白细胞介素-10调控巨噬细胞以促进眼底新生血管形成

发布时间：2018-08-19 19:13

【摘要】：目的·研究白细胞介素-10(IL-10)对眼底新生血管(NV)的影响。方法·采用免疫荧光、RT-PCR、Western blotting等方法检测在氧诱导视网膜病变(OIR)小鼠模型及VEGF过表达(rho/VEGF)转基因小鼠模型中IL-10的表达。应用IL-10基因敲除鼠,研究IL-10对视网膜、视网膜下及脉络膜新生血管的影响。结果·在OIR及rho/VEGF转基因小鼠模型中,IL-10的免疫荧光染色强度增加,IL-10的m RNA表达量也升高。与正常对照组小鼠相比,IL-10基因敲除鼠(IL-10-/-)由OIR诱导的新生血管和激光诱导脉络膜新生血管(CNV)均显著减少。并且,在IL-10基因敲除鼠中,其低氧诱导因子-1α(HIF-1α)以及血管内皮生长因子(VEGF)和血管内皮生长因子受体1(VEGFR1)的表达均减少。在IL-10基因敲除的OIR小鼠模型视网膜中,巨噬细胞受调控后数量减少。结论·IL-10通过刺激HIF-1α及VEGF、VEGFR1的表达而促进眼新生血管的形成。IL-10在视网膜缺氧时通过调控巨噬细胞,促进眼新生血管的形成。
[Abstract]:Objective to study the effect of interleukin-10 (IL-10) on fundus neovascularization (NV). Methods the expression of IL-10 in oxygen-induced retinopathy (OIR) mice and VEGF overexpression (rho/VEGF) transgenic mice was detected by immunofluorescence RT PCR Western blotting. The effects of IL-10 gene knockout mice on retinal, subretinal and choroidal neovascularization were studied. Results in OIR and rho/VEGF transgenic mice, the immunofluorescence staining intensity of IL-10 increased the expression of m RNA of IL-10. Compared with normal control mice, IL-10 gene knockout mice (IL-10 / -) induced by OIR and laser-induced choroidal neovascularization (CNV) were significantly reduced. Moreover, the expression of hypoxia inducible factor 1 伪 (HIF-1 伪), vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor 1 (VEGFR1) in IL-10 knockout mice was decreased. In the retina of OIR mice model with IL-10 knockout, the number of macrophages decreased after being regulated. Conclusion IL-10 stimulates the formation of neovascularization by stimulating the expression of HIF-1 伪 and VEGF VEGFR1. IL-10 promotes the formation of neovascularization by regulating macrophages during retinal hypoxia.
【作者单位】：上海交通大学医学院附属瑞金医院眼科;
【基金】：国家自然科学基金(81570853)~~
【分类号】：R77

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