CRF在鼻部的表达及其与上气道炎症的关系

发布时间：2018-12-17 03:27

【摘要】：目的：气道炎症是涉及多种炎症细胞和炎症介质的慢性免疫性疾病,目前对气道炎症的治疗不理想,因此对其发病机制的进一步研究以及寻找新的药物治疗、预防疾病是非常有意义的。CRF在外周炎症中发挥重要的作用,本试验的目的是通过研究CRF在上气道炎症患者鼻组织中的表达情况以及与嗜酸性粒细胞、肥大细胞的相关性,探讨CRF在气道炎症性疾病中的作用。方法：标本均来自住院行鼻内镜手术的患者：变应性鼻炎合并鼻息肉组10例、变应性鼻炎组9例和正常对照组7例,鼻息肉组收集鼻息肉标本,变应性鼻炎组和正常对照组则收集下鼻甲标本。行免疫组化染色和图像分析,检测CRF在组织中的表达情况；行HE染色、甲苯胺蓝改良染色以计数嗜酸性粒细胞、肥大细胞；分析CRF在各组中的的表达以及与肥大细胞、嗜酸性粒细胞的相关性。结果：嗜酸性粒细胞、肥大细胞的数量在变应性鼻炎组(13.90±3.08,10.22±2.96)及鼻息肉组(12.59±2.69,6.23±3.23)明显高于对照组(7.33±4.18,4.48±2.38),差异具有统计学意义(P0.05)；CRF的光密度值在变应性鼻炎组(0.273±0.028)及鼻息肉组(0.264±0.019)中明显高于正常对照组(0.229±0.015),差异具有统计学意义(P0.01),然而CRF在变应性鼻炎组和变应性鼻炎合并鼻息肉组两组间差异无显著性；CRF在鼻部的表达与肥大细胞、嗜酸性粒细胞的数量均呈正相关(r分别为0.732,0.582)。结论：(1).CRF在鼻部粘膜表达,可能参与了变应性鼻炎与鼻息肉的鼻黏膜慢性炎症反应过程；(2).肥大细胞、嗜酸性粒细胞可能是鼻部CRF的主要来源；(3).CRF在上气道炎症性疾病中的表达及其与肥大细胞、嗜酸性粒细胞之间的联系,为今后寻找新的治疗方法提供了理论思路。
[Abstract]:Objective: airway inflammation is a chronic immune disease involving many inflammatory cells and inflammatory mediators. At present, the treatment of airway inflammation is not ideal. CRF plays an important role in peripheral inflammation. The purpose of this study was to investigate the expression of CRF in nasal tissues of patients with upper airway inflammation and its association with eosinophils and mast cells. To investigate the role of CRF in airway inflammatory diseases. Methods: the specimens were collected from 10 cases of allergic rhinitis complicated with nasal polyps, 9 cases of allergic rhinitis group and 7 cases of normal control group. Specimens of inferior turbinate were collected from allergic rhinitis group and normal control group. Immunohistochemical staining and image analysis were performed to detect the expression of CRF in tissues, HE staining and toluidine blue modified staining were performed to count eosinophils and mast cells. The expression of CRF in each group and its correlation with mast cells and eosinophils were analyzed. Results: the number of eosinophilic granulocytes and mast cells in allergic rhinitis group (13.90 卤3.08 卤10.22 卤2.96) and nasal polyp group (12.59 卤2.69 卤6.23 卤3.23) was significantly higher than that in control group (7.33 卤4.184.48 卤2.38). The difference was statistically significant (P0.05). The optical density of CRF in allergic rhinitis group (0.273 卤0.028) and nasal polyp group (0.264 卤0.019) was significantly higher than that in normal control group (0.229 卤0.015). However, there was no significant difference in CRF between allergic rhinitis group and allergic rhinitis with nasal polyp group. The expression of CRF in the nose was positively correlated with the number of mast cells and eosinophils (r = 0.732 卤0.582, respectively). Conclusion: (1) the expression of). CRF in nasal mucosa may be involved in the process of chronic inflammation of nasal mucosa in allergic rhinitis and nasal polyps; (2). Mast cells and eosinophilic granulocytes may be the main sources of nasal CRF. (3) the expression of). CRF in inflammatory diseases of upper airway and its relationship with mast cells and eosinophils, which provides a theoretical idea for finding new treatment methods in the future.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R765

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