灼烧技术对慢性扁桃体炎患者TLR-NF-κB信号通路及相关细胞因子的影响研究

发布时间：2018-09-08 21:12

【摘要】：目的:通过对慢性扁桃体炎患者灼烧技术治疗前后TLR-NF-κB信号通路中的重要参与物质及部分相关细胞因子的浓度与健康志愿者相同指标的浓度进行比较研究,探讨该治疗方法对慢性扁桃体炎患者免疫功能所产生的影响和作用机制。方法:选取于我院2016年4月至2016年10月间门诊就诊并诊断为慢性扁桃体炎的患者20例为试验组,将其在灼烧技术治疗前以及一疗程灼烧技术治疗后采静脉血,将所得标本离心分离后获得血清,然后采用酶联免疫吸附试验检测TLR-NF-κB信号通路中TLR2、TLR4、NF-κB在外周血的浓度,同时检测细胞因子IL-4、IL-5、IL-10、IFN-γ及TGF-β在外周血的浓度,将患者治疗前后以上指标的浓度进行对比。同时招募健康志愿者6例作为对照组,静脉采血后分离得到血清,并采用相同方法检测以上指标的浓度,将试验组灼烧治疗前患者血清中以上指标的表达情况与对照组进行比较。所得数据采用SPSS21.0进行统计分析。结果:将试验组灼烧治疗前血清中所检测指标的浓度与对照组的进行比较,前者血清中TLR2、TLR4、NF-κB、IL-4、IL-5、IL-10、IFN-γ及TGF-β的浓度明显高于对照组,差异有统计学意义(P0.05);将试验组患者灼烧治疗前与治疗后进行比较,慢性扁桃体炎患者灼烧治疗后其血清中TLR2、TLR4、IL-4、IL-5、IL-10、NF-κB、IFN-γ及TGF-β的浓度比治疗前低,差异有统计学意义(P0.05);将试验组患者灼烧治疗前血清中TLR2、TLR4、IL-4、IL-5、IL-10、IFN-γ、TGF-β与NF-κB进行关联性分析,统计分析表明以上检测指标与NF-κB不存在关联性(P0.05)。而将慢性扁桃体炎患者灼烧治疗后血清中TLR2、TLR4、IL-4、IL-5、IL-10、IFN-γ、TGF-β与NF-κB进行关联性分析,统计分析表明灼烧治疗后血清中TLR2与NF-κB的呈正相关,而其他检测指标不存在关联性(P0.05)。结论:血清中TLR2、TLR4、IL-4、IL-5、IL-10、NF-κB、IFN-γ、TGF-β均参与了慢性扁桃体炎患者疾病的发生与发展,灼烧技术对该病的治疗作用与干预上述指标的表达关系密切,该治疗方式治疗慢性扁桃体炎的机制与干预TLR-NF-κB信号通路及该路径的部分细胞因子相关,且与TLR2所参与的TLR-NF-κB信号通路关系密切。
[Abstract]:Objective: to compare the concentrations of important involved substances and some related cytokines in TLR-NF- 魏 B signaling pathway between patients with chronic tonsillitis and healthy volunteers before and after burn therapy. To investigate the effect and mechanism of this treatment on immune function in patients with chronic tonsillitis. Methods: twenty patients with chronic tonsillitis from April 2016 to October 2016 in our hospital were selected as experimental group. Venous blood was collected before burning and after one course of burning. The serum was obtained by centrifugation. The concentration of TLR2,TLR4,NF- 魏 B in peripheral blood and the concentration of cytokines IL-4,IL-5,IL-10,IFN- 纬 and TGF- 尾 in peripheral blood of TLR-NF- 魏 B signaling pathway were detected by enzyme-linked immunosorbent assay (Elisa). The concentrations of the above indexes were compared before and after treatment. At the same time, 6 healthy volunteers were recruited as the control group, the serum was isolated after venous blood sampling, and the concentration of the above indexes was detected by the same method. The expression of the above indexes in serum of patients in the experimental group before burn treatment was compared with that in the control group. The data were analyzed by SPSS21.0. Results: the serum concentrations of TLR2,TLR4,NF- 魏 B, IL-4, IL-5, IL-10, IFN- 纬 and TGF- 尾 in the experimental group were significantly higher than those in the control group before burn treatment. The difference was statistically significant (P0.05). The serum levels of TLR2,TLR4,IL-4,IL-5,IL-10,NF- 魏 B, IFN- 纬 and TGF- 尾 in patients with chronic tonsillitis were lower than those before and after treatment. The correlation between TLR2,TLR4,IL-4,IL-5,IL-10,IFN- 纬 TGF- 尾 and NF- 魏 B was analyzed before burn treatment in the experimental group (P0.05). The statistical analysis showed that the above indexes were not correlated with NF- 魏 B (P0.05). The correlation between TLR2,TLR4,IL-4,IL-5,IL-10,IFN- 纬 TGF- 尾 and NF- 魏 B in serum of patients with chronic tonsillitis after burn treatment was analyzed. The statistical analysis showed that there was a positive correlation between TLR2 and NF- 魏 B in serum after burn treatment, but there was no correlation between TLR2 and NF- 魏 B in serum of patients with chronic tonsillitis (P0.05). Conclusion: the serum TLR2,TLR4,IL-4,IL-5,IL-10,NF- 魏 B of IFN- 纬 TGF- 尾 is involved in the occurrence and development of chronic tonsillitis. The therapeutic effect of burning is closely related to the expression of the above indexes. The mechanism of the treatment of chronic tonsillitis is related to the interference of TLR-NF- 魏 B signaling pathway and some cytokines of this pathway, and is closely related to the TLR-NF- 魏 B signaling pathway involved by TLR2.
【学位授予单位】：西南医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R766.18

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