Nogo-A，NgR在大鼠视网膜缺血再灌注损伤中的表达
发布时间：2018-10-30 19:51
【摘要】：目的观察轴突生长抑制因子Nogo-A及其受体NgR在视网膜缺血再灌注(Retinal ischemia-reperfusion,RIR)急性损伤中的表达,研究两者在RIR损伤中的作用及相关性。方法90只SD大鼠随机分为：正常对照组(n=6只)；假手术组(n=42只)；RIR组(n=42只),假手术组及RIR组分为再灌注后0、6、12、24、48、72、168h亚组,每组6只；采用结扎单侧颈总动脉的方法制备大鼠RIR模型,HE染色观察组织形态学改变,免疫组织化学检测Nogo-A和NgR蛋白的表达。结果假手术组各时间点Nogo-A及NgR表达与正常组相比无差异(P0.05),实验组大鼠与假手术组相比：Nogo-A的表达在12h开始升高(P0.05),48h达到高峰(P0.01),72h下降(P0.05),168h达到正常基线水平(P0.05)；NgR的表达在6h即出现上升(P0.05),持续至48h达到最高峰(P0.01),72h下降(P0.05),168h达到正常基线水平(P0.05)。实验组大鼠Nogo-A与NgR的表达呈正相关(P0.01)。结论Nogo-A及NgR在RIR各时间点均有表达,其表达水平沿时间点呈抛物线形,在再灌注48h时均达到峰值,NgR先于Nogo-A表达,两者协同作用,与RIR损伤后抑制节细胞轴突修复再生相关。
[Abstract]:Objective to investigate the expression of axon growth inhibitor (Nogo-A) and its receptor NgR in acute retinal ischemia-reperfusion (Retinal ischemia-reperfusion,RIR) injury. Methods 90 SD rats were randomly divided into normal control group (n = 6), sham operation group (n = 42); RIR group (n = 42), sham operation group (n = 42) and RIR group (n = 6). The rat RIR model was established by ligating unilateral common carotid artery. The histomorphologic changes were observed by HE staining and the expression of Nogo-A and NgR proteins were detected by immunohistochemistry. Results there was no difference in the expression of Nogo-A and NgR between the sham operation group and the normal group (P0.05). Compared with the sham operation group, the expression of Nogo-A in the experimental group began to increase at 12 h (P0.05), and reached the peak at 48 h (P0.01). 72h decreased (P0.05), 168h reached the normal baseline level (P0.05); The expression of NgR increased at 6h (P0.05), reached its peak at 48h (P0.01), decreased at 72h (P0.05), and reached the normal baseline level at 168h (P0.05). There was a positive correlation between Nogo-A and NgR expression in experimental group (P0.01). Conclusion Nogo-A and NgR were expressed at each time point of RIR, and the expression level was parabola along the time point and reached its peak value at 48h after reperfusion. The expression of NgR was earlier than that of Nogo-A. It is related to the inhibition of axon repair and regeneration of ganglion cells after RIR injury.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R774.1
[Abstract]:Objective to investigate the expression of axon growth inhibitor (Nogo-A) and its receptor NgR in acute retinal ischemia-reperfusion (Retinal ischemia-reperfusion,RIR) injury. Methods 90 SD rats were randomly divided into normal control group (n = 6), sham operation group (n = 42); RIR group (n = 42), sham operation group (n = 42) and RIR group (n = 6). The rat RIR model was established by ligating unilateral common carotid artery. The histomorphologic changes were observed by HE staining and the expression of Nogo-A and NgR proteins were detected by immunohistochemistry. Results there was no difference in the expression of Nogo-A and NgR between the sham operation group and the normal group (P0.05). Compared with the sham operation group, the expression of Nogo-A in the experimental group began to increase at 12 h (P0.05), and reached the peak at 48 h (P0.01). 72h decreased (P0.05), 168h reached the normal baseline level (P0.05); The expression of NgR increased at 6h (P0.05), reached its peak at 48h (P0.01), decreased at 72h (P0.05), and reached the normal baseline level at 168h (P0.05). There was a positive correlation between Nogo-A and NgR expression in experimental group (P0.01). Conclusion Nogo-A and NgR were expressed at each time point of RIR, and the expression level was parabola along the time point and reached its peak value at 48h after reperfusion. The expression of NgR was earlier than that of Nogo-A. It is related to the inhibition of axon repair and regeneration of ganglion cells after RIR injury.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R774.1
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