干眼患者眼表宏基因组研究

发布时间：2018-10-05 09:34

【摘要】：目的通过比较干眼患者和健康受试者眼表宏基因组的异同探讨眼表微生物组的改变在干眼发病机制中的作用。方法在解放军总医院眼科和中山大学中山眼科中心收集干眼患者20例及健康受试者90名。对所有受试者双眼进行结膜印迹细胞样本采集,提取核酸后进行宏基因组测序。通过计算Shannon指数比较两者微生物群落α多样性的差异;对两者微生物种群的相对丰度及代谢途径进行生物信息学分析。并对微生物中的抗生素抗性基因进行比对。结果干眼患者眼表微生物的α多样性与健康受试者相比差异无统计学意义(P=0.13)。干眼患者眼表15种微生物的相对丰度较高,健康受试者10种微生物的相对丰度较高,且在代谢途径上存在着差异。干眼患者眼表微生物中的抗生素抗性基因明显多于健康受试者。结论虽然干眼患者眼表微生物的多样性与健康受试者相似,但某些特定微生物种群的相对丰度及代谢途径存在特征性的改变,表明这些特定的微生物种群可能与干眼的发病机制有关。
[Abstract]:Objective to investigate the role of microorganism in the pathogenesis of dry eye by comparing the difference of macrogenome between dry eye patients and healthy subjects. Methods Twenty patients with dry eye and 90 healthy subjects were collected from ophthalmology center of PLA General Hospital and Zhongshan Ophthalmology Center of Sun Yat-sen University. Conjunctival imprinted cell samples were collected from the eyes of all subjects. The difference of 伪 -diversity of microbe community was compared by calculating Shannon index, and the relative abundance and metabolic pathway of microbe population were analyzed by bioinformatics. The antibiotic resistance genes in microorganisms were compared. Results there was no significant difference in 伪-diversity of microbes between dry eye patients and healthy subjects (P < 0. 13). The relative abundance of 15 microbes on the surface of dry eyes was higher than that of 10 microbes in healthy subjects, and there were differences in metabolic pathways. Antibiotic resistance genes in ocular surface microbes in dry eye patients were significantly higher than those in healthy subjects. Conclusion although the diversity of microbes in dry eye patients is similar to that in healthy subjects, the relative abundance and metabolic pathway of some specific microbial populations have characteristic changes. These specific microbial populations may be related to the pathogenesis of dry eyes.
【作者单位】：解放军总医院眼科;中山大学中山眼科中心眼科学国家重点实验室;
【基金】：国家自然科学基金面上项目(编号:31271400、31470065) 国家重点基础研究发展计划(编号:2013CB967001)~~
【分类号】：R777.34

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