长期注射水杨酸钠大鼠下丘EphA4的表达
发布时间：2018-12-08 15:58
【摘要】：目的通过观察长期水杨酸钠作用后大鼠ABR及下丘EphA4mRNA表达变化,探讨下丘EphA4表达在水杨酸钠耳毒性中的作用。方法将30只健康成年雄性SD大鼠随机分为5组,每组6只:正常对照组(不予任何处理),肌注7天组(肌肉注射水杨酸钠175mg/kg,每天2次,间隔8小时,连续7天),肌注14天组(水杨酸钠用法同前,持续14天),恢复14天组(肌注水杨酸钠14天后,停药恢复14天),恢复28天组(肌注水杨酸钠14天后,停药恢复28天)。对各组大鼠分别于相应时间点进行ABR检测后处死并迅速分离下丘,采用实时荧光定量PCR(real-time PCR)的方法检测各组大鼠下丘中EphA4mRNA表达。结果 1肌注7、14天组大鼠的ABR反应阈分别为38.33±3.73、44.16±1.86dB SPL,较对照组(30.83±1.86dB SPL)明显升高(P0.05);恢复28天组大鼠的ABR反应阈(32.50±2.50dB SPL)与对照组(30.83±1.86dB SPL)相比,差异无明显统计学意义(P0.05);2肌注7天组和恢复14天组大鼠下丘EphA4mRNA表达(分别为:0.69±0.11、0.67±0.09)均低于对照组大鼠(0.99±0.01)(均为P0.05);恢复28天组大鼠下丘EphA4mRNA表达(0.88±0.04)与对照组大鼠比较差异无统计学意义(P0.05)。结论长期注射水杨酸钠后大鼠下丘的EphA4表达呈可逆性下降,且与水杨酸钠注射后大鼠听功能损伤的变化一致,推测下丘神经元轴突中EphA4参与了水杨酸钠耳毒性的机制。
[Abstract]:Objective to investigate the role of EphA4 expression in sodium salicylate ototoxicity by observing the changes of EphA4mRNA expression in ABR and inferior colliculus after long-term sodium salicylate treatment in rats. Methods Thirty healthy adult male SD rats were randomly divided into 5 groups, 6 rats in each group: normal control group (no treatment), intramuscular injection of sodium salicylate (175 mg / kg, twice a day, 8 hours interval for 7 days), intramuscular injection of sodium salicylate (175mg / kg), intramuscular injection of sodium salicylate (175mg / kg). The rats were given intramuscular injection of sodium salicylate for 14 days (sodium salicylate for 14 days), recovery group for 14 days after sodium salicylate injection for 14 days, and group for 28 days after sodium salicylate was injected intramuscularly. The rats in each group were killed after ABR detection at the corresponding time points and the expression of EphA4mRNA in the inferior colliculus was detected by real-time fluorescence quantitative PCR (real-time PCR). Results 1the ABR response threshold of the rats in the group of intramuscular injection for 14 days was 38.33 卤3.73 卤44.16 卤1.86dB SPL, which was significantly higher than that in the control group (30.83 卤1.86dB SPL) (P0.05). There was no significant difference in ABR response threshold (32.50 卤2.50dB SPL) compared with control group (30.83 卤1.86dB SPL) after 28 days of recovery (P0.05). 2the expression of EphA4mRNA in the inferior colliculus of the rats in the 7-day group and the 14-day group (0.69 卤0.110.67 卤0.09, respectively) was lower than that in the control group (0.99 卤0.01) (P0.05); The expression of EphA4mRNA in inferior colliculus of rats in 28 days group (0.88 卤0.04) was not significantly different from that in control group (P0.05). Conclusion the expression of EphA4 in the inferior colliculus of rats after long-term injection of sodium salicylate was inversely decreased, which was consistent with the changes of auditory function of rats after sodium salicylate injection. It is speculated that EphA4 in axons of inferior colliculus neurons may play a role in the mechanism of sodium salicylate ototoxicity.
【作者单位】： 武汉大学人民医院耳鼻咽喉头颈外科;
【基金】：国家自然科学基金青年基金(81100712) 湖北省自然科学基金面上项目(220100653)资助
【分类号】：R764
本文编号：2368610
[Abstract]:Objective to investigate the role of EphA4 expression in sodium salicylate ototoxicity by observing the changes of EphA4mRNA expression in ABR and inferior colliculus after long-term sodium salicylate treatment in rats. Methods Thirty healthy adult male SD rats were randomly divided into 5 groups, 6 rats in each group: normal control group (no treatment), intramuscular injection of sodium salicylate (175 mg / kg, twice a day, 8 hours interval for 7 days), intramuscular injection of sodium salicylate (175mg / kg), intramuscular injection of sodium salicylate (175mg / kg). The rats were given intramuscular injection of sodium salicylate for 14 days (sodium salicylate for 14 days), recovery group for 14 days after sodium salicylate injection for 14 days, and group for 28 days after sodium salicylate was injected intramuscularly. The rats in each group were killed after ABR detection at the corresponding time points and the expression of EphA4mRNA in the inferior colliculus was detected by real-time fluorescence quantitative PCR (real-time PCR). Results 1the ABR response threshold of the rats in the group of intramuscular injection for 14 days was 38.33 卤3.73 卤44.16 卤1.86dB SPL, which was significantly higher than that in the control group (30.83 卤1.86dB SPL) (P0.05). There was no significant difference in ABR response threshold (32.50 卤2.50dB SPL) compared with control group (30.83 卤1.86dB SPL) after 28 days of recovery (P0.05). 2the expression of EphA4mRNA in the inferior colliculus of the rats in the 7-day group and the 14-day group (0.69 卤0.110.67 卤0.09, respectively) was lower than that in the control group (0.99 卤0.01) (P0.05); The expression of EphA4mRNA in inferior colliculus of rats in 28 days group (0.88 卤0.04) was not significantly different from that in control group (P0.05). Conclusion the expression of EphA4 in the inferior colliculus of rats after long-term injection of sodium salicylate was inversely decreased, which was consistent with the changes of auditory function of rats after sodium salicylate injection. It is speculated that EphA4 in axons of inferior colliculus neurons may play a role in the mechanism of sodium salicylate ototoxicity.
【作者单位】： 武汉大学人民医院耳鼻咽喉头颈外科;
【基金】：国家自然科学基金青年基金(81100712) 湖北省自然科学基金面上项目(220100653)资助
【分类号】：R764
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