大鼠局灶性脑缺血再灌注后听觉中枢损伤的研究

发布时间：2019-01-27 23:19

【摘要】：目的探讨大鼠局灶性脑缺血再灌注损伤后听觉中枢的改变及引起听力损伤的可能机制。方法选择健康白色雄性SD大鼠60只,随机分为假手术组和缺血再灌注组,每组30只。采用线栓法制备大鼠大脑中动脉栓塞模型,假手术组只分离颈部血管,不插入线栓。脑缺血60min,再灌注24h。于手术前及术后24h检测听性脑干反应,术后24h行神经功能评分,干湿重法测定脑组织含水量,TTC法检测脑梗死体积,通过Evans blue的渗出情况评估血脑屏障的完整性,末端标记法观察神经细胞凋亡状况,计算凋亡指数。Western blotting法测定MMP-9、Clau-din-5、Occludin、PSD-95、CX-43及Na+-α蛋白表达。结果与假手术组比较,缺血再灌注组神经功能评分及脑含水量升高,脑梗死体积增大,ABR反应阈值明显增加,凋亡指数增加,MMP-9、CX-43蛋白表达明显上升,Claudin-5、Occludin、PSD-95、Na+-α蛋白表达显著下降。两组比较差异均具有统计学意义。结论局灶性脑缺血再灌注损伤后听觉中枢的改变及听力损伤可能与MMPs激活、血脑屏障破坏、缝隙连接通道功能活化、突触功能异常及离子通道破坏有关。
[Abstract]:Objective to investigate the changes of auditory center and the possible mechanism of hearing impairment after focal cerebral ischemia reperfusion injury in rats. Methods 60 healthy white male SD rats were randomly divided into sham operation group and ischemia reperfusion group with 30 rats in each group. The middle cerebral artery embolization model of rats was established by thread embolization. The sham-operated group only separated cervical blood vessels and did not insert thread embolism. Cerebral ischemia for 60 min and reperfusion for 24 h. Auditory brainstem reaction was detected before and 24 hours after operation. Nerve function score, water content of brain tissue were measured by dry and wet weight method, cerebral infarction volume was measured by TTC method, and the integrity of blood-brain barrier was evaluated by the exudation of Evans blue. The apoptosis of nerve cells was observed by end labeling method, and the expression of MMP-9,Clau-din-5,Occludin,PSD-95,CX-43 and Na-伪 protein was measured by. Western blotting method. Results compared with the sham-operation group, the neurological function score and cerebral water content in the ischemia-reperfusion group increased, the volume of cerebral infarction increased, the threshold of ABR reaction increased, the apoptotic index increased, the expression of MMP-9,CX-43 protein increased, and the expression of Claudin-5,Occludin, increased. The expression of PSD-95,Na-伪 protein decreased significantly. The difference between the two groups was statistically significant. Conclusion the changes of auditory center and hearing loss after focal cerebral ischemia-reperfusion injury may be related to the activation of MMPs, the destruction of blood-brain barrier, the activation of gap junction channel, the abnormal synaptic function and the destruction of ion channel.
【作者单位】：河北医科大学第二医院耳鼻咽喉科;
【分类号】：R764

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