铁碳纳米粒介导顺铂对喉癌细胞株化疗敏感性影响的研究

发布时间：2019-02-13 13:44

【摘要】：目的探讨铁碳纳米微粒荷载顺铂对喉癌Hep-2细胞的增殖抑制作用的影响及其作用机制。方法选择喉癌Hep-2细胞进行复苏及传代，对数生长期Hep-2细胞分为对照组、微粒组、药物组及微粒药物组，各组分别加入处理液，对照组是生理盐水，药物组的处理液为顺铂生理盐水溶液，微粒药物组为铁碳纳米粒顺铂生理盐水分散液，微粒组是铁碳纳米微粒生理盐水分散液。细胞培养24及48h后计算各组细胞的生长抑制率，细胞培养3天后观察细胞形态，细胞培养5天后采用RT-PCR技术检测各组细胞Caspase3mRNA及Survivin mRNA的表达情况，比较各组细胞生长抑制率，细胞生长形态及各组细胞Caspase3mRNA及Survivin mRNA的表达水平的差异。结果各组细胞加入处理液培养24小时后，对照组、微粒组、药物组及微粒药物组细胞生长抑制率分别为4.1%、4.7%、19.6%和25.6%，微粒药物组及药物组细胞生长抑制率高于对照组及微粒组，微粒药物组生长抑制率高于药物组，对照组同微粒组生长抑制率无显著差异。各组细胞培养48小时后，对照组、微粒组、药物组及微粒药物组细胞生长抑制率分别为4.0%、4.5%、37.2%和51.4%，微粒药物组及药物组细胞生长抑制率高于对照组及微粒组，微粒药物组生长抑制率高于药物组，对照组同微粒组生长抑制率无显著差异。细胞培养3天后，微粒药物组及药物组细胞生长抑制，表现为细胞生长不贴壁，增殖缓慢，出现凋亡征象，微粒药物组细胞生长抑制较药物组更为明显，各组喉癌Hep-2细胞培养5天后RT-PCR检测Caspase3mRNA和Survivin mRNA表达水平结果显示，对照组、微粒组、药物组及微粒药物组细胞Caspase3mRNA表达水平分别为0.84±0.14、0.87±0.16、1.41±0.25和1.93±0.27，药物组及微粒药物组细胞Caspase3mRNA表达水平高于微粒组及对照组，，微粒药物组细胞Caspase3mRNA表达水平高于药物组，对照组、微粒组、药物组及微粒药物组细胞SurvivinmRNA表达水平水平分别为1.34±0.17、1.29±0.13、0.84±0.19和0.52±0.14，药物组及微粒药物组细胞Survivin mRNA表达水平低于对照组及微粒组，微粒药物组细胞Survivin mRNA表达水平低于药物组，微粒组同对照组Caspase3mRNA、Survivin mRNA表达水平无显著差异。结论铁碳纳米微粒搭载顺铂能够增加喉癌Hep-2细胞Caspase3mRNA的表达，降低Survivin mRNA的表达，增加细胞生长抑制率，增加喉癌Hep-2细胞对顺铂的敏感性，提高药物化疗效果。
[Abstract]:Objective to investigate the effect of iron carbon nanoparticles loading cisplatin on the proliferation inhibition of laryngeal carcinoma Hep-2 cells and its mechanism. Methods Hep-2 cells of laryngeal carcinoma were selected for resuscitation and passage. Hep-2 cells in logarithmic growth phase were divided into control group, microparticle group, drug group and microparticle drug group. The treatment solution of drug group was cisplatin normal saline solution, the particle drug group was iron carbon nanoparticles cisplatin normal saline dispersion, and the particle group was iron carbon nanoparticles normal saline dispersion. After 24 and 48 hours of cell culture, the growth inhibition rate of each group was calculated, the cell morphology was observed after 3 days of cell culture, the expression of Caspase3mRNA and Survivin mRNA was detected by RT-PCR technique after 5 days of cell culture, and the growth inhibition rate of each group was compared. The difference of cell growth morphology and the expression of Caspase3mRNA and Survivin mRNA in each group. Results after the cells were cultured in the treatment solution for 24 hours, the cell growth inhibition rates of the control group, the microparticle group, the drug group and the microparticle drug group were 4.1% and 25.6%, respectively. The cell growth inhibition rate of the microparticle drug group and the drug group was higher than that of the control group and the microparticle group, and the growth inhibition rate of the particle drug group was higher than that of the drug group, but there was no significant difference between the control group and the particle group. After 48 hours of culture, the cell growth inhibition rates of control group, microparticle group, drug group and microparticle drug group were 4.0% and 51.4%, respectively. The cell growth inhibition rate of the microparticle drug group and the drug group was higher than that of the control group and the microparticle group, and the growth inhibition rate of the particle drug group was higher than that of the drug group, but there was no significant difference between the control group and the particle group. After 3 days of cell culture, cell growth inhibition was observed in the microparticle drug group and the drug group, showing that cell growth was not adhered to the wall, proliferation was slow, and apoptosis was observed. The cell growth inhibition in the microparticle drug group was more obvious than that in the drug group. The expression levels of Caspase3mRNA and Survivin mRNA in Hep-2 cells of laryngeal carcinoma were detected by RT-PCR 5 days later. The results showed that the expression of Caspase3mRNA and Survivin mRNA in the control group and the microparticle group were measured. The expression levels of Caspase3mRNA were 0.84 卤0.140.87 卤0.16 卤0.41 卤0.25 and 1.93 卤0.27 in drug group and microparticle drug group, respectively. The expression level of Caspase3mRNA in drug group and microparticle drug group was higher than that in microparticle group and control group. The expression level of Caspase3mRNA in the microparticle drug group was higher than that in the drug group. The SurvivinmRNA expression levels in the control group, the microparticle group, the drug group and the microparticle drug group were 1.34 卤0.17, 1.29 卤0.13, 0.84 卤0.19 and 0.52 卤0.14, respectively. The expression level of Survivin mRNA in the drug group and microparticle group was lower than that in the control group and microparticle group. The expression level of Survivin mRNA in the microparticle drug group was lower than that in the drug group. There was no significant difference between the microparticle group and the control group in the expression of Caspase3mRNA,Survivin mRNA. Conclusion Cisplatin in iron carbon nanoparticles can increase the expression of Caspase3mRNA, decrease the expression of Survivin mRNA, increase the growth inhibition rate, increase the sensitivity of Hep-2 cells to cisplatin, and improve the effect of drug chemotherapy.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.65

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