双氢杨梅树皮素对人视网膜母细胞瘤细胞色素C氧化酶表达的影响

发布时间：2018-10-23 13:19

【摘要】：目的：测定有/无双氢杨梅树皮素（ampelopsin，APS）干预时视网膜母细胞瘤（retinoblastoma,RB）细胞中细胞色素C氧化酶（cytochrome coxidase,COX）蛋白的表达变化，探讨藤茶双氢杨梅树皮素对体外培养的人视网膜母细胞瘤细胞生长的影响及其抗肿瘤作用机制。方法：取处于对数生长期的人RB细胞株（HXO-RB44）进行体外培养，设置APS药物浓度三组：33.33（高）、14.84（中）、4.40（低）ug/mL，并设空白对照组及顺铂（2.00ug/mL）阳性对照组。在APS药物干预体外培养RB细胞24h后，采用WESTBOLT法测定视网膜母细胞瘤细胞中细胞色素C氧化酶蛋白IV亚基表达的灰度值，并求出相对灰度值。结果：不同药物浓度组APS干预体外培养的RB细胞24h后，，均能明显降低细胞色素C氧化酶IV亚基的活性表达，各APS药物组与空白对照组、阳性对照组比较，差异均有统计学意义，其中4.40ug/mL药物浓度组降低COX IV活性表达最为明显，相对灰度值为0.112±0.032。各药物组间并不呈现随药物浓度增加，COX IV活性表达降低越明显的特征。结论：1.APS能降低COX IV活性的表达，导致COX不能正常装配，功能下降，线粒体呼吸链电子传递受到抑制，进而引起线粒体损伤，激活肿瘤细胞的凋亡进程，达到抗肿瘤目的。2.COX IV活性的表达有其独特的调控机制，且可能存在启动阈值，其活性表达并不随APS药物浓度升高而降低。
[Abstract]:Objective: to determine the expression of cytochrome C oxidase (cytochrome coxidase,COX) protein in retinoblastoma (retinoblastoma,RB) cells treated with or without dihydromyricetin (ampelopsin,APS). To investigate the effect of Dihydromyricetin on the growth of human retinoblastoma cells in vitro and its antitumor mechanism. Methods: the human RB cell line (HXO-RB44) at logarithmic growth stage was cultured in vitro. The concentration of APS was set up in three groups: 33.33 (high), 14.84 (middle), 4.40 (low) ug/mL, blank control group and 2.00ug/mL positive control group. The expression of cytochrome C oxidase protein IV subunit in retinoblastoma cells was measured by WESTBOLT method after 24 hours of intervention by APS drugs, and the relative gray scale was obtained. Results: the activity of cytochrome C oxidase IV subunit was significantly decreased in RB cells cultured in vitro after 24 hours of intervention by APS in different concentration groups. The difference was statistically significant between each APS drug group and the blank control group and the positive control group. The activity of COX IV was significantly decreased in 4.40ug/mL group, with a relative gray value of 0.112 卤0.032. The expression of, COX IV activity decreased with the increase of drug concentration. Conclusion: 1.APS can reduce the expression of COX IV activity, leading to the COX not being assembled normally, the function decreasing, the electron transfer of mitochondrial respiratory chain being inhibited, and then causing mitochondrial damage and activating the apoptosis process of tumor cells. The expression of 2.COX IV activity has its own regulatory mechanism, and there may be a priming threshold. The expression of 2.COX IV activity does not decrease with the increase of APS concentration.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.7

【参考文献】