喉返神经不同部位损伤后环杓后肌组织病理研究

发布时间：2018-12-29 11:55

【摘要】：目的:通过对环杓后肌组织病理形态的观察,探讨喉返神经不同部位损伤对喉返神经功能恢复的影响。方法:健康杂种犬8只平均分为4组,正常对照组只显露喉返神经不予损伤,实验组A组、B组、C组分别在入喉处、第4气管环水平处、近主动脉弓水平处钳夹左侧喉返神经。术后观察犬的发音、吞咽及呼吸功能情况,并于8周后取下环杓后肌,分别进行HE染色、Ach E染色及SDH染色,观察肌组织病理形态变化。结果:A组、B组、C组的红肌细胞数、白肌细胞数和红白肌细胞数比值较正常对照组呈递减趋势,但这3组数据在统计学上无显著差异性;A组、B组、C组的环杓后肌运动终板数较正常对照组呈递减趋势,且有明显统计学差异性。结论:喉返神经不同部位损伤可以导致神经功能恢复水平不同,损伤部位离靶器官越远,神经肌肉功能恢复越差。
[Abstract]:Objective: to investigate the effect of injury of recurrent laryngeal nerve on the recovery of recurrent laryngeal nerve by observing the histopathological morphology of posterior cricoarytenoid muscle. Methods: eight healthy mongrel dogs were divided into 4 groups on average. The normal control group only exposed the recurrent laryngeal nerve without injury. The experimental groups A, B and C were at the level of the larynx and the fourth tracheal ring, respectively. The left recurrent laryngeal nerve was clamped near the level of the aortic arch. The articulation, swallowing and respiratory function of dogs were observed after operation. The posterior cricoarytenoid muscle was removed 8 weeks later and stained with HE, Ach E and SDH respectively. The histopathological changes of muscle were observed. Results: the number of red muscle cells, white muscle cells and the ratio of red and white muscle cells in group A, group B and group C were decreased compared with those in control group, but there was no statistical difference among the three groups. In group A, group B and group C, the number of posterior cricoarytenoid motor endplates was decreased compared with that of normal control group, and there was significant difference in the number of motor endplates between group A, group B and group C. Conclusion: different parts of recurrent laryngeal nerve injury can lead to different recovery level of nerve function, the farther away from target organ, the worse the recovery of neuromuscular function.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R767.8

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