兔慢性泪囊炎动物模型建立的初步研究
发布时间：2018-10-31 07:44
【摘要】：【目的】探索成功建立兔慢性泪囊炎动物模型的方法，分析成功造模的影响因素。 【方法】将55只兔随机分成A、B、C、D、E五组，每组11只，实验眼为右眼。A组暂时性泪道阻塞组，采用TDI榄橄油诱导过敏性鼻炎；B组单纯细菌接种组，由泪点注入金黄色葡萄球菌细菌悬液（107cells/ml）；C组永久性泪道阻塞组，由泪点注入MMA和PMMA混合物；D组永久性泪道阻塞组联合细菌接种组，即在C组基础上由泪道注入金黄色葡萄球菌细菌悬液（107cells/ml）；E组为正常对照组，由泪点注入注射无菌生理盐水。观察五组动物临床表现，进行泪道冲洗检查，将出现慢性泪囊炎临床表现泪道冲洗不通的兔，抽取泪囊分泌物细菌培养和鉴定、泪囊和鼻泪管组织常规病理学检查和超微病理学检查、泪道CT造影检查。统计慢性泪囊炎的发生率，分析慢性泪囊炎成功造模的影响因素。 【结果】A、B、C、D、E五组中，A、B、E组中所有兔均未出现慢性泪囊炎临床表现，泪道冲洗检查均通畅。C组和D组出现慢性泪囊炎临床表现的兔子分别为8只（成功率8/11，72.7%）和9只（9/11，81.8%），泪道冲洗均不通，多为造模后一周内开始出现流泪、分泌物增多等类似慢性泪囊炎的临床症状，且两组间出现慢性泪囊炎的时间上无显著差异。将出现慢性泪囊炎症状的上述兔的泪囊分泌物行细菌培养和鉴定结果、巴斯德杆菌的检出率为100%，且D组金黄色葡萄球菌为优势菌；泪囊和鼻泪管组织常规病理学表现为泪囊和鼻泪管管腔内有不同程度的渗出物，粘膜上皮细胞增生，泪囊粘膜上皮可见巢状的淋巴滤泡，上皮下弹力纤维和胶原纤维等出现变性，纤维结缔组织增生、排列紊乱，浆细胞等炎性细胞浸润，上皮下毛细血管管腔高度扩张充血；超微病理学表现鼻泪管粘膜柱状上皮细胞水肿，内有线粒体增生和内质网扩张，并可见细菌，，中性粒细胞浸润，泪囊粘膜上皮细胞水肿，细胞表面微绒毛减少，胞内可见炎性细胞浸润，鼻泪管粘膜下可见大量纤维结缔组织排列紊乱，纤维增生；泪囊粘膜下大量的炎性细胞浸润，主要为淋巴细胞、浆细胞和中性粒细胞；泪道CT造影清晰的显示出鼻泪管的阻塞部位以及阻塞程度。 【结论】单纯细菌接种和暂时性泪道阻塞动物模型不能出现慢性泪囊炎临床表现。而泪道永久性阻塞联合或不联合细菌接种动物模型中70%-80%可出现慢性泪囊炎临床表现，细菌培养阳性，常规病理学和超微病理学出现细胞结构及形态等的慢性炎症的改变，泪道CT造影显示鼻泪管阻塞，提示成功建立兔慢性泪囊炎动物模型，泪道完全性阻塞是慢性泪囊炎形成的基础条件。
[Abstract]:Objective: to explore the method of successful establishment of rabbit chronic dacryocystitis animal model and analyze the factors influencing the successful establishment of rabbit chronic dacryocystitis model. [methods] Fifty-five rabbits were randomly divided into five groups (n = 11 in each group) with right eyes, group A with temporary lacrimal duct obstruction and allergic rhinitis induced by TDI olive oil. Group B was inoculated with bacteria, and staphylococcus aureus suspension was injected with lacrimal point (107cells/ml); C group, permanent lacrimal duct obstruction group, MMA and PMMA mixture). Group D (permanent lacrimal passage obstruction) combined with bacterial inoculation group (group C) was injected with staphylococcus aureus bacteria suspension (107cells/ml); E group as normal control group, lacrimal point injection of sterile saline). The clinical manifestations of five groups of animals were observed and lacrimal duct irrigation was performed. The rabbits with chronic dacryocystitis were collected from the lacrimal sac secretion for bacterial culture and identification. Lacrimal sac and nasolacrimal duct histopathology and ultrastructural pathological examination, lacrimal duct CT examination. The incidence of chronic dacryocystitis and the influencing factors of successful modeling of chronic dacryocystitis were analyzed. [results] the clinical manifestations of chronic dacryocystitis were not found in all the rabbits in group A, B, C, D, E. The clinical manifestations of chronic dacryocystitis in group C and D were 8 (8 / 1172.7%) and 9 (9 / 1181.8%), respectively. Most of the clinical symptoms of chronic dacryocystitis appeared within one week after modeling, and there was no significant difference in the time of chronic dacryocystitis between the two groups. The lacrimal sac secretions of the rabbits with chronic dacryocystitis were cultured and identified. The detection rate of Pasteurella was 100, and group D was the dominant strain of Staphylococcus aureus. The histopathological manifestations of lacrimal sac and nasolacrimal duct were as follows: there were exudates in the lacrimal sac and nasolacrimal duct, mucosal epithelial cells proliferated, and nesting lymphoid follicles were found in dacryocyst mucosal epithelium. Degeneration of subepithelial elastic fibers and collagen fibers, proliferation of connective tissue, disorder of arrangement, infiltration of plasma cells and infiltration of inflammatory cells, hyperemia and hyperemia in capillaries. Ultrastructural pathology showed edema of columnar epithelial cells in nasolacrimal duct mucosa, mitochondria proliferation and endoplasmic reticulum dilatation, bacteria, neutrophil infiltration, edema of epithelial cells in dacryocyst mucosa, and decrease of microvilli on the surface of the cells. Inflammatory cell infiltration could be seen in the cells and a large number of fibrous connective tissues were found in the nasolacrimal duct. A large number of inflammatory cells infiltrated in the submucous membrane of the dacryocyst, mainly lymphocytes, plasma cells and neutrophils, and the location of obstruction and the degree of obstruction in the nasolacrimal duct were clearly revealed by lacrimal duct CT. [conclusion] chronic dacryocystitis can not appear in the animal model of bacterial inoculation and temporary lacrimal duct obstruction. In the animal model of permanent obstruction of lacrimal passage with or without bacterial inoculation, 70% to 80% of the patients had chronic dacryocystitis, and the bacteria culture was positive. The changes of chronic inflammation such as cell structure and morphology were observed in routine pathology and ultrapathology. Lacrimal passage CT showed obstruction of nasolacrimal duct, which suggested the successful establishment of animal model of chronic dacryocystitis in rabbits. Complete obstruction of lacrimal passage is the basic condition for the formation of chronic dacryocystitis.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R777.23
本文编号：2301423
[Abstract]:Objective: to explore the method of successful establishment of rabbit chronic dacryocystitis animal model and analyze the factors influencing the successful establishment of rabbit chronic dacryocystitis model. [methods] Fifty-five rabbits were randomly divided into five groups (n = 11 in each group) with right eyes, group A with temporary lacrimal duct obstruction and allergic rhinitis induced by TDI olive oil. Group B was inoculated with bacteria, and staphylococcus aureus suspension was injected with lacrimal point (107cells/ml); C group, permanent lacrimal duct obstruction group, MMA and PMMA mixture). Group D (permanent lacrimal passage obstruction) combined with bacterial inoculation group (group C) was injected with staphylococcus aureus bacteria suspension (107cells/ml); E group as normal control group, lacrimal point injection of sterile saline). The clinical manifestations of five groups of animals were observed and lacrimal duct irrigation was performed. The rabbits with chronic dacryocystitis were collected from the lacrimal sac secretion for bacterial culture and identification. Lacrimal sac and nasolacrimal duct histopathology and ultrastructural pathological examination, lacrimal duct CT examination. The incidence of chronic dacryocystitis and the influencing factors of successful modeling of chronic dacryocystitis were analyzed. [results] the clinical manifestations of chronic dacryocystitis were not found in all the rabbits in group A, B, C, D, E. The clinical manifestations of chronic dacryocystitis in group C and D were 8 (8 / 1172.7%) and 9 (9 / 1181.8%), respectively. Most of the clinical symptoms of chronic dacryocystitis appeared within one week after modeling, and there was no significant difference in the time of chronic dacryocystitis between the two groups. The lacrimal sac secretions of the rabbits with chronic dacryocystitis were cultured and identified. The detection rate of Pasteurella was 100, and group D was the dominant strain of Staphylococcus aureus. The histopathological manifestations of lacrimal sac and nasolacrimal duct were as follows: there were exudates in the lacrimal sac and nasolacrimal duct, mucosal epithelial cells proliferated, and nesting lymphoid follicles were found in dacryocyst mucosal epithelium. Degeneration of subepithelial elastic fibers and collagen fibers, proliferation of connective tissue, disorder of arrangement, infiltration of plasma cells and infiltration of inflammatory cells, hyperemia and hyperemia in capillaries. Ultrastructural pathology showed edema of columnar epithelial cells in nasolacrimal duct mucosa, mitochondria proliferation and endoplasmic reticulum dilatation, bacteria, neutrophil infiltration, edema of epithelial cells in dacryocyst mucosa, and decrease of microvilli on the surface of the cells. Inflammatory cell infiltration could be seen in the cells and a large number of fibrous connective tissues were found in the nasolacrimal duct. A large number of inflammatory cells infiltrated in the submucous membrane of the dacryocyst, mainly lymphocytes, plasma cells and neutrophils, and the location of obstruction and the degree of obstruction in the nasolacrimal duct were clearly revealed by lacrimal duct CT. [conclusion] chronic dacryocystitis can not appear in the animal model of bacterial inoculation and temporary lacrimal duct obstruction. In the animal model of permanent obstruction of lacrimal passage with or without bacterial inoculation, 70% to 80% of the patients had chronic dacryocystitis, and the bacteria culture was positive. The changes of chronic inflammation such as cell structure and morphology were observed in routine pathology and ultrapathology. Lacrimal passage CT showed obstruction of nasolacrimal duct, which suggested the successful establishment of animal model of chronic dacryocystitis in rabbits. Complete obstruction of lacrimal passage is the basic condition for the formation of chronic dacryocystitis.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R777.23
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本文编号：2301423
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