维生素A对光损伤小鼠视网膜功能和结构的保护作用

发布时间：2019-03-17 09:00

【摘要】：目的探索维生素A对光损伤后小鼠视网膜结构和功能的保护作用。方法将12只清洁级雄性成年C57小鼠随机分为生理盐水组和维生素A组。2组小鼠于实验前行双眼视网膜电图(ERG)标准五项反应检测视网膜功能,2组小鼠分别接受1m L·kg-1生理盐水或维生素A(0.1%)腹腔内注射,连续5 d。2组小鼠按照动物光损伤模型标准制作右眼单眼光损伤模型,左眼遮盖,造模成功后继续饲养5 d,重复检测ERG。功能检测后立即取小鼠双侧眼球做视网膜组织切片,比较2组小鼠视网膜结构的变化。结果实验前2组小鼠双眼暗适应0.01ERG b波、暗适应3.0ERG a和b波、暗适应3.0震荡电位的总幅值、明适应3.0ERG b波及3.0闪烁光反应P1波总幅值的差异均无统计学意义(P均0.05)。光损伤造模后,两组小鼠右眼与自身对照左眼相比,上述ERG 5项检测的波幅值均出现明显下降(P均0.05);维生素A组小鼠右眼暗适应0.01ERG的b波、暗适应3.0ERG的a波和b波、暗适应3.0震荡电位的总幅值均高于生理盐水组(t=3.544,P=0.019;t=4.574,P=0.011;t=9.563,P=0.002;t=6.156,P=0.002),而明适应3.0 ERG的b波以及闪烁光反应P1波总幅值的差异无统计学意义(t=4.678,P=0.469;t=4.883,P=0.342);2组小鼠右眼的视网膜外核层(ONL)细胞层数较自身对照左眼均有所减少(P0.05),在生理盐水组小鼠右眼的视网膜外核层(ONL)细胞层数(6.5±1.05)少于维生素A组(11.5±1.03)(t=2.301,P=0.003)。结论维生素A对光照引起的小鼠视网膜结构和功能损伤有一定的保护作用。
[Abstract]:Objective to explore the protective effect of vitamin A on retinal structure and function after light injury in mice. Methods Twelve adult male C57 mice of clean grade were randomly divided into saline group and vitamin A group. Before the experiment, two groups of mice were examined with binocular electroretinogram (ERG) standard five reactions. The mice in the two groups were given intraperitoneal injection of 1 L kg-1 saline or vitamin A (0.1%) respectively for 5 days. The right eye injury model was made according to the standard of the animal light injury model, and the left eye was covered by the left eye, and the right eye injury model was established according to the standard of the animal light injury model. Continue feeding for 5 days after successful modeling, repeat detection of ERG. After functional examination, the bilateral eyeballs of the mice were taken for retinal tissue section, and the changes of retinal structure in the two groups were compared. Results before the experiment, the 0.01ERG b wave, 3.0ERG a and b waves, and the total amplitude of the shock potential of the two groups were adapted to dark adaptation, and dark adaptation to 3.0ERG a and b waves, respectively. There was no significant difference in the total amplitudes of P1 wave in light adaptation to 3.0ERG b and 3.0 scintillation light response (P0.05). After light injury, the amplitudes of the above-mentioned ERG 5 items decreased significantly in the right eye of the two groups compared with the left eye of the self-control group (P0.05). In vitamin A group, the right eye adapted to the b wave of 0.01ERG in dark, a wave and b wave of 3.0ERG in dark, and the total amplitude of shock potential in dark adaptation was higher than that in physiological saline group (t = 3.54 4, P = 0.019 / t / 4.574, P = 0.011). T = 9.563, P = 0.002t / 6.156, P = 0.002), but there was no significant difference in the total amplitudes of P _ 1 wave (t = 4.678, P = 0.469 / t / 4.883, P = 0.342) at 3.0 ERG (t = 4.678, P = 0.469 / t / 4.883, P = 0.342). The number of (ONL) cell layers in the outer retinal layer of the right eye in the two groups was lower than that in the left eye of the self-control group (P0.05). The number of (ONL) cell layers in the extraretinal layer of the right eye in the saline group was lower than that in the vitamin A group (6.5 卤1.05) (t = 2.301, P = 0.003). Conclusion Vitamin A can protect the retinal structure and function of mice induced by light.
【作者单位】：宁夏医科大学总医院眼科;第四军医大学航空医学系视觉电生理学教研室;
【分类号】：R774.1

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