基于SERS技术的结肠癌与鼻咽癌免疫检测研究

发布时间：2018-09-19 12:35

【摘要】：表面增强拉曼散射(surface-enhanced Raman scattering, SERS)技术作为鉴定生物分子种类最有力的分析工具之一,具有灵敏度高、特异性强、稳定性好及检测条件温和等优点。目前,SERS技术应用于肿瘤病理领域处于起步阶段,但已显现出良好的应用前景和发展空间。随着检测设备、激光技术及纳米制备技术的进展,可以预见SERS技术将会成为临床病理和实验室诊断以及病理学研究中强有力的检测手段和分析工具,无论是在癌症的检测,还是在判断预后、监控疗效等方面都将发挥重要的作用。本文应用不同活性基底,制备相应的SERS免疫探针,对结肠癌以及鼻咽癌组织、结肠癌血液样品进行SERS免疫检测,主要的研究内容如下：首先,采用金核银壳纳米粒子为增强基底,以4-巯基苯甲酸(4-MBA)为SERS标记分子,制备CEA-SERS免疫探针,用于检测结肠癌组织切片中CEA的表达,并在结肠癌上皮细胞上检测到很好的探针的SERS信号；其次利用银纳米粒子为增强基底,制备结晶紫(CV)标记和4-巯基苯甲酸(4-MBA)标记的SERS免疫探针对结肠癌组织进行双组分免疫检测；然后又利用4-巯基苯甲酸(4-MBA)标记CEA-SERS免疫探针对结肠癌血液中CEA的值并进行定量检测；最后利用4-巯基苯甲酸(4-MBA)标记LPM1-SERS免疫探针对鼻咽癌上皮组织进行SERS免疫检测,并取得较好的结果。研究表明,利用SERS探针进行免疫检测的方法具有简单、快速、易行等优点；同时对生物样品进行双组分免疫检测,提高了分析效率,可以在较短的时间内同时得到多种待测物的分析结果,从而缩短了检测的时间,简化了分析过程,降低了检测成本,具有较高的应用价值。
[Abstract]:Surface enhanced Raman scattering (surface-enhanced Raman scattering, SERS) is one of the most powerful analytical tools for the identification of biomolecules. It has the advantages of high sensitivity, high specificity, good stability and mild detection conditions. The application of SERS technique in tumor pathology is still in its infancy, but it has a good application prospect and developing space. With the development of detection equipment, laser technology and nano-fabrication technology, it can be predicted that SERS technology will become a powerful tool for detection and analysis in clinical pathology and laboratory diagnosis and pathology research, whether in cancer detection. It will also play an important role in judging prognosis and monitoring curative effect. In this paper, using different active substrates, the corresponding SERS immunoprobe was prepared, and the samples of colon cancer, nasopharyngeal carcinoma and colon cancer blood were detected by SERS immunoassay. The main contents of this study were as follows: first, Gold core silver shell nanoparticles were used as enhancement substrate and 4-mercaptobenzoic acid (4-MBA) as SERS labeled molecule to prepare CEA-SERS immunoprobe to detect the expression of CEA in colon cancer tissue sections. The SERS signal of the probe was detected in colon cancer epithelial cells. Secondly, silver nanoparticles were used as the enhancement substrate. Preparation of crystal violet (CV) labeled and 4-mercaptobenzoic acid (4-MBA) labeled SERS immunosorbent probe for the detection of colon cancer tissue two-component immunoassay, and then using 4-mercaptobenzoic acid (4-MBA) labeled CEA-SERS probe to detect the value of CEA in the blood of colon cancer and quantitative detection. Finally, 4-mercaptobenzoic acid (4-MBA) labeled LPM1-SERS immunosorbent probe was used to detect SERS in nasopharyngeal carcinoma (NPC) epithelium, and good results were obtained. The results show that the immunoassay using SERS probe is simple, rapid and easy to carry out, and the two-component immunoassay for biological samples improves the efficiency of analysis. In a short time, the analytical results of a variety of objects to be tested can be obtained simultaneously, thus shortening the detection time, simplifying the analysis process and reducing the detection cost, so it has a higher application value.
【学位授予单位】：福建师范大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R735.35;R739.6

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