iNKT细胞与儿童分泌性中耳炎相关性的初步探讨
发布时间：2018-12-23 12:08
【摘要】：目的为了探讨iNKT细胞是否参与了分泌性中耳炎的发生，本文采用流式细胞术，对分泌性中耳炎患儿外周血、扁桃体组织、腺样体组织内iNKT细胞进行检测，探讨用anti-humanCD3-FITC和anti-human6B11-PE标记iNKT细胞的可行性，首次分析iNKT细胞与儿童分泌性中耳炎的关系。 方法分别收集正常儿童外周血10例，不伴SOM的慢性扁桃体炎和腺样体肥大患儿的外周血、扁桃体、腺样体组织各13例，以及分泌性中耳炎患儿外周血13例、扁桃体组织7例、腺样体组织12例。用特异性抗体anti-human CD3-FITC和anti-human6B11-PE标记外周血和组织中iNKT细胞，利用流式细胞学术（FCM）检测iNKT细胞在各标本的百分比，并比较各组间是否存在差异。 结果1外周血iNKT细胞的检测结果 正常儿童外周血iNKT细胞的比例为（0.9660±0.4221）%；不伴SOM的慢性扁桃体炎和腺样体肥大患儿外周血iNKT细胞的比例为（1.5231±1.0226）%；分泌性中耳炎患儿外周血iNKT细胞的比例为（1.5692±0.7200）%;经单因素的方差分析，计算得P＜0.05，，三组间的差异有统计学意义，经LSD两两比较，正常儿童与分泌性中耳炎患儿差异显著，其他两组间差异无统计学意义。 2扁桃体组织iNKT细胞的检测结果 分泌性中耳炎患儿扁桃体组织iNKT细胞的比例为（1.0105±0.5241）%；不伴SOM的慢性扁桃体炎患儿扁桃体组织iNKT细胞的比例为（0.8883±0.6419）%；经两独立样本的t检验，计算得P＞0.05，两组间差异无统计学意义。 3腺样体组织iNKT细胞检测结果 分泌性中耳炎患儿腺样体组织iNKT细胞的比例为（1.2658±0.8250）%；不伴SOM的腺样体肥大患儿腺样体组织iNKT细胞的比例为（0.8246±0.4289）%；经两独立样本t检验，计算得P＞0.05，两组间差异无统计学意义。 结论用anti-human CD3-FITC和anti-human6B11-PE标记iNKT细胞可行。在SOM患儿与不伴SOM的慢性扁桃体炎、腺样体肥大患儿的扁桃体、腺样体组织中，iNKT细胞比例差异无统计学意义。分泌性中耳炎患儿外周血中iNKT细胞的比例比正常儿童高，初步推测iNKT细胞与儿童分泌性中耳炎的发生可能有关。
[Abstract]:Objective to investigate whether iNKT cells are involved in the occurrence of secretory otitis media, iNKT cells in peripheral blood, tonsils and adenoids of children with secretory otitis media were detected by flow cytometry. To explore the feasibility of labeling iNKT cells with anti-humanCD3-FITC and anti-human6B11-PE, the relationship between iNKT cells and secretory otitis media in children was analyzed for the first time. Methods the peripheral blood, tonsillar and adenoid tissues of 10 normal children, 13 children with chronic tonsillitis and adenoid hypertrophy without SOM, 13 patients with secretory otitis media and 7 patients with tonsillar tissue were collected. Adenoid tissue in 12 cases. Specific antibody anti-human CD3-FITC and anti-human6B11-PE were used to label iNKT cells in peripheral blood and tissues. The percentage of iNKT cells in each specimen was detected by flow cytometry academic (FCM). Results 1 the percentage of iNKT cells in peripheral blood of normal children was (0.9660 卤0.4221)%, that of children without SOM was (1.5231 卤1.0226)%, that of children with chronic tonsillitis and adenoid hypertrophy was (1.5231 卤1.0226)%. The percentage of iNKT cells in peripheral blood of children with secretory otitis media was (1.5692 卤0.7200)%. By single factor analysis of variance, P < 0.05, the difference among the three groups was statistically significant. By LSD, there was significant difference between normal children and children with secretory otitis media, but there was no significant difference between the other two groups. 2Detection of iNKT cells in tonsils the percentage of iNKT cells in tonsils of children with secretory otitis media was (1.0105 卤0.5241)%. The percentage of iNKT cells in tonsil tissue of children with chronic tonsillitis without SOM was (0.8883 卤0.6419)%, which was calculated by t-test of two independent samples (P > 0.05), and there was no significant difference between the two groups. 3The percentage of adenoid tissue iNKT cells was (1.2658 卤0.8250)% in children with secretory otitis media and (0.8246 卤0.4289)% in adenoid tissues without SOM. After two independent samples t test, P > 0.05, there was no significant difference between the two groups. Conclusion it is feasible to label iNKT cells with anti-human CD3-FITC and anti-human6B11-PE. There was no significant difference in the proportion of iNKT cells in tonsils and adenoids between children with SOM and those with chronic tonsillitis without SOM and adenoid hypertrophy. The percentage of iNKT cells in peripheral blood of children with secretory otitis media was higher than that of normal children. It was suggested that iNKT cells might be related to the occurrence of secretory otitis media in children.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R764.21
本文编号：2389964
[Abstract]:Objective to investigate whether iNKT cells are involved in the occurrence of secretory otitis media, iNKT cells in peripheral blood, tonsils and adenoids of children with secretory otitis media were detected by flow cytometry. To explore the feasibility of labeling iNKT cells with anti-humanCD3-FITC and anti-human6B11-PE, the relationship between iNKT cells and secretory otitis media in children was analyzed for the first time. Methods the peripheral blood, tonsillar and adenoid tissues of 10 normal children, 13 children with chronic tonsillitis and adenoid hypertrophy without SOM, 13 patients with secretory otitis media and 7 patients with tonsillar tissue were collected. Adenoid tissue in 12 cases. Specific antibody anti-human CD3-FITC and anti-human6B11-PE were used to label iNKT cells in peripheral blood and tissues. The percentage of iNKT cells in each specimen was detected by flow cytometry academic (FCM). Results 1 the percentage of iNKT cells in peripheral blood of normal children was (0.9660 卤0.4221)%, that of children without SOM was (1.5231 卤1.0226)%, that of children with chronic tonsillitis and adenoid hypertrophy was (1.5231 卤1.0226)%. The percentage of iNKT cells in peripheral blood of children with secretory otitis media was (1.5692 卤0.7200)%. By single factor analysis of variance, P < 0.05, the difference among the three groups was statistically significant. By LSD, there was significant difference between normal children and children with secretory otitis media, but there was no significant difference between the other two groups. 2Detection of iNKT cells in tonsils the percentage of iNKT cells in tonsils of children with secretory otitis media was (1.0105 卤0.5241)%. The percentage of iNKT cells in tonsil tissue of children with chronic tonsillitis without SOM was (0.8883 卤0.6419)%, which was calculated by t-test of two independent samples (P > 0.05), and there was no significant difference between the two groups. 3The percentage of adenoid tissue iNKT cells was (1.2658 卤0.8250)% in children with secretory otitis media and (0.8246 卤0.4289)% in adenoid tissues without SOM. After two independent samples t test, P > 0.05, there was no significant difference between the two groups. Conclusion it is feasible to label iNKT cells with anti-human CD3-FITC and anti-human6B11-PE. There was no significant difference in the proportion of iNKT cells in tonsils and adenoids between children with SOM and those with chronic tonsillitis without SOM and adenoid hypertrophy. The percentage of iNKT cells in peripheral blood of children with secretory otitis media was higher than that of normal children. It was suggested that iNKT cells might be related to the occurrence of secretory otitis media in children.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R764.21
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