大鼠视盘周围神经纤维层厚度的光学相干断层扫描测量与组织学测量的相关性研究

发布时间：2018-11-02 15:14

【摘要】：目的：探讨正常以及视神经切断后成年大鼠视盘周围神经纤维层(retinal nerve fiber layer, RNFL)厚度的光学相干断层扫描(optical coherence tomography, OCT)测量与组织学测量结果的相关性。方法：采用OCT对36只大鼠(正常组20只；视神经切断模型组16只,分为1,3,5和7天存活组,每组4只)进行以视乳头为中心直径3.46mm圆环区域的视盘周围RNFL的厚度扫描,然后进行同一视网膜NF-200的免疫组织化学染色,测量相应部位的RNFL厚度。分别对西种方法测量的正常和模型组大鼠RNFL厚度数据进行线性回归统计分析。结果：OCT检测正常大鼠视盘周转RNFL厚度,右眼为72.35±5.71μm、左眼为72.65±5.88μm,双眼间差异无统计学意义(p0.05)；组织切片测量正常大鼠视盘周围RNFL厚度,右眼为37.54±4.05μm、左眼为37.38±4.23μm,双眼间差异无统计学意义(p0.05)；两种测量方法结果显示相关性较好(R2=0.8131)。在视神经切断模型组,两种方法的测量结果均显示视神经切断后大鼠视盘周围RNFL厚度值具有随着大鼠存活时间延长逐渐变小的趋势,且两种测量方法结果具有较好的相关性(R2=0.7964)。OCT测得的正常大鼠以及模型组大鼠视盘周围RNFL厚度明显高于组织学测量值。结论：采用OCT检测的视网膜神经纤维层厚度值可以代表该部位厚度的相对值,并且该厚度值与通过组织学方法获得的视网膜神经纤维层厚度值相关性很好。
[Abstract]:Aim: to investigate the correlation between optical coherence tomography (optical coherence tomography, OCT) and histological measurements of (retinal nerve fiber layer, RNFL) thickness of perioptic nerve fiber layer in normal and adult rats after optic nerve transection. Methods: 36 rats (20 normal rats) were treated with OCT. Sixteen rats in the optic nerve transection model group were divided into 5 and 7 days survival group (4 rats in each group). The thickness of RNFL around the optic disc with the optic papilla as the central diameter of the 3.46mm circle was scanned. The thickness of RNFL was measured by immunohistochemical staining of the same retinal NF-200. The RNFL thickness data of normal and model rats measured by Western method were analyzed by linear regression. Results: the thickness of RNFL was 72.35 卤5.71 渭 m in the right eye and 72.65 卤5.88 渭 m in the left eye. There was no significant difference between the two eyes (p0.05). The thickness of RNFL around optic disc in normal rats was 37.54 卤4.05 渭 m in right eye and 37.38 卤4.23 渭 m in left eye. In the optic nerve transection model group, the results of the two methods showed that the thickness of RNFL around the optic disc of the rats after optic nerve transection had a tendency to decrease with the prolongation of the survival time of the rats. There was a good correlation between the results of the two methods (the thickness of RNFL around the optic disc of normal rats and model rats measured by R2 + 0.7964). OCT) was significantly higher than that of histological measurement. Conclusion: the thickness of retinal nerve fiber layer measured by OCT can represent the relative value of the thickness of the retinal nerve fiber layer, and it has a good correlation with the thickness of retinal nerve fiber layer obtained by histological method.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R774.6

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