口咽鳞癌中HPV感染与血管生成的关系

发布时间：2018-06-28 18:34

本文选题：口咽癌 + 鳞状细胞癌　；参考：《现代肿瘤医学》2016年04期

【摘要】：目的:研究口咽鳞癌(oropharyngeal squamous cell carcinomas,OSCC)中人乳头瘤病毒(human papillomavirus,HPV)感染与血管内皮生长因子(vascular endothelial growth factor,VEGF)、CD31标记的微血管密度(microvessel density,MVD)表达的关系,探讨它们在口咽鳞癌发生中的相互关系。方法:采用多重实时荧光定量聚合酶链反应(multiplex real-time polymerase chain reaction,MT-PCR)检测60例口咽鳞癌HPV DNA及型别,通过HPV DNA分析进行HPV感染状况的测定;同时采用半定量免疫组化检测HPV(+)组和HPV(-)组中VEGF、CD31(MVD标志)蛋白表达情况。结果:口咽鳞癌中HPV感染率36.7%(22/60),HPV-16型占所有感染者的比例86.4%(19/22),明显高于其他型别;VEGF高度表达者出现MVD15个/高倍视野的几率较VEGF低度表达者高(P=0.001);MVD15个/高倍视野的肿瘤为低分化的可能性较高(P=0.004);VEGF和MVD表达与患者年龄、性别、TNM分期、HPV感染状况之间无相关性。结论:HPV感染与口咽鳞癌的发生存在相关性。口咽鳞癌中HPV感染状况和VEGF之间无相关性。
[Abstract]:Objective: to investigate the relationship between human papillomavirus infection and the expression of CD31 labeled microvessel density in oropharyngeal squamous cell carcinoma (OSCC), and to explore the relationship between human papillomavirus infection and CD31 labeled microvessel density in oropharyngeal squamous cell carcinoma (OSCC). Methods: multiplex real-time fluorescent quantitative polymerase chain reaction (MT-PCR) was used to detect HPVDNA and its types in 60 patients with oropharyngeal squamous cell carcinoma (OSCC). The expression of VEGF CD31 (MVD marker) in HPV (-) and HPV (-) groups was detected by semi-quantitative immunohistochemistry. Results: in oropharyngeal squamous cell carcinoma, HPV infection rate was 36.7% (22 / 60) and HPV-16 accounted for 86.4% (19 / 22) of all infected patients, which was significantly higher than that of other types of high expression VEGF with 15 MVD15 / high power visual fields than those with low VEGF expression (P0. 001). The expression of VEGF and MVD was more likely to be differentiated (P0. 004) and the expression of VEGF and MVD was associated with the age of the patients. There was no correlation between sex TNM staging and HPV infection. Conclusion there is a correlation between the infection of human papillomavirus (HPV) and the occurrence of oropharyngeal squamous cell carcinoma. There was no correlation between HPV infection and VEGF in oropharyngeal squamous cell carcinoma.
【作者单位】：昆明医科大学第三附属医院云南省肿瘤医院头颈外科;昆明医科大学;昆明医科大学第三附属医院云南省肿瘤医院病理科;
【基金】：留学人员科技活动项目择优资助基金资助项目(云南省2010年度启动类)
【分类号】：R739.63

【共引文献】