白皮杉醇对青光眼大鼠视网膜神经节细胞的保护作用

发布时间：2018-10-15 10:41

【摘要】：目的观察白皮杉醇对青光眼大鼠视网膜神经节细胞(retinal ganglion cells,RGCs)的保护作用并探讨其可能的机制。方法将40只大鼠随机分为对照组、模型组、白皮杉醇低剂量组和白皮杉醇高剂量组。采用光凝法建立青光眼大鼠模型,白皮杉醇低、高剂量组分别给予100 mg·kg~(-1)和200 mg·kg~(-1)白皮杉醇灌胃。测量各组大鼠眼压;荧光金逆行标记各组大鼠RGCs并计数;HE染色观察各组大鼠视网膜组织病理形态;免疫印迹法检测各组大鼠视网膜组织中磷酸化原癌基因蛋白Jun(proto oncogene protein Jun,c-Jun)氨基末端激酶(phosphorylation c-Jun N-terminal kinase,p-JNK)、磷酸化c-Jun(phosphorylation c-jun,p-c-Jun)、磷酸化细胞外调节蛋白激酶(phosphorylation extracellular regulated protein kinases,p-ERK)、磷酸化丝裂原活化蛋白激酶p38(phosphorylation mitogen activated protein kinases p38,p-p38 MAPK)和肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)蛋白表达。结果模型组眼压、p-JNK、p-c-Jun、p-ERK、p-p38 MAPK和TNF-α蛋白表达均显著高于对照组(均为P0.05),RGCs低于对照组(P0.05),视网膜组织结构可见空泡和水肿样改变。与模型组相比,白皮杉醇低剂量组和白皮杉醇高剂量组大鼠RGCs显著增多(P=0.003、P=0.002),视网膜组织水肿和空泡样状况改善,p-JNK、p-c-Jun、p-ERK、p-p38 MAPK和TNF-α蛋白表达明显减少(均为P0.05),且高剂量组的改善作用更为显著。结论白皮杉醇具有保护青光眼大鼠RGCs的作用,其作用机制可能与抑制MARK信号通路有关。
[Abstract]:Objective to observe the protective effect of taxol on retinal ganglion cells (retinal ganglion cells,RGCs) in glaucoma rats and to explore its possible mechanism. Methods Forty rats were randomly divided into control group, model group, low dose group and high dose group. The rat model of glaucoma was established by photocoagulation. The rats in the high dose group were given 100 mg kg~ (-1) and 200 mg kg~ (-1) pericycladol respectively. Intraocular pressure (IOP) was measured, RGCs was labeled with fluorescent gold retrograde and counted in each group. Retinal histopathology was observed by HE staining. Amino terminal kinase (phosphorylation c-Jun N-terminal kinase,p-JNK), phosphorylated c-Jun (phosphorylation c-junp-c-Jun), extracellular regulated protein kinase (phosphorylation extracellular regulated protein kinases,p-ERK), phosphorylated mitogen-activated protein (Jun (proto oncogene protein Jun,c-Jun), phosphorylated proto-oncogene protein (Jun (proto oncogene protein Jun,c-Jun), phosphorylated c-junp-c-Jun (phosphorylation c-junp-c-Jun), phosphorylated protein kinase (phosphorylation extracellular regulated protein kinases,p-ERK) in rat retina were detected by Western blot. Protein kinase p38 (phosphorylation mitogen activated protein kinases p-p38 MAPK and tumor necrosis factor- 伪 (tumor necrosis factor-alpha,TNF- 伪) were expressed. Results the intraocular pressure, the expression of p-JNK-c-Jun-p-ERKP p-p38 MAPK and TNF- 伪 protein in the model group were significantly higher than those in the control group (P0.05), RGCs, P0.05). Vacuoles and edema were observed in the retinal tissue structure. Compared with the model group, In the low dose group and the high dose group, the RGCs increased significantly (P0. 003 P0. 002), the retinal edema and cavitation status were improved, the expression of p-JNKP-c-Junp-ERKP-p38 MAPK and TNF- 伪 protein were significantly decreased (P0.05), and the improvement of high dose group was more significant. Conclusion Huperzine has protective effect on RGCs in glaucoma rats, and its mechanism may be related to the inhibition of MARK signaling pathway.
【作者单位】：南阳市中心医院眼科;第四军医大学西京医院眼科;南阳市唐河县城郊医院公共卫生服务科;
【分类号】：R775

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