病毒感染及相关免疫因子与鼻内翻性乳头状瘤发病关系的研究

发布时间：2018-12-28 09:38

【摘要】：目的:探讨人乳头状瘤病毒(HPV)感染及相关免疫因子TGF-β、IL-10、IFN-γ与鼻内翻性乳头状瘤(NIP)及鼻鳞状细胞癌(NSCC)的关系。方法:收集30例NIP(其中7例为复发性NIP)、23例NSCC、19例鼻息肉(NP)组织,采用原位杂交法检测HPV6/11、HPV16/18型的感染情况,采用免疫组织化学技术EnVision二步法检测TGF-β、IL-10、IFN-γ的表达情况。结果:30例NIP、23例NSCC的HPV感染阳性率分别为43.33%、52.17%,19例NP中无一例有HPV感染。23例非复发性NIP中有9例HPV感染,7例复发性NIP中有3例HPV感染。NIP与NP、NSCC与NP之间HPV感染率差异有统计学意义(P0.05),NIP与NSCC、非复发性NIP与复发性NIP之间HPV感染率差异无统计学意义(P0.05)。TGF-β、IL-10、IFN-γ因子在NIP、NSCC及NP组中均有表达。NIP、NSCC、NP组间TGF-β免疫组织化学染色程度存在差异(P0.01),NIP组比NP组、NSCC组间TGF-β的表达增加,NSCC组与NP组间TGF-β的表达无差异。NIP、NSCC、NP组间IL-10的表达存在差异(P0.01),NIP组比NP组IL-10的表达减少,NIP组与NSCC组间IL-10的表达程度无差异,NSCC组与NP组IL-10的表达程度无差异。NIP、NSCC、NP组间IFN-γ的表达程度无差异(P0.05)。NIP、NSCC组HPV感染与TGF-β、IL-10、IFN-γ的表达之间无差异(P0.05)。结论:HPV感染与部分NIP、NSCC的发生有关;HPV感染与NIP恶变为癌的关系尚不明确;HPV感染与NIP复发无关;TGF-β及IL-10的异常表达可能与NIP的发病有关;IFN-γ与NIP的发生、发展无关;HPV感染与NIP、NSCC中TGF-β、IL-10、IFN-γ的表达不相关。
[Abstract]:Objective: to investigate the relationship between (HPV) infection of human papillomavirus and TGF- 尾, IL-10,IFN- 纬, (NIP) and (NSCC) in nasal inverted papilloma. Methods: 30 cases of NIP (including 7 cases of recurrent NIP), and 23 cases of NSCC,19 nasal polyp (NP) were collected. The infection of HPV6/11,HPV16/18 type was detected by in situ hybridization. The expression of TGF- 尾 and IL-10,IFN- 纬 was detected by EnVision two step method. Results: the positive rates of HPV infection in NSCC of 30 cases of NIP,23 were 43.33% and 52.17% of 19 cases of NP, 9 cases of HPV infection in 23 cases of non-recurrent NIP and 3 cases of HPV infection in 7 cases of recurrent NIP. There was no significant difference in HPV infection rate between NSCC and NP (P0.05) between), NIP and NSCC, non-recurrent NIP and recurrent NIP (P0.05). TGF- 尾, IL-10,IFN- 纬 factor were found in NIP,. The expression of TGF- 尾 in NIP,NSCC,NP group was higher than that in NP group, but there was no difference in TGF- 尾 expression between NSCC group and NP group (P0.01), NIP group was higher than that in NP group, and there was no difference in NIP, 尾 expression between NSCC group and NP group. The expression of IL-10 in NSCC,NP group was significantly lower than that in NP group (P 0.01). There was no difference in IL-10 expression between NIP group and NSCC group, but there was no difference in NIP,NSCC, expression between NSCC group and NP group. There was no difference in the expression of IFN- 纬 between NP group and NIP,NSCC group (P0.05). There was no difference between HPV infection and TGF- 尾, IL-10,IFN- 纬 expression in NIP,NSCC group (P0.05). Conclusion: the relationship between HPV infection and partial NIP,NSCC, the relationship between HPV infection and the malignant transformation of NIP into cancer is not clear, there is no relationship between HPV infection and NIP recurrence, and the abnormal expression of TGF- 尾 and IL-10 may be related to the pathogenesis of NIP. IFN- 纬 was not related to the occurrence and development of NIP, but HPV infection was not related to the expression of TGF- 尾 and IL-10,IFN- 纬 in NIP,NSCC.
【作者单位】：福建医科大学附属第一医院耳鼻咽喉科;
【分类号】：R739.62

【相似文献】