共聚焦显微镜下干眼症患者角膜形态变化的诊断价值初探

发布时间：2018-10-07 20:03

【摘要】：目的应用共聚焦显微镜观察干眼症患者的角膜形态改变。方法运用日本Nidek公司共聚焦显微镜(Confoscan 4.0)对正常人群和干眼症患者各50例(100只眼)的中央区角膜上皮细胞的密度和异型性、上皮下神经纤维形态、朗格汉斯细胞的浸润和内皮细胞密度进行观察,并对结果进行统计学分析。结果共聚焦显微镜观察下,干眼症患者和正常人群的角膜中央区表层鳞状上皮细胞、翼状上皮细胞、上皮基底层细胞的细胞密度比较差异有统计学意义(P0.05)。干眼症患者的中央区角膜上皮细胞异型性增多(t=14.3,P0.01),少量细胞呈激活状态,上皮下神经纤维明显增粗,走行紊乱,伴朗格汉斯细胞浸润,内皮细胞计数和形态差异无统计学意义。结论共聚焦显微镜可以从细胞层面实时、无创观测干眼症患者的角膜形态的改变,根据角膜上皮细胞细胞密度和异型性、上皮下神经纤维形态、朗格汉斯浸润等病理变化直观识别和诊断干眼症。
[Abstract]:Objective to observe the corneal morphology in patients with xerophthalmia by confocal microscope. Methods Nidek confocal microscope (Confoscan 4.0) was used to study the density and heterogeneity of corneal epithelial cells in the central area of 50 normal subjects and 50 dry eye patients (100 eyes), and the morphology of epigastric nerve fibers. The infiltration of Langerhans cells and the density of endothelial cells were observed and the results were analyzed statistically. Results under confocal microscope, there were significant differences in cell density between dry eye patients and normal subjects in the surface squamous epithelium, airfoil epithelium and basal layer cells of cornea (P0.05). In dry eye patients, the corneal epithelial cells in the central area were increased (t: 14. 3 / P0.01), a small number of cells were activated, and the nerve fibers of the upper subcutaneous were thickened and disordered, accompanied by Langerhans' cell infiltration, and there was no significant difference in the number and morphology of endothelial cells. Conclusion Confocal microscope can be used to observe the changes of corneal morphology in patients with xerophthalmia at the cell level in real time. According to the density and heterogeneity of corneal epithelial cells, the morphology of epigastric nerve fibers can be observed. Langerhans infiltration and other pathological changes visual recognition and diagnosis of dry eye.
【作者单位】：安徽医科大学附属省立医院眼科;安徽省第二人民医院眼科;安徽医科大学组织胚胎学教研室;
【基金】：安徽省公益性技术应用研究联动计划项目(编号:1501ld04025)
【分类号】：R777.34

【相似文献】