大鼠玻璃体腔内注射尿激酶对视网膜血管通透性的影响

发布时间：2019-03-17 07:28

【摘要】：目的研究大鼠玻璃体腔内注射尿激酶(UK)对视网膜血管通透性的影响。方法 SD健康大鼠30只,随机分为2组,每组15只(15眼)。实验组大鼠右眼玻璃体腔内注射UK350U/4u l,对照组大鼠右眼玻璃体腔内注射0.01M磷酸盐缓冲液(PBS)4μ l。24小时后大鼠尾静脉注射追踪剂依文思蓝(EB),待EB循环2小时后,摘除大鼠右眼球,取出玻璃体,使用间接酶联免疫吸附试验(ELISA)检测玻璃体内EB吸光度。根据EB浓度-吸光度的标准曲线,获得大鼠玻璃体腔内EB的质量浓度,采用SPSS17.0对数据进行统计学分析。结果 1.统计学软件制作依文思蓝的浓度-吸光度标准曲线可以看出：依文思蓝的浓度在0-30μ g/ml,吸光度在0.008-0.12的范围内线性关系良好,回归方程为y=0.0034x+0.0044,R2=0.9941,n=6。 2.显微镜下观察大鼠右眼球,可见实验组大鼠眼球颜色明显蓝于对照组大鼠眼球。实验组大鼠眼球普遍为蓝色,个体间颜色差异较小；对照组大鼠眼球基本上无色,少数眼球稍蓝。 3. ELISA法测得大鼠玻璃体内EB的吸光度结果显示：实验组大鼠玻璃体腔内EB平均吸光度值为(0.0558±0.0112)/25μ l,对照组大鼠玻璃体腔内EB的平均吸光度值为(0.0145±0.0077)/25μ l,两组差异有统计学意义(t=11.746,P=0.000)。 4.由EB浓度-吸光度标准曲线得出大鼠玻璃体内EB的平均质量浓度结果显示：实验组大鼠玻璃体内EB的平均质量浓度为(15.1078±3.2905)u g/25μ l,对照组大鼠玻璃体腔内EB的平均质量浓度为(3.3093±2.1615)μ g/25u l,二组差异有统计学意义(t=11.607,P=0.000)。结论 1、大鼠玻璃体腔内注射UK可以增大视网膜血管的通透性 2、EB可以作为一种测量视网膜血管通透性的理想追踪剂
[Abstract]:Objective to study the effect of intravitreal injection of urokinase (UK) on retinal vascular permeability in rats. Methods 30 healthy SD rats were randomly divided into two groups with 15 eyes in each group. Rats in the experimental group received intravitreal injection of UK350U/4u-1 in the right eye, while in the control group, 0.01m phosphate buffer solution (PBS) was injected into the vitreous cavity of the control group for 24 hours after injection of the tracking agent Evans blue (EB), into the tail vein of the rats. After 2 hours of EB circulation, the right eyeball of the rat was removed, the vitreous body was removed, and the EB absorbance in the vitreous was detected by indirect enzyme-linked immunosorbent assay (ELISA). According to the standard curve of EB concentration-absorbance, the mass concentration of EB in rat vitreous cavity was obtained, and the data were analyzed statistically by SPSS17.0. Outcome 1. The standard curve of concentration-absorbance of Evans blue produced by statistical software shows that the linear relationship between the concentration of Evans blue is 0-30 渭 g / ml, the absorbance is 0. 008 渭 g / ml, and the regression equation is y = 0. 0034 4 x 0. 0044, and the regression equation is y = 0. 0034 x 0. 0044. R _ (2), n ~ (2) 0. 941, n (?) 6. 2. The right eyeball of the rats was observed under microscope. The eye color of the experimental group was obviously blue than that of the control group. The eyes of the experimental group were generally blue with little color difference among individuals, while those of the control group were basically colorless and a few of the eyeballs were a little blue. 3. The absorbance of rat vitreous EB measured by ELISA showed that the average absorbance value of EB in vitreous cavity of experimental group was (0.0558 卤0.0112) / 25 渭 l, and the absorbance of EB in vitreous cavity was (0.0558 卤0.0112) / 25 渭 l in experimental group. The average absorbance of EB in vitreous cavity of the control group was (0.0145 卤0.0077) / 25 渭 l, and there was a significant difference between the two groups (t = 11.746, P = 0.000). 4. According to the EB concentration-absorbance standard curve, the average mass concentration of EB in the vitreous of rats in the experimental group was (15.1078 卤3.2905) ug / 25 渭 l, the average concentration of EB in the vitreous of the experimental group was (15.1078 卤3.2905) ug / 25 渭 l. The mean mass concentration of EB in vitreous cavity of the control group was (3.3093 卤2.1615) 渭 g / L, and there was a significant difference between the two groups (t = 11.607, P = 0.000). Conclusion 1. Intravitreal injection of UK can increase retinal vascular permeability 2. EB can be used as an ideal tracer to measure retinal vascular permeability.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R774.1

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