雷公藤甲素在LPS诱导的急性葡萄膜炎中的抗炎作用

发布时间：2018-09-13 10:39

【摘要】：目的:观察雷公藤甲素对细菌脂多糖(LPS)诱导的急性葡萄膜炎(LIU)的抗炎作用。方法:BALB/c小鼠24只,随机分为空白对照组、模型组和雷公藤甲素组,每组8只,雷公藤甲素组给予0.05%雷公藤甲素滴眼液,空白对照组和模型组均给予等体积的生理盐水。连续给药30 d后,除空白对照组外各组小鼠于玻璃体腔内注射125 mg/L的LPS建立LIU模型。分别在造模后12、24、48和72 h后,对各组小鼠眼前节进行裂隙灯检查,并对眼组织进行病理学观察,同时提取眼组织蛋白,ELISA法检测眼组织中细胞间黏附分子1(ICAM-1)、白细胞介素1β(IL-1β)和单核细胞趋化蛋白1(MCP-1)的水平。结果:造模24 h后,与模型组相比,雷公藤甲素组能明显减轻LIU引起的炎症反应并降低炎症临床评分,差异有统计学意义(P0.01)。组织病理学也表明雷公藤甲素对小鼠前房、玻璃体腔内的炎症细胞浸润以及视网膜水肿增厚有明显的抑制作用。同时,ELISA结果显示雷公藤甲素显著下调眼组织中ICAM-1、IL-1β和MCP-1的表达,差异有统计学意义(P0.05)。结论:雷公藤甲素可能通过下调炎症细胞因子表达,从而减轻葡萄膜炎的眼部组织损伤,为葡萄膜炎的治疗提供了新的免疫疗法。
[Abstract]:Objective: To observe the anti-inflammatory effect of triptolide on acute uveitis (LIU) induced by bacterial lipopolysaccharide (LPS). Methods: Twenty-four BALB/c mice were randomly divided into blank control group, model group and triptolide group, with 8 mice in each group, triptolide group was given 0.05% triptolide eye drops, blank control group and model group were given the same volume of growth. After 30 days of continuous administration of normal saline, the mice in each group were injected with 125 mg/L L PS into the vitreous cavity to establish LIU model. After 12, 24, 48 and 72 hours of modeling, slit lamp examination was performed on the anterior segment of each group of mice, and the eye tissues were observed pathologically. At the same time, the protein of eye tissues was extracted and the cells in the eye tissues were detected by ELISA. Intercellular adhesion molecule-1 (ICAM-1), interleukin-1 beta (IL-1beta) and monocyte chemoattractant protein-1 (MCP-1) levels. Results: After 24 hours of modeling, compared with the model group, Tripterygium wilfordii could significantly reduce the inflammatory response induced by LIU and the clinical score of inflammation, the difference was statistically significant (P 0.01). Histopathology also showed that Tripterygium wilfordii was small. At the same time, ELISA results showed that triptolide significantly decreased the expression of ICAM-1, IL-1 beta and MCP-1 in the ocular tissues, and the difference was statistically significant (P 0.05). Conclusion: Tripterygium wilfordii may down-regulate the expression of inflammatory cytokines, thereby reducing the expression of ICAM-1, IL-1 beta and MCP-1. Ocular tissue injury of uveitis provides a new immunotherapy for the treatment of uveitis.
【作者单位】：中山大学中山眼科中心眼科学国家重点实验室;
【基金】：国家自然科学基金资助项目(No.81500739) 广东省中医药局科研项目(No.20151168) 广东省医学科学技术研究基金资助项目(No.A2015170)
【分类号】：R773.9

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