运动干预对胎鼠前庭器官发育的影响
发布时间：2018-11-18 13:29
【摘要】：在妊娠的不同时间段内，孕妇做何种方式的运动、多大的运动强度、多长的持续运动时间对胎儿的发育和孕妇的健康是最佳的？在世界范围内，至今还没有一个以基础研究做基础的科学指导原则。前庭感觉是胚胎发育过程中较早形成的感觉，仅次于触觉，其发育的迟缓或缺如可严重影响视觉、听觉的发育。如导致前庭-眼反射障碍，影响将来的阅读能力等。在长期进化过程中，运动和环境已成为胚胎发育和生后发育必不可少的刺激诱导因素。由于现代人运动方式发生改变、运动强度降低，尤其在胚胎发育期，导致运动相关的基因并未按程序进行充分的表达，造成发育不足、运动能力下降。有文献报道，对孕妇的跳舞训练能提高子体的运动协调能力，，这表明在胚胎发育期适度地增加运动干预对前庭系统的发育具有一定的促进作用。那么这种运动干预的时机、强度、持续时间，以及促进发育的机制，都是亟待深入研究的课题。 本论文拟以妊娠小鼠为对象，在不同妊娠期内给予小强度的运动干预，用EdU标记的方法观察胎鼠前庭感觉上皮细胞发育状况；用行为学方法检测子鼠出生后的前庭功能和对高强度运动训练的习服能力；以免疫组化方法检测子鼠出生当天的前庭器官的传出神经支配。通过这些研究以期确定妊娠期的运动干预是否能提高子鼠的前庭功能和对高强度运动训练的习服能力？是否存在一个关键期？子鼠前庭功能的的提高是否与前庭感觉上皮细胞、神经支配发育较早有关？经过实验得到如下结果： 1.各组每窝产仔数、窝平均体重、张耳和睁眼天数之间没有显著差异； 2. E10-E15组平面翻正反射达标率显著高于其他各组； 3. PD13和PD14天E10-E15组子鼠空中翻正反射平均得分明显高于对照组，且差异显著； 4.在抗运动病能力实验中，自主活动站立次数对照组一直低于各旋转训练组，且在一天测试天内呈现显著性差异，其他测试指标如粪便粒数、自主活动次数、糖精水各组之间没有差异。 5．EdU标记方法得出实验组和对照组的球囊和椭圆囊感觉上皮细胞的增殖数量之间没有显著差异； 6.免疫组化方法得出CGRP能传出纤维实验组和对照组在纤维分布的位置和数量上无显著性差异。 通过对实验结果的分析得出如下结论： 1.所用运动训练强度和时间对每窝胎鼠的只数、胎鼠整体发育和子鼠成年后对高强度运动训练的习服能力没有显著影响； 2.这种运动训练能显著提高子鼠的前庭功能，最佳运动干预期为小鼠胚胎发育的第10-15天，此期恰为前庭器官原基形成至毛细胞分化的时期； 3.对毛细胞的增殖和传出神经支配没有显著影响。 本结果为进一步探索促进前庭系统发育的、适于孕妇运动的方式、强度和时间奠定了基础，为科学地指导孕妇的运动训练提供了理论依据。
[Abstract]:In the different period of pregnancy, what kind of exercise does the pregnant woman do, how much exercise intensity, how long the sustained exercise time to the fetus development and the pregnant woman's health is the best? There is no scientific guiding principle based on basic research in the world. Vestibular sensation is the sense formed earlier in the embryonic development, second only to the tactile sense, and its developmental retardation or absence can seriously affect the development of vision and hearing. If causes vestibular-eye reflex disorder, affects the future reading ability and so on. During the long-term evolution, exercise and environment have become the essential stimuli and inducers of embryonic and postnatal development. Due to the change of modern exercise mode and the decrease of exercise intensity, especially in the embryonic development stage, the exercise related genes are not fully expressed according to the procedure, resulting in underdevelopment and decreased motor ability. It has been reported that dance training for pregnant women can improve the motor coordination ability of their offspring, which indicates that moderate increase of exercise intervention during embryonic development can promote the development of vestibular system. The timing, intensity, duration and developmental mechanism of this exercise intervention are urgent to be studied. In this study, pregnant mice were given small intensity exercise intervention during different gestation periods, and the development of vestibular sensory epithelial cells in fetal mice was observed by EdU labeling method. The vestibular function after birth and acclimatization ability of high-intensity exercise training were detected by behavioral method and the efferent innervation of vestibular organ was detected by immunohistochemical method. Through these studies, we hope to determine whether the exercise intervention during pregnancy can improve the vestibular function and acclimatization of high-intensity exercise training. Is there a critical period? Is the improvement of vestibular function related to the earlier development of vestibular sensory epithelial cells and innervation? The experimental results are as follows: 1. There was no significant difference in litter size, litter weight, ear opening and eye opening days in each group. The reaching rate of plane positive reflex in E10-E15 group was significantly higher than that in other groups. The average score of aerial positive reflex in E10-E15 group on PD13 and PD14 days was significantly higher than that in control group, and the difference was significant. 4. In the exercise sickness resistance experiment, the standing times of the control group was lower than that of the rotation training group, and there was significant difference in the day of test. Other test indexes such as fecal granule number, number of autonomous activities, There was no difference between the saccharin water groups. 5.EdU labeling method showed that there was no significant difference in the number of sensory epithelial cells proliferation between the experimental group and the control group. Immunohistochemical method showed that there was no significant difference in the distribution and quantity of CGRP efferent fibers between experimental group and control group. Through the analysis of the experimental results, the following conclusions are obtained: 1. The intensity and time of exercise training had no significant effect on the number of fetal mice per litter, the overall development of fetal mice and the acclimatization ability of offspring after adulthood. 2. This exercise can significantly improve the vestibular function of the offspring. The best exercise stem is expected to be the 10-15 days of mouse embryonic development, which is the period of vestibular organ primordium formation to hair cell differentiation; 3. There was no significant effect on hair cell proliferation and efferent innervation. The results laid a foundation for further exploration of the mode, intensity and time of maternal exercise which can promote the development of vestibular system and provide a theoretical basis for scientifically guiding the exercise training of pregnant women.
【学位授予单位】：河北师范大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R764
本文编号：2340156
[Abstract]:In the different period of pregnancy, what kind of exercise does the pregnant woman do, how much exercise intensity, how long the sustained exercise time to the fetus development and the pregnant woman's health is the best? There is no scientific guiding principle based on basic research in the world. Vestibular sensation is the sense formed earlier in the embryonic development, second only to the tactile sense, and its developmental retardation or absence can seriously affect the development of vision and hearing. If causes vestibular-eye reflex disorder, affects the future reading ability and so on. During the long-term evolution, exercise and environment have become the essential stimuli and inducers of embryonic and postnatal development. Due to the change of modern exercise mode and the decrease of exercise intensity, especially in the embryonic development stage, the exercise related genes are not fully expressed according to the procedure, resulting in underdevelopment and decreased motor ability. It has been reported that dance training for pregnant women can improve the motor coordination ability of their offspring, which indicates that moderate increase of exercise intervention during embryonic development can promote the development of vestibular system. The timing, intensity, duration and developmental mechanism of this exercise intervention are urgent to be studied. In this study, pregnant mice were given small intensity exercise intervention during different gestation periods, and the development of vestibular sensory epithelial cells in fetal mice was observed by EdU labeling method. The vestibular function after birth and acclimatization ability of high-intensity exercise training were detected by behavioral method and the efferent innervation of vestibular organ was detected by immunohistochemical method. Through these studies, we hope to determine whether the exercise intervention during pregnancy can improve the vestibular function and acclimatization of high-intensity exercise training. Is there a critical period? Is the improvement of vestibular function related to the earlier development of vestibular sensory epithelial cells and innervation? The experimental results are as follows: 1. There was no significant difference in litter size, litter weight, ear opening and eye opening days in each group. The reaching rate of plane positive reflex in E10-E15 group was significantly higher than that in other groups. The average score of aerial positive reflex in E10-E15 group on PD13 and PD14 days was significantly higher than that in control group, and the difference was significant. 4. In the exercise sickness resistance experiment, the standing times of the control group was lower than that of the rotation training group, and there was significant difference in the day of test. Other test indexes such as fecal granule number, number of autonomous activities, There was no difference between the saccharin water groups. 5.EdU labeling method showed that there was no significant difference in the number of sensory epithelial cells proliferation between the experimental group and the control group. Immunohistochemical method showed that there was no significant difference in the distribution and quantity of CGRP efferent fibers between experimental group and control group. Through the analysis of the experimental results, the following conclusions are obtained: 1. The intensity and time of exercise training had no significant effect on the number of fetal mice per litter, the overall development of fetal mice and the acclimatization ability of offspring after adulthood. 2. This exercise can significantly improve the vestibular function of the offspring. The best exercise stem is expected to be the 10-15 days of mouse embryonic development, which is the period of vestibular organ primordium formation to hair cell differentiation; 3. There was no significant effect on hair cell proliferation and efferent innervation. The results laid a foundation for further exploration of the mode, intensity and time of maternal exercise which can promote the development of vestibular system and provide a theoretical basis for scientifically guiding the exercise training of pregnant women.
【学位授予单位】：河北师范大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R764
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相关期刊论文 前3条
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