过敏性鼻炎患者血液代谢组学研究

发布时间：2018-11-23 09:56

【摘要】：目的:采用代谢组学方法分析过敏性鼻炎患者血清中小分子代谢物的变化。方法:采用气相色谱-质谱联用技术(GC-MS)检测20例过敏性鼻炎患者(病例)和同期20例健康者(对照组)血清中代谢物,采用正交偏最小二乘法(OPLS)的方法,观察病例组和对照组的代谢谱变化,并寻找有差异的小分子代谢物。结果:对照组和病例组的代谢谱能明显区分,鉴定了23个差异代谢物,并对差异代谢物进行了ROC曲线绘制,计算ROC曲线下的面积,得出曲线下面积大于0.5的物质有甘油酸、丝氨酸、苏氨酸、甲硫氨酸、半胱氨酸、天冬酰胺、谷氨酰胺、色氨酸和十九烷酸。结合代谢途径分析,发现过敏性鼻炎患者体内存在丙氨酸、天冬氨酸和谷氨酸代谢,甘氨酸、丝氨酸和苏氨酸代谢,半胱氨酸和甲硫氨酸代谢和色氨酸代谢异常。结论:基于GC/MS的代谢组学技术能全面客观地反映过敏性鼻炎患者体内血液中的代谢物变化,小分子代谢物分析及代谢途径探讨将为过敏性鼻炎的诊断和发病机制研究提供一个可借鉴的思路和手段。
[Abstract]:Objective: to analyze the changes of small molecule metabolites in serum of allergic rhinitis patients by metabonomics. Methods: serum metabolites were detected by gas chromatography-mass spectrometry (GC-MS) in 20 patients with allergic rhinitis (cases) and 20 healthy subjects (control group) at the same time. Orthogonal partial least square method (OPLS) was used to detect the metabolites in serum of 20 patients with allergic rhinitis. The changes of metabolic spectrum were observed in case group and control group, and small molecular metabolites were found. Results: the metabolic spectra of the control group and the case group could be distinguished obviously, 23 different metabolites were identified, and the ROC curve of the differential metabolites was plotted. The area under the ROC curve was calculated. The results showed that the area under the curve was more than 0.5 with glyceric acid. Serine, threonine, methionine, cysteine, asparagine, glutamine, tryptophan and heptadecanoic acid. Combined with metabolic pathway analysis, it was found that there were alanine, aspartic acid and glutamate metabolism, glycine, serine and threonine metabolism, cysteine and methionine metabolism and tryptophan metabolism in patients with allergic rhinitis. Conclusion: the metabolic technique based on GC/MS can comprehensively and objectively reflect the changes of metabolites in the blood of allergic rhinitis patients. The analysis of small molecule metabolites and the discussion of metabolic pathway will provide a useful method for the diagnosis and pathogenesis of allergic rhinitis.
【作者单位】：上海市宝山区中西医结合医院中心实验室;上海中医药大学附属曙光医院宝山分院针灸科;
【基金】：国家自然科学基金项目(编号:81473475) 宝山区科技创新专项基金项目(编号:13-E-18)
【分类号】：R765.21

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