饱和氢气生理盐水对大鼠视网膜蓝光损伤的保护作用
发布时间：2018-10-13 17:02
【摘要】：目的：视网膜光损伤,主要与活性氧(ROS)的堆积有关,是ARMD、RP及其他多种视网膜疾病重要机制。氢气是一种选择性的抗氧化剂,氢气溶解在液体中可选择性中和羟自由基和过氧亚硝酸阴离子,而后两者是氧化损伤最重要的介质。本实验中,我们探讨饱和氢气生理盐水溶液对大鼠视网膜蓝光损伤的作用。 方法：取100只健康雌性大鼠(160g-180g),随机分为四组。四组大鼠饲养于12H明12H暗环境中,并可自由摄取饮食。造模期间,一组不做处理,二组及三组按照大鼠体重分别予以1ml/100g生理盐水和饱和氢气生理盐水腹腔注射,四组作为对照组。二十天后,以蓝光照射6小时建立大鼠视网膜光损伤模型。于光照后8小时,16小时和24小时检测视网膜相关指标。样本取材后,通过H-E染色,超微结构观察,生化指标检测,观察视网膜损伤程度及饱和氢气生理盐水的作用。 结果：通过H-E检测发现,一组和二组视网膜结构损伤重,三组损伤轻。一组与二组间无明显差异。与一组和二组相比,三组的视网膜结构层次清晰,细胞排列整齐。400倍镜下可见,除了一组与二组之间,不同时间点各组之间的外核层厚度比较有明显差异(p0.05)。三个时间点,一组的外核层厚度为30.41±4.04μm,26.11±2.82μm和20.63±1.06μm,二组为31.62±4.5μm,25.08±3.63μm和19.07±3.86μm,三组为29.75±3.62μm,28.83±1.97μm和27.61±1.813μm,四组的平均厚度为37.35±1.37μmm。由此得出,随着时间延长,损伤不断加重。在24小时时,损伤的差别最大。与四组相比,一组薄46.23%,二组薄50.29%,三组薄28.04%。电镜下可见,三组损伤明显较一组和二组轻。在各时间点与四组相比,一组内二醛的含量明显增高(p0.05)。三组丙二醛含量明显低于一组(p0.05)和二组(p0.05)。在一组和二组之间比较,各时间点无明显差异(p0.05)。 结论：检测的结果显示,饱和氢气生理盐水能有效的保护视网膜,减轻光损伤,这种保护作用跟氢气的抗氧化作用有关。这些发现,使氢气成为治疗ARMD等光损伤导致的氧化应激相关视网膜病变的潜在药物。
[Abstract]:Objective: retinal light injury, mainly related to the accumulation of reactive oxygen species (ROS), is an important mechanism of ARMD,RP and many other retinal diseases. Hydrogen is a selective antioxidant. Hydrogen dissolved in liquid can selectively neutralize hydroxyl radical and peroxynitrite anion which are the most important medium for oxidative damage. In this experiment, we investigated the effects of saturated hydrogen saline solution on retinal blue light injury in rats. Methods: 100 healthy female rats (160g-180g) were randomly divided into four groups. The rats of the four groups were fed in 12 H light 12 H dark environment and could eat freely. During the modeling period, one group was not treated, two and three groups were given 1ml/100g saline and saturated hydrogen saline intraperitoneally according to the weight of the rats, and the four groups were taken as the control group. After 20 days, the rat model of retinal light injury was established after 6 hours of blue light irradiation. Retinal parameters were measured at 8 hours, 16 hours and 24 hours after illumination. After sampling, the degree of retinal injury and the effect of saturated hydrogen saline were observed by H-E staining, ultrastructure observation and biochemical index detection. Results: the results of H-E test showed that the damage of retinal structure was severe in one group and two groups, and light in three groups. There was no significant difference between one group and two groups. Compared with one group and two groups, the retinal structure in the three groups was clear and the cells were arranged neatly. The thickness of the outer nuclear layer was significantly different between each group except one group and two groups (p0.05). At three time points, the thickness of outer nuclear layer was 30.41 卤4.04 渭 m, 26.11 卤2.82 渭 m and 20.63 卤1.06 渭 m in one group, 31.62 卤4.5 渭 m, 25.08 卤3.63 渭 m and 19.07 卤3.86 渭 m in group two, 29.75 卤3.62 渭 m, 28.83 卤1.97 渭 m and 27.61 卤1.813 渭 m in group 3, and 37.35 卤1.37 渭 mm. in group 4, respectively. From this, it is concluded that as time goes on, the damage becomes more and more serious. At 24 hours, the difference was greatest. Compared with the four groups, one group was 46.23, the second group was 50.29, and the third group was 28.04 thin. Electron microscope showed that the injury in the three groups was significantly lighter than that in the first and second groups. Compared with the four groups, the content of dialdehyde in one group was significantly higher than that in the four groups at each time point (p 0.05). The malondialdehyde content in the three groups was significantly lower than that in the first group (p 0.05) and the second group (p 0.05). There was no significant difference between one group and two groups at each time point (p0.05). Conclusion: the results show that saturated hydrogen saline can effectively protect retina and reduce light damage, which is related to the antioxidant effect of hydrogen. These findings make hydrogen a potential drug in the treatment of oxidative stress-related retinopathy caused by light damage such as ARMD.
【学位授予单位】：华中科技大学
【学位级别】：博士
【学位授予年份】：2012
【分类号】：R774.1
本文编号：2269330
[Abstract]:Objective: retinal light injury, mainly related to the accumulation of reactive oxygen species (ROS), is an important mechanism of ARMD,RP and many other retinal diseases. Hydrogen is a selective antioxidant. Hydrogen dissolved in liquid can selectively neutralize hydroxyl radical and peroxynitrite anion which are the most important medium for oxidative damage. In this experiment, we investigated the effects of saturated hydrogen saline solution on retinal blue light injury in rats. Methods: 100 healthy female rats (160g-180g) were randomly divided into four groups. The rats of the four groups were fed in 12 H light 12 H dark environment and could eat freely. During the modeling period, one group was not treated, two and three groups were given 1ml/100g saline and saturated hydrogen saline intraperitoneally according to the weight of the rats, and the four groups were taken as the control group. After 20 days, the rat model of retinal light injury was established after 6 hours of blue light irradiation. Retinal parameters were measured at 8 hours, 16 hours and 24 hours after illumination. After sampling, the degree of retinal injury and the effect of saturated hydrogen saline were observed by H-E staining, ultrastructure observation and biochemical index detection. Results: the results of H-E test showed that the damage of retinal structure was severe in one group and two groups, and light in three groups. There was no significant difference between one group and two groups. Compared with one group and two groups, the retinal structure in the three groups was clear and the cells were arranged neatly. The thickness of the outer nuclear layer was significantly different between each group except one group and two groups (p0.05). At three time points, the thickness of outer nuclear layer was 30.41 卤4.04 渭 m, 26.11 卤2.82 渭 m and 20.63 卤1.06 渭 m in one group, 31.62 卤4.5 渭 m, 25.08 卤3.63 渭 m and 19.07 卤3.86 渭 m in group two, 29.75 卤3.62 渭 m, 28.83 卤1.97 渭 m and 27.61 卤1.813 渭 m in group 3, and 37.35 卤1.37 渭 mm. in group 4, respectively. From this, it is concluded that as time goes on, the damage becomes more and more serious. At 24 hours, the difference was greatest. Compared with the four groups, one group was 46.23, the second group was 50.29, and the third group was 28.04 thin. Electron microscope showed that the injury in the three groups was significantly lighter than that in the first and second groups. Compared with the four groups, the content of dialdehyde in one group was significantly higher than that in the four groups at each time point (p 0.05). The malondialdehyde content in the three groups was significantly lower than that in the first group (p 0.05) and the second group (p 0.05). There was no significant difference between one group and two groups at each time point (p0.05). Conclusion: the results show that saturated hydrogen saline can effectively protect retina and reduce light damage, which is related to the antioxidant effect of hydrogen. These findings make hydrogen a potential drug in the treatment of oxidative stress-related retinopathy caused by light damage such as ARMD.
【学位授予单位】：华中科技大学
【学位级别】：博士
【学位授予年份】：2012
【分类号】：R774.1
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