短波视蛋白在豚鼠频闪光诱导性近视和形觉剥夺近视眼模型中的表达差异

发布时间：2019-01-27 07:31

【摘要】：目的观察豚鼠频闪光诱导性近视和形觉剥夺近视模型中短波视蛋白(S-opsin)表达差异,并初步探讨原因。方法 36只普通级2周龄豚鼠随机分成三组:频闪组(FLM组,n=13),形觉剥夺组(FDM组,n=12),对照组(n=11)。FLM组,饲养笼具安装有频闪仪(频率0.5 Hz),笼具内装有发光二极管;FDM组豚鼠右眼用半透明眼罩遮盖,并确保豚鼠眼睑能正常活动;对照组豚鼠不予特殊处理。在造模第1天(0周)和第6周测量豚鼠右眼屈光度、眼轴长度和角膜曲率半径,并通过免疫荧光法观察S-opsin表达。结果第0周,FLM、FDM组与对照组屈光度、眼轴长度、角膜曲率半径差异均无显著性(P0.05)。造模6周后,与对照组相比,FLM组、FDM组屈光度变化值、眼轴长度变化值差异均有显著性(P0.05),而角膜曲率半径变化值差异无显著性(P=0.358),提示成功建立近视模型。FLM组与FDM组相比,屈光度变化值、眼轴长度变化值、角膜曲率半径变化值差异均无显著性(P0.05)。免疫荧光结果显示:FLM组视蛋白灰度值对照组视蛋白灰度值FDM组视蛋白灰度值,任意两组进行比较,差异均有显著性(P0.001)。结论频闪光和形觉剥夺均能建立近视模型,频闪光诱导性近视模型中S-opsin产生增加,而形觉剥夺性近视模型中S-opsin产生减少,说明两种近视模型的发生机制可能不同。
[Abstract]:Objective to observe the difference of short wave protein (S-opsin) expression in guinea pigs with frequent flash induced myopia and form deprivation myopia, and to explore the causes. Methods Thirty-six normal 2-week-old guinea pigs were randomly divided into three groups: stroboscopic group (FLM group, n = 13), form deprivation group (FDM group, n = 12) and control group (n = 11). FLM), with stroboscopic device (frequency 0.5 Hz),). A light-emitting diode is arranged in the cage; The right eye of FDM group was covered with translucent goggles to ensure the normal movement of the eyelids of guinea pigs, while that of the control group was not treated with special treatment. The diopter, axial length and corneal curvature radius of the right eye of guinea pigs were measured on day 1 (0 week) and week 6 of the model, and the expression of S-opsin was observed by immunofluorescence method. Results at week 0, there was no significant difference in diopter, axial length and corneal curvature radius between FLM,FDM group and control group (P0.05). After 6 weeks of modeling, there were significant differences in diopter and axial length between FLM group and FDM group compared with control group (P0.05), but there was no significant difference in corneal curvature radius between FDM group and FLM group (P0. 358). The results showed that there was no significant difference in diopter, axial length and corneal curvature radius between FLM group and FDM group (P0.05). The results of immunofluorescence showed that there were significant differences between any two groups (P0. 001). The visual protein grayscale value of FLM group, control group, FDM group was significantly higher than that of any other group (P0. 001). Conclusion both frequent flash and form deprivation can establish myopia models. The S-opsin production increases in the frequent flash induced myopia model, while the S-opsin production decreases in the form deprivation myopia model, which indicates that the two myopia models may have different mechanisms.
【作者单位】：复旦大学附属金山医院;
【基金】：上海市自然科学基金(编号:17ZR1404200) 上海市卫计委项目(编号:201640046)
【分类号】：R778.11;R-332

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