IL-9、IL-10在慢性鼻窦炎性疾病中的表达及意义
发布时间：2018-08-20 20:09
【摘要】：目的 Th9细胞是新近发现的新型的功能性辅助性T细胞亚群,主要表达IL-9和IL-10两种细胞因子。IL.9.IL-10与多种疾病的发生发展密切相关,特别在呼吸系统炎性疾病中发挥重要作用。本文旨在观察IL-9.IL-10在慢性鼻窦炎性疾病中的表达及其与局部嗜酸性粒细胞浸润的关系,进一步探讨其在慢性鼻窦炎性疾病中的可能作用机理。 方法 选取住院接受治疗的慢性鼻-鼻窦炎(chronic rhinosinusitis,CRS)患者30例和真菌性鼻-鼻窦炎(fungal rhino-sinusitis,FRS)患者13例,常规行FESS手术,术中收集病变鼻窦窦腔粘膜组织,并以18例鼻腔鼻窦非炎性疾病患者为对照组,收集其鼻腔非炎粘膜组织。采用H-E染色法观察组织形态并检测嗜酸性粒细胞数；免疫组织化学S-P二步法检查组织中IL-9、IL-10蛋白相对含量；半定量逆转录-聚合酶链式反应(reverse transcriptase polymerase chain reaction,RT-PCR)检测组织中IL-9.IL.10基因mRNA表达。所有资料使用SPSS13.0统计分析软件进行统计分析,采用方差分析及Pearson相关性分析方法,P0.05为差异有统计学意义。 结果 (1)慢性鼻-鼻窦炎组与真菌性鼻-鼻窦炎组患者病变鼻窦粘膜组织内均见大量嗜酸性粒细胞浸润,明显高于对照组的鼻腔非炎粘膜组织,P0.05：(2)IL-9、IL-10蛋白在慢性鼻-鼻窦炎与真菌性鼻-鼻窦炎患者病变鼻窦粘膜组织内均呈阳性表达,且表达量明显高于鼻腔非炎粘膜组织,相比差异有统计学意义P0.05；但在慢性鼻-鼻窦炎与真菌性鼻-鼻窦炎组之间差异无统计学意义,P0.05；(3)慢性鼻-鼻窦炎组与真菌性鼻-鼻窦炎组患者病变鼻窦粘膜内IL-9基因和IL-10基因mRNA均有表达,且其相对表达量均高于对照组,P0.05；但在慢性鼻-鼻窦炎组与真菌性鼻-鼻窦炎组之间差异无统计学意义,P0.05；(4)在慢性鼻-鼻窦炎患者病变鼻窦粘膜内IL-9与IL-10蛋白相对表达量与局部嗜酸性粒细胞浸润数均存在明显相关性P0.05。 结论 (1)慢性鼻-鼻窦炎与真菌性鼻-鼻窦炎患者病变鼻窦粘膜内可见大量嗜酸性粒细胞浸润,为其重要病理性特征之一；(2)IL-9与IL-10在慢性鼻-鼻窦炎与真菌性鼻-鼻窦炎患者病变鼻窦粘膜均有高表达,二者可能参与鼻窦炎性疾病的发生发展；(3)IL-9、IL-10与局部嗜酸性粒细胞浸润密切相关,其可能通过嗜酸性粒细胞影响鼻窦炎性疾病的发生发展。
[Abstract]:Objective Th9 cells are a new type of functional helper T cell subsets. The expression of IL-9 and IL-10 cytokines, IL.9.IL-10, is closely related to the occurrence and development of many diseases. Especially in the respiratory inflammatory diseases play an important role. The purpose of this study was to investigate the expression of IL-9.IL-10 in chronic sinusitis and its relationship with local eosinophil infiltration, and to explore its possible mechanism in chronic sinusitis. Methods 30 patients with chronic rhinosinusitis and 13 patients with fungal rhino-sinusitis were selected for FESS operation. The non-inflammatory mucosa of nasal cavity and sinuses were collected from 18 patients with non-inflammatory diseases of nasal cavity and paranasal sinuses as control group. Tissue morphology was observed by H-E staining and the number of eosinophils was detected by immunohistochemistry S-P two-step method was used to detect the relative content of IL-9 and IL-10 protein in tissues. Semi-quantitative reverse transcription-polymerase chain reaction (reverse transcriptase polymerase chain RT-PCR) was used to detect the expression of IL-9.IL.10 gene mRNA in tissues. All the data were statistically analyzed by SPSS13.0 statistical analysis software. The difference was statistically significant by means of ANOVA and Pearson correlation analysis (P0.05). Results (1) in both chronic rhinosinusitis group and fungal rhinosinusitis group, a large number of eosinophils infiltrated in the mucosa of the nasal sinuses. The expression of IL-9 IL-10 protein was significantly higher in patients with chronic rhinosinusitis and fungal rhinosinusitis, and the expression of IL-9 IL-10 protein was significantly higher than that in non-inflammatory nasal mucosa tissue, and the expression of IL-9 IL-10 protein was significantly higher in patients with chronic rhinosinusitis and fungal rhinosinusitis. But there was no significant difference between chronic rhinosinusitis and fungal rhinosinusitis. (3) the expression of IL-9 gene and IL-10 gene mRNA in nasal mucosa of patients with chronic rhinosinusitis and fungal rhinosinusitis were higher than that of control group (P 0.05). But there was no significant difference between chronic rhinosinusitis group and fungal rhinosinusitis group (P 0.05). (4) in patients with chronic rhinosinusitis, there was a significant correlation between the relative expression of IL-9 and IL-10 protein and the number of eosinophil infiltration (P0.05). Conclusion (1) in patients with chronic rhinosinusitis and fungal rhinosinusitis, a large number of eosinophils infiltration can be seen in the mucosa of the nasal sinuses, which is one of the important pathological features. (2) IL-9 and IL-10 were highly expressed in the nasal mucosa of patients with chronic rhinosinusitis and fungal rhinosinusitis, which may be involved in the occurrence and development of sinusitis, (3) IL-9 IL-10 was closely related to local eosinophils infiltration. It may affect the development of sinusitis disease through eosinophil.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R765.41
本文编号：2194847
[Abstract]:Objective Th9 cells are a new type of functional helper T cell subsets. The expression of IL-9 and IL-10 cytokines, IL.9.IL-10, is closely related to the occurrence and development of many diseases. Especially in the respiratory inflammatory diseases play an important role. The purpose of this study was to investigate the expression of IL-9.IL-10 in chronic sinusitis and its relationship with local eosinophil infiltration, and to explore its possible mechanism in chronic sinusitis. Methods 30 patients with chronic rhinosinusitis and 13 patients with fungal rhino-sinusitis were selected for FESS operation. The non-inflammatory mucosa of nasal cavity and sinuses were collected from 18 patients with non-inflammatory diseases of nasal cavity and paranasal sinuses as control group. Tissue morphology was observed by H-E staining and the number of eosinophils was detected by immunohistochemistry S-P two-step method was used to detect the relative content of IL-9 and IL-10 protein in tissues. Semi-quantitative reverse transcription-polymerase chain reaction (reverse transcriptase polymerase chain RT-PCR) was used to detect the expression of IL-9.IL.10 gene mRNA in tissues. All the data were statistically analyzed by SPSS13.0 statistical analysis software. The difference was statistically significant by means of ANOVA and Pearson correlation analysis (P0.05). Results (1) in both chronic rhinosinusitis group and fungal rhinosinusitis group, a large number of eosinophils infiltrated in the mucosa of the nasal sinuses. The expression of IL-9 IL-10 protein was significantly higher in patients with chronic rhinosinusitis and fungal rhinosinusitis, and the expression of IL-9 IL-10 protein was significantly higher than that in non-inflammatory nasal mucosa tissue, and the expression of IL-9 IL-10 protein was significantly higher in patients with chronic rhinosinusitis and fungal rhinosinusitis. But there was no significant difference between chronic rhinosinusitis and fungal rhinosinusitis. (3) the expression of IL-9 gene and IL-10 gene mRNA in nasal mucosa of patients with chronic rhinosinusitis and fungal rhinosinusitis were higher than that of control group (P 0.05). But there was no significant difference between chronic rhinosinusitis group and fungal rhinosinusitis group (P 0.05). (4) in patients with chronic rhinosinusitis, there was a significant correlation between the relative expression of IL-9 and IL-10 protein and the number of eosinophil infiltration (P0.05). Conclusion (1) in patients with chronic rhinosinusitis and fungal rhinosinusitis, a large number of eosinophils infiltration can be seen in the mucosa of the nasal sinuses, which is one of the important pathological features. (2) IL-9 and IL-10 were highly expressed in the nasal mucosa of patients with chronic rhinosinusitis and fungal rhinosinusitis, which may be involved in the occurrence and development of sinusitis, (3) IL-9 IL-10 was closely related to local eosinophils infiltration. It may affect the development of sinusitis disease through eosinophil.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R765.41
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