姜黄素壳聚糖脂质体治疗兔角膜碱烧伤

发布时间：2018-05-28 22:33

本文选题：姜黄素 + 角膜新生血管　；参考：《国际药学研究杂志》2016年04期

【摘要】：目的研究姜黄素壳聚糖脂质体对兔角膜碱烧伤的治疗作用。方法采用薄膜分散法制备姜黄素壳聚糖脂质体,测定粒径、Zeta电位和包封率。新西兰大耳白兔随机分为生理盐水组、空白壳聚糖包衣脂质体组、地塞米松组和姜黄素壳聚糖脂质体组。建立碱烧伤模型并给予相应药物。裂隙灯观察角膜新生血管(CNV)生长情况及角膜上皮愈合情况,免疫组化法检测角膜血管内皮生长因子(VEGF)表达。结果姜黄素壳聚糖脂质体粒径为(96.6±14.7)nm,Zeta电位为(58.8±2.3)m V,包封率为(51.41±1.1)%。姜黄素壳聚糖脂质体组和地塞米松组均能有效抑制CNV生长,降低角膜VEGF表达。与地塞米松组比,姜黄素壳聚糖脂质体组能促进角膜上皮恢复。结论姜黄素壳聚糖脂质体包封率较高,抑制CNV生长,促进角膜上皮修复。姜黄素壳聚糖脂质体有望成为新型治疗角膜碱烧伤的眼用制剂。
[Abstract]:Objective to study the therapeutic effect of curcumin chitosan liposome on alkali burn of cornea in rabbits. Methods curcumin chitosan liposomes were prepared by thin-film dispersion method. The Zeta potential and encapsulation efficiency of curcumin chitosan liposomes were measured. New Zealand white rabbits were randomly divided into normal saline group, blank chitosan coated liposome group, dexamethasone group and curcumin chitosan liposome group. The model of alkali burn was established and the corresponding drugs were given. The growth of corneal neovascularization (CNV) and corneal epithelial healing were observed by slit lamp. The expression of corneal endothelial growth factor (VEGF) was detected by immunohistochemistry. Results the particle size of curcumin chitosan liposomes was 96.6 卤14.7 nmGV, the Zeta potential was 58.8 卤2.3 mV, the entrapment efficiency was 51.41 卤1.1 mV. Curcumin chitosan liposome group and dexamethasone group could effectively inhibit CNV growth and reduce corneal VEGF expression. Compared with dexamethasone group, curcumin chitosan liposome group could promote corneal epithelial recovery. Conclusion the encapsulation efficiency of curcumin chitosan liposome is high, which inhibits the growth of CNV and promotes corneal epithelial repair. Curcumin chitosan liposome is expected to be a new eye preparation for alkali burn of cornea.
【作者单位】：安徽医科大学研究生学院;武警总医院眼科;军事医学科学院放射与辐射医学研究所;
【基金】：国家发改委卫星示范专项资助项目〔(2013)2140号〕
【分类号】：R779.1

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