CTLA-4在慢性扁桃体炎发病机制中的作用

发布时间：2018-07-31 15:10

【摘要】：目的:探讨CTLA-4在慢性扁桃体炎发病中的作用,从炎症免疫反应软调节的角度为慢性扁桃体炎的药物治疗提供新的可能和理论依据。方法:从接受扁桃体全切术的患者中收取21例慢性扁桃体炎患者的扁桃体标本作为实验组,以15例扁桃体生理性肥大患者的扁桃体标本作为对照组。用苏木精-伊红染色法观察2组扁桃体组织的病理改变,用免疫荧光技术检测CTLA-4在2组中的表达差异和分布特征,用荧光定量PCR实验检测CTLA-4mRNA在2组中的相对表达定量。结果:苏木精-伊红染色显示实验组扁桃体病理结构以滤泡增生和生发中心的变化为主要特征,对照组发现有明显的"星空"现象;免疫荧光结果显示,实验组扁桃体组织中CTLA-4的平均荧光强度和密度明显高于对照组(P0.05);荧光定量PCR实验结果显示,实验组CTLA-4mRNA的相对表达量显著高于对照组(t=6.294,P0.01)。结论:CTLA-4在慢性扁桃体炎的发展中起重要作用,说明慢性扁桃体炎症的转归过程中可能存在免疫抑制的机制。扁桃体淋巴组织存在免疫抑制,为临床上扁桃体反复发炎的现象提供了新的解释,同时为慢性扁桃体炎的临床治疗提供了除手术以外从免疫学角度研发新的治疗药物的可能性。
[Abstract]:Objective: to investigate the role of CTLA-4 in the pathogenesis of chronic tonsillitis and to provide a new theoretical basis for the pharmacotherapy of chronic tonsillitis from the perspective of soft regulation of inflammatory immune response. Methods: the tonsils of 21 patients with chronic tonsillitis and 15 patients with physiologic hypertrophy of tonsil were collected from the patients undergoing total tonsillectomy as experimental group and control group. The pathological changes of tonsil in two groups were observed by hematoxylin and eosin staining. The difference and distribution of CTLA-4 expression in the two groups were detected by immunofluorescence technique, and the relative expression of CTLA-4mRNA in the two groups was detected by fluorescence quantitative PCR test. Results: hematoxylin-eosin staining showed that the pathological structure of the tonsils in the experimental group was characterized by follicular proliferation and germinal center. The average fluorescence intensity and density of CTLA-4 in tonsils of the experimental group were significantly higher than those in the control group (P0.05). The results of fluorescence quantitative PCR experiment showed that the relative expression of CTLA-4mRNA in the experimental group was significantly higher than that in the control group (tr 6.294, P0.01). Conclusion it is suggested that the mechanism of immunosuppression may exist in the progression of chronic tonsillitis, which may play an important role in the development of chronic tonsillitis. The existence of immunosuppression in tonsillar lymphoid tissue provides a new explanation for the phenomenon of recurrent tonsillitis in clinic and the possibility of developing new treatment drugs from immunological perspective besides surgery for the clinical treatment of chronic tonsillitis.
【作者单位】：安徽医科大学免疫学教研室及过敏与免疫研究中心;安徽医科大学第一附属医院耳鼻咽喉头颈外科;安徽医科大学第一附属医院检验科;
【基金】：国家自然科学基金(No:81273245) 2014年高等教育振兴计划人才项目基金
【分类号】：R766.18

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