自噬对鼻咽癌细胞CNE2放疗敏感性的调节作用

发布时间：2018-08-07 21:22

【摘要】：目的探讨自噬激活剂和自噬抑制剂分别对鼻咽癌细胞CNE2放疗敏感性的影响。方法利用RNA干扰技术使atg5基因沉默,构建自噬抑制细胞模型后,与雷帕霉素、氯喹分别处理的两组细胞一起,每天以X射线5Gy照射细胞,连续8天观察各组细胞的生长状况,并设置对照组。以MTT法及克隆集落形成法检测其细胞活力,用流式细胞仪分析其细胞周期。结果与对照组相比,其他三组细胞存活率、克隆形成率、照射后存活率均显著降低(P0.05);细胞周期检测除对照组外其他三组细胞集中在G0/G1期,其他两个时期比G0/G1期相对较少。结论自噬抑制剂与激活剂和atg5沉默均能为CNE2放疗增敏,然而自噬激活剂的增敏效果好于其他,为增敏放疗提供实验依据,开辟新的放疗增敏途径。
[Abstract]:Objective to investigate the effects of autophagy activator and autophagy inhibitor on the radiosensitivity of nasopharyngeal carcinoma (NPC) cell line CNE2. Methods the atg5 gene was silenced by RNA interference technique, and the autophagy inhibitory cell model was constructed. The cells were irradiated with X ray 5Gy for 8 days together with two groups of cells treated with rapamycin and chloroquine respectively, and the growth status of each group was observed for 8 days. The control group was set up. The cell viability was detected by MTT assay and colony forming assay, and the cell cycle was analyzed by flow cytometry. Results compared with the control group, the survival rate, clone formation rate and survival rate of the other three groups were significantly lower than those of the control group (P0.05). The cell cycle of the other three groups, except the control group, was concentrated in the G0/G1 phase, and the other two periods were relatively less than the G0/G1 phase. Conclusion both autophagy inhibitor and activator and atg5 silencing can increase the sensitivity of CNE2, but the sensitizing effect of autophagy activator is better than that of others, which provides experimental basis for sensitizing radiotherapy and opens up a new way of radiosensitization.
【作者单位】：广州医科大学附属广州市第一人民医院肿瘤科;广东药科大学公共卫生学院;
【基金】：2012年广东省科技计划基金(01078650166731031) 2011年度广东省医学科研基金(A2011469) 2011年度广州市医药卫生科技一般引导项目(201102A213075)
【分类号】：R739.63

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