鼻息肉组织中白介素-20、白介素-22的表达

发布时间：2018-11-04 08:08

【摘要】：目的:研究IL-20与IL-22在鼻息肉患者的息肉组织以及正常鼻黏膜组织中的表达及鼻息肉组织中IL-20、IL-22表达的相关性,分析其在鼻息肉的发生、进展及疾病复发中的作用机制,为鼻息肉发生机制的科学研究奠定实验基础,旨在以IL-20、IL-22为靶点治疗鼻息肉并防止其复发。方法:1.本实验收集于2015.11-2016.4在山西医科大学第一医院耳鼻喉科手术的患者,实验组为鼻息肉患者,共50例,在其行功能性鼻内镜手术时,取鼻息肉的头端组织;对照组为鼻中隔偏曲的患者,共50例,取其行鼻中隔偏曲矫正术对侧代偿性肥大的下鼻甲黏膜组织。2.实验方法采取免疫组化法SP6001,测定鼻息肉组织及正常鼻黏膜组织中IL-20与IL-22的表达情况。3.IL-20和IL-22表达的免疫组化评分标准,根据某一高倍镜视野下细胞的阳性率和细胞的显色程度:若无染色或无明显染色记为0分;若阳性染色细胞数㩳10%记1分;阳性细胞数大于等于10%但低于40%记为2分;阳性细胞数大于等于40%但小于70%记为3分;阳性细胞数大于等于70%记为4分;无染色或无明显染色记为0分;浅黄色记为1分;浅黄色至黄色记为2分;黄色至棕黄色记为3分;棕黄色至褐色记为4分。最后将上述两种测定结果的评分的平均值加起来便是该组织玻片的最后得分。评分大于等于0分小于2分记为阴性(-);大于等于2分小于4分记为弱阳性(+);大于等于4分小于6分记为阳性(++);大于等于6分记为强阳性(+++)。4.所得数据采用SPSS21.0软件进行统计学分析。结果:1.IL-20、IL-22在鼻息肉组织及正常鼻黏膜组织中均有表达,表达结果分为阴性、弱阳性、阳性、强阳性,IL-20在鼻息肉组织中的表达4组结果分别有2、10、19、19例,IL-22则分别有0、14、23、13例;IL-20在正常鼻黏膜组织中的表达4组结果分别有4、17、22、7例,IL-22则分别有2、20、23、5例。2.IL-20和IL-22分别在鼻息肉组织和正常鼻黏膜组织中的表达存在显著差异(p㩳0.05),IL-20和IL-22在鼻息肉组织中的表达均明显高于对照组。3.在鼻息肉组织中,IL-20和IL-22的表达具有相关性(p㩳0.05),呈正相关关系。结论:IL-20、IL-22作为炎性细胞因子,在鼻息肉组织中的表达明显升高,且表达呈正相关关系,提示IL-20、IL-22在鼻息肉中的表达具有相互促进作用,共同促进鼻息肉的发病及进展。
[Abstract]:Objective: to study the expression of IL-20 and IL-22 in polyps of nasal polyps, normal nasal mucosa and the expression of IL-20,IL-22 in nasal polyps, and to analyze the occurrence of IL-20,IL-22 in nasal polyps. The progress and the mechanism of disease recurrence lay an experimental foundation for the scientific study of the mechanism of nasal polyps. The aim of this study is to treat nasal polyps with IL-20,IL-22 as a target and prevent their recurrence. Methods: 1. In this experiment, 50 cases of nasal polyps were collected in the otolaryngology department of the first Hospital of Shanxi Medical University from May 11 to June 2014.The nasal polyps were removed from the head of the nasal polyps during the functional endoscopic sinus surgery. In the control group, 50 patients with nasal septum deviation were selected. The mucosa of inferior turbinate with contralateral compensatory hypertrophy was obtained by correction of nasal septum deviation. 2. Methods the expression of IL-20 and IL-22 in nasal polyps and normal nasal mucosa was detected by immunohistochemical SP6001, method. The standard of immunohistochemistry for 3.IL-20 and IL-22 expression was used. According to the positive rate of cells and the color degree of cells under a high power microscope, if there was no staining or no obvious staining, the score was 0; If the number of positive staining cells is 10% and 1, the number of positive cells > 10% but less than 40% is 2 points, the number of positive cells > 40% but less than 70% is 3 points, the number of positive cells more than 70% is 4 points, the number of positive cells more than 70% is equal to 70%, and the number of positive cells is more than 70%. No staining or no obvious staining: 0; light yellow 1; light yellow to yellow 2; yellow to brown 3; brown to brown 4. The final score of the tissue slide was calculated by adding the average score of the above two measurements. Scores greater than or equal to 0 < 2 are negative (-), > 2 < 4 are recorded as weak positive (); > or equal to 4 < 6, positive (); is greater than or equal to 6, and strong positive (). 4 is recorded as greater than or equal to 6. The data were analyzed by SPSS21.0 software. Results: 1.The expression of IL-20 IL-22 in nasal polyps and normal nasal mucosa was negative, weakly positive and strongly positive. The expression of IL-20 IL-22 in nasal polyps was 2101919 cases in 4 groups, respectively. In IL-22, there were 14 cases and 2313 cases, respectively. The expression of IL-20 in normal nasal mucosa in 4 groups were 41717 cases and 227 cases, respectively. The expression of 2.IL-20 and IL-22 in nasal polyps and normal nasal mucosa were significantly different (p0. 05). The expression of IL-20 and IL-22 in nasal polyps was significantly higher than that in control group. In nasal polyps, the expression of IL-20 and IL-22 was positively correlated (p0. 05). Conclusion: as an inflammatory cytokine, the expression of IL-20,IL-22 in nasal polyps is significantly increased, and the expression of IL-20,IL-22 is positively correlated, suggesting that the expression of IL-20,IL-22 in nasal polyps can promote each other. To promote the development of nasal polyps.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R765.25

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