依那西普滴眼液用于小鼠干眼症的效果及其机制

发布时间：2019-01-17 12:57

【摘要】：目的观察依那西普滴眼液用于小鼠干眼症的效果,并探讨其作用机制。方法将苯扎氯铵溶液诱导的30只干眼症小鼠随机分为干眼组、PBS组、依那西普组,每组10只。干眼组不予任何处理,PBS组、依那西普组分别给予PBS、0.1%依那西普滴眼液滴眼,连用14天。滴眼前(第0天)及滴眼第7、14天检测泪液分泌量、泪膜破裂时间和角膜荧光素钠染色评分(角膜染色评分)。滴眼第14天处死小鼠,检测结膜组织IL-1β、IL-17、TNF-α表达。结果各组滴眼前泪液分泌量、泪膜破裂时间和角膜染色评分比较差异无统计学意义(P均0.05)。与滴眼前比较,依那西普组滴眼第7、14天泪液分泌量增加、泪膜破裂时间明显延长,角膜染色评分明显降低(P均0.05),干眼组各指标变化不明显(P均0.05);与PBS组、干眼组滴眼第7、14天比较,依那西普组同时间点泪液分泌量增加、泪膜破裂时间明显延长,角膜染色评分明显降低(P均0.05),而PBS组、干眼组同期上述各指标比较差异均无统计学意义(P均0.05)。滴眼第14天,依那西普组结膜组织上清液炎症因子IL-1β、IL-17和TNF-α水平明显低于干眼组(P均0.05),而PBS组、干眼组比较差异均无统计学意义(P均0.05)。结论依那西普滴眼液可减轻苯扎氯铵诱导的干眼症小鼠的临床症状,其机制可能与抑制IL-1β、IL-17、TNF-α等炎症因子水平有关。
[Abstract]:Objective to observe the effect of Einasepine eye drops on dry eye in mice and to explore its mechanism. Methods Thirty mice with dry eye induced by benzalkonium chloride solution were randomly divided into three groups: dry eye group, PBS group and etanisepine group, with 10 mice in each group. Dry eye group was not treated, PBS group and Einacetin group were given PBS,0.1% etanisepine eye drops for 14 days. Tear secretion, tear film rupture time and corneal fluorescein staining score (corneal staining score) were measured at day 7 and day 14 respectively. On the 14th day, the mice were killed and the expression of IL-1 尾 and IL-17,TNF- 伪 in conjunctival tissue was detected. Results there was no significant difference in tear discharge, tear film rupture time and corneal staining score in all groups (P 0.05). Compared with the eyes, the tear secretion increased, the tear film rupture time was prolonged, the corneal staining score was significantly decreased (P 0.05), and the indexes of dry eye group were not changed significantly (all P 0.05). Compared with PBS group, dry eye group, tear secretion increased, tear film rupture time prolonged and corneal staining score decreased significantly at the same time point in Einasepine group (P 0.05), while in PBS group, corneal staining score decreased significantly (P < 0. 05), but that in Einaseptide group was significantly lower than that in dry eye group at the same time (P < 0. 05). There was no significant difference between the above indexes in the dry eye group at the same time (P 0.05). On the 14th day, the levels of inflammatory factors IL-1 尾, IL-17 and TNF- 伪 in the conjunctival supernatant in the etanapril group were significantly lower than those in the dry eye group (P 0.05), but there was no significant difference between the PBS group and the dry eye group (P 0.05). Conclusion Einasepine eye drops can relieve the clinical symptoms of mice with xerophthalmia induced by benzalkonium chloride, and its mechanism may be related to the inhibition of IL-1 尾, IL-17,TNF- 伪 and other inflammatory factors.
【作者单位】：锦州医科大学附属第一医院;上海健康医学院附属第六人民医院东院;
【基金】：上海健康医学院种子基金项目(HMSF-16-21-013)
【分类号】：R777.34

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