经再生,抑制角膜新生血管有利于神经再生。
[Abstract]:Objective to investigate the effect of corneal neovascularization on nerve regeneration after corneal injury in rats. Design experimental research. Study object SD rats. Methods 18 SD rats were randomly divided into 3 groups. Each group (n = 6) received suture shovel interlaminar keratectomy and suture induced neovascularization. Group B was given subconjunctival injection of bevacizumab on 7 days after operation, and group C received subconjunctival injection of bevacizumab on 7 days after subconjunctival injection of the subconjunctival injection of bevacizumab. The corneal neovascularization area was observed by slit lamp photography and the nerve length was recorded by confocal microscope. Corneal perception in suture area was measured by CochetBonnet perceptron and tear discharge of right eye was measured by Schirmer test. Four weeks after operation, the whole corneal layer was stained with immunofluorescence and the nerve density was recorded. Main outcome measures: area ratio of corneal neovascularization, length of nerve, density of epigastric nerve, corneal perception and tear secretion. Results Corneal neovascularization was observed in group A at 1 and 2 weeks postoperatively, and the ratio of neovascularization area to neovascularization in group A was 10.86 卤1.57% and 1.87 卤0.69% respectively, which was less than that in group B (25.42 卤2.65%) and group C (6.48 卤1.10) (P = 0.000). The nerve length of group A was 151.02 卤4.74 渭 m and that of group A was 193.84 卤2.25 渭 m and 155.73 卤2.98 渭 m, respectively, and that of group A was 220.70 卤1.41 渭 m and 203.76 卤1.74 渭 m respectively, and that of group A was 220.70 卤1.41 渭 m and 203.76 卤1.74 渭 m, respectively, and the length of nerve in group A was 217.15 卤2.08 渭 m and 220.70 卤1.41 渭 m and 203.76 卤1.74 渭 m, respectively. After operation, the nerve length of group A B decreased and there was no obvious change of nerve length in group C. The density of subepithelial nerve in group A (22.60% 卤2.02) was significantly higher than that in group B (9.41% 卤2.01%) at 4 weeks after operation. There was no significant difference in corneal perception and tear secretion between group C and group C at 1 and 2 weeks after operation. There was no significant difference in corneal perception and tear secretion in group C (P < 0.05). There was no significant change in group C (P = 0.05), but there was no significant change in group C (P > 0.05). Conclusion Corneal neovascularization may inhibit nerve regeneration after corneal injury, and inhibition of corneal neovascularization is beneficial to nerve regeneration.
【作者单位】：首都医科大学附属北京同仁医院北京同仁眼科中心北京市眼科研究所眼科学与视觉科学北京重点实验室;
【分类号】：R772.2

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