姜黄素对人鼻咽癌CNE-2细胞增殖及凋亡的影响

发布时间：2018-06-10 21:21

本文选题：姜黄素 + 鼻咽肿瘤　；参考：《重庆医学》2017年35期

【摘要】：目的探讨姜黄素对人鼻咽癌(NPC)CNE-2细胞增殖及凋亡的影响。方法不同浓度(0、10、20、40、60μmol/L)姜黄素处理NPC细胞株CNE-2,利用噻唑蓝(MTT)实验检测CNE-2细胞增殖活性,利用流式细胞仪检测CNE-2细胞周期及凋亡率,利用Hoechest33258荧光染色法观察细胞凋亡情况。结果姜黄素可明显抑制CNE-2细胞增殖作用,且随姜黄素浓度增加,CNE-2细胞抑制率呈上升趋势(P0.05),姜黄素作用CNE-2细胞24、48、72h的半抑制浓度(IC50)分别为(23.54±0.36)、(18.31±0.42)、(8.56±0.37)μmol/L,即姜黄素可明显抑制CNE-2细胞增殖作用,且呈现明显浓度、时间依赖性;流式细胞检测结果显示,0、10、20、40、60μmol/L姜黄素处理CNE-2细胞,凋亡率随着姜黄素浓度增加而上升;荧光染色结果可见,CNE-2细胞未给予姜黄素处理,细胞呈圆形或者椭圆形,细胞核大小均匀一致,染色质分布均匀的淡蓝色荧光;10μmol/L姜黄素处理CNE-2细胞24h后,细胞胞体缩小,细胞核染色质凝聚,呈颗粒状亮蓝色荧光;20μmol/L姜黄素处理CNE-2细胞24h后,细胞出现胞体缩小,细胞核浓缩、染色质不均匀,出现凋亡小体,甚至出现核碎裂;40和60μmol/L姜黄素处理CNE-2细胞24h后,细胞凋亡小体数量增多,出现大量核碎裂。结论姜黄素对NPC细胞株CNE-2细胞增殖具有明显抑制作用,且促进CNE-2细胞凋亡。
[Abstract]:Objective to investigate the effect of curcumin on proliferation and apoptosis of human nasopharyngeal carcinoma (NPC) NPC-CNE-2 cells. Methods the NPC cell line CNE-2 was treated with curcumin at different concentrations. The proliferative activity of CNE-2 cells was detected by thiazolyl methoate (MTT) assay, the cell cycle and apoptosis rate were detected by flow cytometry, and the apoptosis was observed by Hoechest33258 fluorescence staining. Results curcumin could significantly inhibit the proliferation of CNE-2 cells, and the inhibitory rate of curcumin on CNE-2 cells showed an increasing trend with the increase of curcumin concentration. The semi-inhibitory concentration of curcumin on CNE-2 cells was 23.54 卤0.36, 8.31 卤0.428.56 卤0.37 渭 mol / L, respectively. Curcumin could significantly inhibit the proliferation of CNE-2 cells. The results of flow cytometry showed that the apoptosis rate of CNE-2 cells increased with the increase of curcumin concentration, and the apoptosis rate of CNE-2 cells increased with the increase of curcumin concentration, and the results of fluorescence staining showed that the CNE-2 cells were not treated with curcumin, and the results of flow cytometry showed that the apoptosis rate of CNE-2 cells increased with the increase of curcumin concentration. The cells were round or oval, the nucleus size was uniform and the chromatin was evenly distributed. After treated with curcumin 10 渭 mol / L for 24 hours, the cell body was reduced and the nuclear chromatin condensed. After treated with curcumin 20 渭 mol 路L ~ (-1) in granular form for 24 hours, CNE-2 cells became smaller, nucleus concentrated, chromatin inhomogeneous, apoptotic bodies appeared, and even nuclear fragmentation 40 渭 mol / L and 60 渭 mol / L curcumin for 24 h after treatment of CNE-2 cells. The number of apoptotic bodies increased and a large number of nuclear fragmentation appeared. Conclusion curcumin can inhibit the proliferation of CNE-2 cells and promote the apoptosis of CNE-2 cells.
【作者单位】：苏州大学附属第二医院耳鼻喉科;镇江第一人民医院耳鼻喉科;
【基金】：2011年江苏省六大人才高峰项目(2011-021) 江苏省苏州市科技局资助项目(SYS201233)
【分类号】：R739.63

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